The IL-4 rapidly produced in BALB/c mice after infection with leishmania major down-regulates IL-12 receptor β2-chain expression on CD4⁺ T cells resulting in a state of unresponsiveness to IL-12

Within 1 day of infection with Leishmania major, susceptible BALB/c mice produce a burst of IL-4 in their draining lymph nodes, resulting in a state of unresponsiveness to IL-12 in parasite-specific CD4⁺ T cells within 48 h. In this report we examined the molecular mechanism underlying this IL-12 unresponsiveness. Extinction of IL-12 signaling in BALB/c mice is due to a rapid down-regulation of IL-12R β2-chain mRNA expression in CD4⁺ T cells. In contrast, IL-12R β2-chain mRNA expression was maintained on CD4⁺ T cells from resistant C57BL/6 mice. The down-regulation of the IL-12R β2-chain mRNA expression in BALB/c CD4⁺ T cells is a consequence of the early IL-4 production. In this murine model of infection, a strict correlation is shown in vivo between expression of the IL-12R β2-chain in CD4⁺ T cells and the development of a Th1 response and down-regulation of the mRNA β2-chain expression and the maturation of a Th2 response. Treatment of BALB/c mice with IFN-γ even when IL-4 has been produced for 48 h, resulted in maintenance of IL-12R β2-chain mRNA expression and IL-12 responsiveness. The data presented here support the hypothesis that the genetically determined susceptibility of BALB/c mice to infection with L. major is primarily based on an up-regulation of IL-4 production, which secondarily induces extinction of IL-12 signaling.