Resumen
Objectives: To compare the expression of tissue factor and thrombomodulin mRNA content in exosomes isolated from placental explant from normal and complicated pregnancies with preeclampsia.
Methods: We used twenty-one placental explant cultures of pregnant women without complications with healthy newborns (n=7), pregnant women diagnosed with early onset preeclampsia (n=7) and late onset preeclampsia (n=7). The exosome isolation was performed by ultracentrifugation. Quantification of exosomes was performed by ELISA (ExoQuant™ Overall Exosome Capture and Quantification Assay Kit). The exosome RNA extraction was done with the Total Exosome RNA and Protein Isolation Kit. Measurement of mRNA expression of tissue factor (TF) and thrombomodulin (TM) was performed by real-time PCR
Results: We found that the pregnant women with late onset PE have more concentration of exosomes isolated from placental explants compare to pregnant women with early onset PE and controls (P=0.0115). We found TF mRNA within exosomes in 57% of samples in control group, 43% in early PE and 86% in late PE groups. We found TM mRNA within exosomes in 43% of samples from control and early onset PE groups, and 57% of samples from late onset PE group, without statistically significant differences. There were no statistically significant differences in the amount of TM mRNA of exosomes between the groups. There were statistically significant differences in mRNA quantity of TF contained in isolated exosomes between early and late onset PE groups (P=0.0476), however there were no differences when compared to control group.
Conclusion: This is the first time that the presence of TF and TM mRNA contained in isolated exosomes from human placental explant cultures has been described. The increase of TF mRNA in exosomes from pregnant women with late onset PE may be involved in pathophysiology of the preeclampsia in this presentation of the disease.
Methods: We used twenty-one placental explant cultures of pregnant women without complications with healthy newborns (n=7), pregnant women diagnosed with early onset preeclampsia (n=7) and late onset preeclampsia (n=7). The exosome isolation was performed by ultracentrifugation. Quantification of exosomes was performed by ELISA (ExoQuant™ Overall Exosome Capture and Quantification Assay Kit). The exosome RNA extraction was done with the Total Exosome RNA and Protein Isolation Kit. Measurement of mRNA expression of tissue factor (TF) and thrombomodulin (TM) was performed by real-time PCR
Results: We found that the pregnant women with late onset PE have more concentration of exosomes isolated from placental explants compare to pregnant women with early onset PE and controls (P=0.0115). We found TF mRNA within exosomes in 57% of samples in control group, 43% in early PE and 86% in late PE groups. We found TM mRNA within exosomes in 43% of samples from control and early onset PE groups, and 57% of samples from late onset PE group, without statistically significant differences. There were no statistically significant differences in the amount of TM mRNA of exosomes between the groups. There were statistically significant differences in mRNA quantity of TF contained in isolated exosomes between early and late onset PE groups (P=0.0476), however there were no differences when compared to control group.
Conclusion: This is the first time that the presence of TF and TM mRNA contained in isolated exosomes from human placental explant cultures has been described. The increase of TF mRNA in exosomes from pregnant women with late onset PE may be involved in pathophysiology of the preeclampsia in this presentation of the disease.
| Idioma original | Inglés |
|---|---|
| Páginas (desde-hasta) | E104-E104 |
| Número de páginas | 1 |
| Publicación | Placenta |
| Volumen | 83 |
| DOI | |
| Estado | Publicada - ago. 2019 |
ODS de las Naciones Unidas
Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible
