TY - JOUR
T1 - Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells
AU - Ballesteros-Vivas, D.
AU - Alvarez-Rivera, Gerardo
AU - García Ocampo, Andrés Felipe
AU - Morantes, Sandra Johanna
AU - Sánchez Camargo, Andrea del Pilar
AU - Cifuentes, Alejandro
AU - Parada-Alfonso, Fabián
AU - Ibánez, E.
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10
Y1 - 2019/10
N2 - Mango seed kernel (MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and 0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.
AB - Mango seed kernel (MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and 0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.
KW - Antiproliferative activity
KW - Fruit by-products
KW - HT-29 colon cancer cell line
KW - High-resolution mass spectrometry
KW - LC-q-TOF-MS
KW - Mangifera indica L
KW - Pressurized-liquid extraction
KW - Supercritical antisolvent fractionation
UR - http://www.scopus.com/inward/record.url?scp=85068250476&partnerID=8YFLogxK
U2 - 10.1016/j.supflu.2019.104563
DO - 10.1016/j.supflu.2019.104563
M3 - Article
AN - SCOPUS:85068250476
SN - 0896-8446
VL - 152
JO - Journal of Supercritical Fluids
JF - Journal of Supercritical Fluids
M1 - 104563
ER -
