Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Javier E. García; Alvaro Puentes; Ramsés López; Ricardo Vera; Jorge Suárez; Luis Rodríguez; Hernando Curtidor; Marisol Ocampo; Diana Tovar; Martha Forero; Adriana Bermudez; Jimena Cortés; Mauricio Urquiza; Manuel E. Patarroyo

doi:10.1016/S0196-9781(03)00135-9

Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Javier E. García, Alvaro Puentes, Ramsés López, Ricardo Vera, Jorge Suárez, Luis Rodríguez, Hernando Curtidor, Marisol Ocampo, Diana Tovar, Martha Forero, Adriana Bermudez, Jimena Cortés, Mauricio Urquiza, Manuel E. Patarroyo

Universidad Nacional de Colombia

Producción: Contribución a una revista › Artículo › revisión exhaustiva

17 Citas (Scopus)

Resumen

Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (²¹INGKIIKNSEKDEIIKSNLRY⁴⁰), 20637 (¹⁵⁷KEKLQGQQSDSEQERRAY¹⁷³), 20638 (¹⁷⁴KEKLQEQQSDLEQERLAY¹⁹⁰) and 20639 (¹⁹¹KEKLQEQQSDLEQERRAY²⁰⁷) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (²¹INGKIIKNSEKDEIIKSNLRY⁴⁰) and 20633 (⁸¹DKELTMSNVKNVSQTNFKSLY¹⁰⁰) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

Idioma original	Inglés
Páginas (desde-hasta)	647-657
Número de páginas	11
Publicación	Peptides
Volumen	24
N.º	5
DOI	https://doi.org/10.1016/S0196-9781(03)00135-9
Estado	Publicada - 01 may. 2003
Publicado de forma externa	Sí

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10.1016/S0196-9781(03)00135-9

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@article{1d0aae9c9c814ee9ac762482ff93e4b1,

title = "Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes",

abstract = "Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40), 20637 (157KEKLQGQQSDSEQERRAY173), 20638 (174KEKLQEQQSDLEQERLAY190) and 20639 (191KEKLQEQQSDLEQERRAY207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40) and 20633 (81DKELTMSNVKNVSQTNFKSLY100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 {"}High Activity Binding Peptides{"} could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.",

keywords = "Critical residues, High activity binding peptides (HABPs), Liver stage antigen (LSA), P. falciparum, Phosphate buffered saline (PBS), Red blood cells (RBC)",

author = "Garc{\'i}a, {Javier E.} and Alvaro Puentes and Rams{\'e}s L{\'o}pez and Ricardo Vera and Jorge Su{\'a}rez and Luis Rodr{\'i}guez and Hernando Curtidor and Marisol Ocampo and Diana Tovar and Martha Forero and Adriana Bermudez and Jimena Cort{\'e}s and Mauricio Urquiza and Patarroyo, {Manuel E.}",

year = "2003",

month = may,

day = "1",

doi = "10.1016/S0196-9781(03)00135-9",

language = "English",

volume = "24",

pages = "647--657",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "5",

}

TY - JOUR

T1 - Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

AU - García, Javier E.

AU - Puentes, Alvaro

AU - López, Ramsés

AU - Vera, Ricardo

AU - Suárez, Jorge

AU - Rodríguez, Luis

AU - Curtidor, Hernando

AU - Ocampo, Marisol

AU - Tovar, Diana

AU - Forero, Martha

AU - Bermudez, Adriana

AU - Cortés, Jimena

AU - Urquiza, Mauricio

AU - Patarroyo, Manuel E.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40), 20637 (157KEKLQGQQSDSEQERRAY173), 20638 (174KEKLQEQQSDLEQERLAY190) and 20639 (191KEKLQEQQSDLEQERRAY207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40) and 20633 (81DKELTMSNVKNVSQTNFKSLY100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

AB - Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40), 20637 (157KEKLQGQQSDSEQERRAY173), 20638 (174KEKLQEQQSDLEQERLAY190) and 20639 (191KEKLQEQQSDLEQERRAY207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40) and 20633 (81DKELTMSNVKNVSQTNFKSLY100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

KW - Critical residues

KW - High activity binding peptides (HABPs)

KW - Liver stage antigen (LSA)

KW - P. falciparum

KW - Phosphate buffered saline (PBS)

KW - Red blood cells (RBC)

UR - http://www.scopus.com/inward/record.url?scp=12444344702&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(03)00135-9

DO - 10.1016/S0196-9781(03)00135-9

M3 - Article

C2 - 12895649

AN - SCOPUS:12444344702

SN - 0196-9781

VL - 24

SP - 647

EP - 657

JO - Peptides

JF - Peptides

IS - 5

ER -

Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

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