Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides

Hernando Curtidor; Marisol Ocampo; Diana Tovar; Ramses López; Javier García; Jhon Valbuena; Ricardo Vera; Jorge Suárez; Luis E. Rodríguez; Álvaro Puentes; Fanny Guzmán; Elizabeth Torres; Manuel E. Patarroyo

doi:10.1016/j.vaccine.2003.07.019

Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides

Hernando Curtidor, Marisol Ocampo, Diana Tovar, Ramses López, Javier García, Jhon Valbuena, Ricardo Vera, Jorge Suárez, Luis E. Rodríguez, Álvaro Puentes, Fanny Guzmán, Elizabeth Torres, Manuel E. Patarroyo

Universidad Nacional de Colombia

Producción: Contribución a una revista › Artículo › revisión exhaustiva

13 Citas (Scopus)

Resumen

Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activity binding peptides (HABPs) were identified; 26201, 26202, 26203 and 26204 spanned residues ⁴⁶¹C-K⁵⁴⁰ within RAP1 Cys region, whilst 26188 (²⁰¹T-Y²²⁰) was located in p67 amino terminal. The results showed that peptide binding was saturable, some HABPs inhibited in vitro merozoite invasion and specifically bound to a 72kDa protein in red blood cell membrane. HABP possible function in merozoite invasion of erythrocytes is also discussed.

Idioma original	Inglés
Páginas (desde-hasta)	1054-1062
Número de páginas	9
Publicación	Vaccine
Volumen	22
N.º	8
DOI	https://doi.org/10.1016/j.vaccine.2003.07.019
Estado	Publicada - 25 feb. 2004
Publicado de forma externa	Sí

ODS de las Naciones Unidas

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Curtidor, H., Ocampo, M., Tovar, D., López, R., García, J., Valbuena, J., Vera, R., Suárez, J., Rodríguez, L. E., Puentes, Á., Guzmán, F., Torres, E., & Patarroyo, M. E. (2004). Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides. Vaccine, 22(8), 1054-1062. https://doi.org/10.1016/j.vaccine.2003.07.019

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title = "Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides",

abstract = "Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activity binding peptides (HABPs) were identified; 26201, 26202, 26203 and 26204 spanned residues 461C-K540 within RAP1 Cys region, whilst 26188 (201T-Y220) was located in p67 amino terminal. The results showed that peptide binding was saturable, some HABPs inhibited in vitro merozoite invasion and specifically bound to a 72kDa protein in red blood cell membrane. HABP possible function in merozoite invasion of erythrocytes is also discussed.",

keywords = "Malaria, Peptides, RAP1",

author = "Hernando Curtidor and Marisol Ocampo and Diana Tovar and Ramses L{\'o}pez and Javier Garc{\'i}a and Jhon Valbuena and Ricardo Vera and Jorge Su{\'a}rez and Rodr{\'i}guez, {Luis E.} and {\'A}lvaro Puentes and Fanny Guzm{\'a}n and Elizabeth Torres and Patarroyo, {Manuel E.}",

year = "2004",

month = feb,

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doi = "10.1016/j.vaccine.2003.07.019",

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Curtidor, H, Ocampo, M, Tovar, D, López, R, García, J, Valbuena, J, Vera, R, Suárez, J, Rodríguez, LE, Puentes, Á, Guzmán, F, Torres, E & Patarroyo, ME 2004, 'Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides', Vaccine, vol. 22, n.º 8, pp. 1054-1062. https://doi.org/10.1016/j.vaccine.2003.07.019

TY - JOUR

T1 - Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides

AU - Curtidor, Hernando

AU - Ocampo, Marisol

AU - Tovar, Diana

AU - López, Ramses

AU - García, Javier

AU - Valbuena, Jhon

AU - Vera, Ricardo

AU - Suárez, Jorge

AU - Rodríguez, Luis E.

AU - Puentes, Álvaro

AU - Guzmán, Fanny

AU - Torres, Elizabeth

AU - Patarroyo, Manuel E.

PY - 2004/2/25

Y1 - 2004/2/25

N2 - Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activity binding peptides (HABPs) were identified; 26201, 26202, 26203 and 26204 spanned residues 461C-K540 within RAP1 Cys region, whilst 26188 (201T-Y220) was located in p67 amino terminal. The results showed that peptide binding was saturable, some HABPs inhibited in vitro merozoite invasion and specifically bound to a 72kDa protein in red blood cell membrane. HABP possible function in merozoite invasion of erythrocytes is also discussed.

AB - Rhoptry-associated protein 1 (RAP1) is a merozoite antigen within Plasmodium falciparum rhoptries as yet having no specific function described for it. Synthetic peptides spanning the RAP1 sequence were tested in erythrocyte binding assays to identify possible RAP1 functional regions. Five high activity binding peptides (HABPs) were identified; 26201, 26202, 26203 and 26204 spanned residues 461C-K540 within RAP1 Cys region, whilst 26188 (201T-Y220) was located in p67 amino terminal. The results showed that peptide binding was saturable, some HABPs inhibited in vitro merozoite invasion and specifically bound to a 72kDa protein in red blood cell membrane. HABP possible function in merozoite invasion of erythrocytes is also discussed.

KW - Malaria

KW - Peptides

KW - RAP1

UR - http://www.scopus.com/inward/record.url?scp=10744226949&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2003.07.019

DO - 10.1016/j.vaccine.2003.07.019

M3 - Article

C2 - 15161083

AN - SCOPUS:10744226949

SN - 0264-410X

VL - 22

SP - 1054

EP - 1062

JO - Vaccine

JF - Vaccine

IS - 8

ER -

Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum-derived rhoptry-associated protein 1 peptides

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