TY - JOUR
T1 - Antimicrobial Activity of Methylene Blue Associated with Photodynamic Therapy
T2 - In Vitro Study in Multi-Species Oral Biofilm
AU - Bueno-Silva, Bruno
AU - Parma-Garcia, Javier
AU - Frigo, Lucio
AU - Suárez, Lina J.
AU - Macedo, Tatiane Tiemi
AU - Uyeda, Fábio Hideaki
AU - Melo, Marcelo Augusto Ruiz da Cunha
AU - Sacco, Roberto
AU - Mourão, Carlos Fernando
AU - Feres, Magda
AU - Shibli, Jamil Awad
AU - Figueiredo, Luciene Cristina
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/4
Y1 - 2024/4
N2 - The control of infectious diseases caused by biofilms is a continuing challenge for researchers due to the complexity of their microbial structures and therapeutic implications. Photodynamic therapy as an adjunctive anti-infective treatment has been described as a possible valid approach but has not been tested in polymicrobial biofilm models. This study evaluated the effect of photodynamic therapy in vitro with methylene blue (MB) 0.01% and red LEDs (λ = 660 nm, power density ≈ 330 mW/cm2, 2 mm distance from culture) on the metabolic activity and composition of a multispecies subgingival biofilm. Test Groups LED and MB + LED showed a more significant reduction in metabolic activity than the non-LED application group (~50 and 55%, respectively). Groups LED and MB equally affected (more than 80%) the total bacterial count in biofilms. No differences were noted in the bacterial biofilm composition between the groups. In vitro LED alone or the MB + LED combination reduced the metabolic activity of bacteria in polymicrobial biofilms and the total subgingival biofilm count.
AB - The control of infectious diseases caused by biofilms is a continuing challenge for researchers due to the complexity of their microbial structures and therapeutic implications. Photodynamic therapy as an adjunctive anti-infective treatment has been described as a possible valid approach but has not been tested in polymicrobial biofilm models. This study evaluated the effect of photodynamic therapy in vitro with methylene blue (MB) 0.01% and red LEDs (λ = 660 nm, power density ≈ 330 mW/cm2, 2 mm distance from culture) on the metabolic activity and composition of a multispecies subgingival biofilm. Test Groups LED and MB + LED showed a more significant reduction in metabolic activity than the non-LED application group (~50 and 55%, respectively). Groups LED and MB equally affected (more than 80%) the total bacterial count in biofilms. No differences were noted in the bacterial biofilm composition between the groups. In vitro LED alone or the MB + LED combination reduced the metabolic activity of bacteria in polymicrobial biofilms and the total subgingival biofilm count.
KW - biofilms
KW - laser
KW - methylene blue
KW - peri-implantitis
KW - periodontal diseases
KW - photodynamic therapy
UR - http://www.scopus.com/inward/record.url?scp=85191612682&partnerID=8YFLogxK
U2 - 10.3390/pathogens13040342
DO - 10.3390/pathogens13040342
M3 - Article
AN - SCOPUS:85191612682
SN - 2076-0817
VL - 13
JO - Pathogens
JF - Pathogens
IS - 4
M1 - 342
ER -