TY - JOUR
T1 - Rabbit serum against K1 peptide, an immunogenic epitope of the Trypanosoma cruzi KMP-11, decreases parasite invasion to cells
AU - Diaz-Soto, Juan Camilo
AU - Lasso, Paola
AU - Guzmán, Fanny
AU - Forero-Shelton, Manu
AU - Thomas, Maria del Carmen
AU - López, Manuel Carlos
AU - Guhl, Felipe
AU - Cuellar, Adriana
AU - Puerta, Concepción Judith
AU - González, John M.
N1 - Funding Information:
This project was funded by an associate professor grant (JMG) from the Vicerrectoria de Investigaciones, Universidad de los Andes; and also received financial support from VRA, Pontificia Universidad Javeriana project No. 003333. PL was supported by COLCIENCIAS project No. 640-2009; and MCT and MCL were supported by grants No. P08-CVI-04037 (Programa PAI de la Junta de Andalucía) and No. RD06/0021/0014 (Programa RETIC del ISCIII- MICINN). The sponsors did not have any participation in the study design, data analysis or paper writing.
PY - 2012/9
Y1 - 2012/9
N2 - KMP-11 is a highly conserved protein of . Trypanosoma cruzi implicated in parasite's motility. Here we show that K1, a peptide derived from KMP-11, induced polyclonal antibodies capable of decreasing . T. cruzi infection in vitro. Rabbit sera rose against K1 peptide showed recognition of the recombinant protein by ELISA and Western blot and also of the native protein in both epimastigotes and trypomastigotes as evaluated by immunofluorescence test and flow cytometry. Invasion assays showed a significant reduction of trypomastigotes infection of eukaryotic cells when parasites were pre-incubated with anti-K1 rabbit serum. Computational modeling predicted that the K1 sequence conserved its α-helical configuration into the protein, and some of the amino acid residues appear accessible for recognition by antibodies in vivo. Taken together, these results support the idea that the K1 peptide induces antibodies than can have a potential role in protective immunity in Chagas disease.
AB - KMP-11 is a highly conserved protein of . Trypanosoma cruzi implicated in parasite's motility. Here we show that K1, a peptide derived from KMP-11, induced polyclonal antibodies capable of decreasing . T. cruzi infection in vitro. Rabbit sera rose against K1 peptide showed recognition of the recombinant protein by ELISA and Western blot and also of the native protein in both epimastigotes and trypomastigotes as evaluated by immunofluorescence test and flow cytometry. Invasion assays showed a significant reduction of trypomastigotes infection of eukaryotic cells when parasites were pre-incubated with anti-K1 rabbit serum. Computational modeling predicted that the K1 sequence conserved its α-helical configuration into the protein, and some of the amino acid residues appear accessible for recognition by antibodies in vivo. Taken together, these results support the idea that the K1 peptide induces antibodies than can have a potential role in protective immunity in Chagas disease.
KW - Anti-peptide antibodies
KW - Chagas disease
KW - Trypanosoma cruzi
UR - http://www.scopus.com/inward/record.url?scp=84863465339&partnerID=8YFLogxK
U2 - 10.1016/j.actatropica.2012.05.015
DO - 10.1016/j.actatropica.2012.05.015
M3 - Article
C2 - 22687575
AN - SCOPUS:84863465339
SN - 0001-706X
VL - 123
SP - 224
EP - 229
JO - Acta Tropica
JF - Acta Tropica
IS - 3
ER -
