Protective in vitro activity of Physalis peruviana calyces extract on oxidation and glycation

Previous studies have demonstrated that Physalis peruviana has antioxidant, antiinflammatory, antidiabetic, and hepatic protective effects. The present work aimed to perform different assays to get a deeper insight into the antioxidant activity of the hydroethanolic extract of calyces from P. peruviana (HEPP) and its fractions. 2,2-Diphenyl-1-picrylhydrazyl (DPPH·) scavenging, lipid peroxidation, low-density lipoprotein cholesterol (LDL-c), and advanced products of glycation (AGEs) were measured. Results showed that the HEPP and its butanol fraction (BFPP) displayed DPPH· radical scavenging activities with IC50 values of 29.9 and 51.4µg/mL, respectively. Similar results were obtained when the diene formation was induced in LDL-c: IC50 values of 211.2 and 103.0µg/mL, for HEPP and BFPP, respectively. HEPP, BFPP and EFPP showed antiglycation activity with IC50 of 141.1, 183.0, and 226.7µg/mL, respectively. In sum, HEPP and BFPP have the potential to prevent tissues from free radicals and thereby protect the cellular membranes from lipid peroxidation.