TY - JOUR
T1 - Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines
AU - García, Javier
AU - Puentes, Alvaro
AU - Rodríguez, Luis
AU - Ocampo, Marisol
AU - Curtidor, Hernando
AU - Vera, Ricardo
AU - Lopez, Ramses
AU - Valbuena, John
AU - Cortes, Jimena
AU - Vanegas, Magnolia
AU - Barrero, Carlos
AU - Patarroyo, Manuel A.
AU - Urquiza, Mauricio
AU - Patarroyo, Manuel E.
PY - 2005/9
Y1 - 2005/9
N2 - The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT-PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25-kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20-amino-acid-long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY 180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross-linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.
AB - The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT-PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25-kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20-amino-acid-long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY 180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross-linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins.
KW - High activity binding peptides
KW - Mycobacterium tuberculosis
KW - Mycobacterium tuberculosis complex
KW - Receptor-ligand interaction
UR - http://www.scopus.com/inward/record.url?scp=24344462098&partnerID=8YFLogxK
U2 - 10.1110/ps.051526305
DO - 10.1110/ps.051526305
M3 - Article
C2 - 16131654
AN - SCOPUS:24344462098
SN - 0961-8368
VL - 14
SP - 2236
EP - 2245
JO - Protein Science
JF - Protein Science
IS - 9
ER -
