Morphological, biochemical, and molecular diagnostics of Meloidogyne spp. associated with Musa spp. In Colombia

D. H. Riascos; A. T. Mosquera-Espinosa; F. Varón de Agudelo; J. M.O. Rosa; C. M.G. Oliveira; J. E. Muñoz

Morphological, biochemical, and molecular diagnostics of Meloidogyne spp. associated with Musa spp. In Colombia

Título traducido de la contribución: Diagnóstico morfológico, bioquímico y molecular de Meloidogyne spp. asociado con Musa spp. en Colombia

D. H. Riascos, A. T. Mosquera-Espinosa, F. Varón de Agudelo, J. M.O. Rosa, C. M.G. Oliveira, J. E. Muñoz

Producción: Contribución a una revista › Artículo › revisión exhaustiva

2 Citas (Scopus)

Resumen

Meloidogyne está entre los nematodos parásitos de plantas que más limita la producción de Musa spp. en el mundo. Para un control eficiente de poblaciones de Meloidogyne, el presente estudio tuvo como propósito identificar especies de este nematodo en cultivos de plátano (Musa AAB Simmonds) y de banano (Musa acuminata AAA) establecidos en diferentes zonas de producción de los departamentos de Valle del Cauca, Quindío, Risaralda y Caldas, Colombia, através de diagnóstico morfológico, bioquímico y molecular. Para tal fin, diferentes poblaciones de Meloidogyne fueron multiplicadas en plántulas de tomate (Solanum lycopersicum cv. Santa Clara) para su identificación basado en la configuración de los patrones perineales y fenotipos de esterasa. La identificación molecular se realizó por amplificación y secuenciación del segmento de expansión D2-D3 del 28S del ARN ribosomal, la región parcial de los genes citocromo oxidasa subunidad I (COI) y NADH dehidrogenasa subunidad 5 (Nad5) del ADN mitocondrial. Se realizaron análisis filogenéticos con los métodos de máxima verosimilitud e inferencia bayesiana para los tres loci. Los resultados mostraron que M. incognita, M. acrita M. arenaria y M. hispanica están asociados a Musaceae en el área de estudio. Este es el primer reporte de M. acrita y M. hispanica en plátano para Colombia.

Título traducido de la contribución	Diagnóstico morfológico, bioquímico y molecular de Meloidogyne spp. asociado con Musa spp. en Colombia
Idioma original	Inglés
Páginas (desde-hasta)	229-245
Número de páginas	17
Publicación	Nematropica
Volumen	49
N.º	2
Estado	Publicada - 2019

Palabras clave

banano
Meloidogyne acrita
Meloidogyne arenaria
Meloidogyne hispanica
Meloidogyne incognita
plátano

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https://journals.flvc.org/nematropica/article/view/120391

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@article{eb1f4ea1a101404f9caf30e5cfa8b46f,

title = "Morphological, biochemical, and molecular diagnostics of Meloidogyne spp. associated with Musa spp. In Colombia",

abstract = "Root-knot nematodes (Meloidogyne spp.) are one of the most destructive plant-parasitic nematodes of Musa spp. around the world. The objective of this study was to identify species of Meloidogyne associated with plantain (Musa AAB Simmonds) and banana (Musa acuminata AAA) crops from Colombia in different zones of Valle del Cauca, Quind{\'i}o, Risaralda, and Caldas using morphological, biochemical, and molecular diagnostics. Each population of Meloidogyne was cultured on tomato (Solanum lycopersicum cv. Santa Clara). Nematodes were then extracted from cultures and species identification was conducted using perineal patterns and esterase phenotypes. Molecular identification was performed by amplifying and sequencing the D2-D3 expansion segments of the 28S nuclear ribosomal RNA gene as well as partial region of cytochrome oxidase subunit I (COI), and NADH dehydrogenase subunit 5 (Nad5) genes of mitochondrial DNA. Phylogenetic analysis were performed using maximum likelihood estimation and Bayesian inference for the three target regions. Results confirmed M. incognita, M. acrita, M. arenaria, and M. hispanica are associated with Musa spp. This is the first report of M. acrita and M. hispanica attacking Musa spp. of Colombia.",

keywords = "Banana, Meloidogyne acrita, Meloidogyne arenaria, Meloidogyne hispanica, Meloidogyne incognita, Plantain, banano, Meloidogyne acrita, Meloidogyne arenaria, Meloidogyne hispanica, Meloidogyne incognita, pl{\'a}tano",

author = "Riascos, {D. H.} and Mosquera-Espinosa, {A. T.} and {Var{\'o}n de Agudelo}, F. and Rosa, {J. M.O.} and Oliveira, {C. M.G.} and Mu{\~n}oz, {J. E.}",

note = "Funding Information: We express our thanks to Colciencias (Departamento Administrativo de Ciencias, Tecnolog{\'i}a e Innovaci{\'o}n) for the financial support of the first author for doctoral studies, his internship, and the research (announcement 727-2015). We thank the project “Tecnolog{\'i}as innovadoras para el Manejo Integrado de plagas y enfermedades limitantes de pl{\'a}tano y banana en el Valle del Cauca” from the Sistema General de Regal{\'i}as-SGR of Colombia for financial support of the collection of roots and soil samples in different farms and the acquisition of reagents. We thank the Molecular Biology Laboratory of the Universidad Nacional de Colombia in Palmira, Valle del Cauca-Colombia and Nematology Laboratory of Instituto Biol{\'o}gico in Campinas, S{\~a}o Paulo-Brazil for scientific support for the collecting molecular, morphological, morphometric and biochemical data. We thank the Universidad del Pacif{\'i}co in Buenaventura, Colombia. We thank Peter Ramley for his contributions toward editing the manuscript. We thank Drs. Angel Vale and Danny Rojas for their support with the phylogenetic analyses and manuscript revision. We thank CIPRES for support with bioinformatics. Publisher Copyright: {\textcopyright} 2019 Organization of Nematologists of Tropical America. All rights reserved.",

year = "2019",

language = "English",

volume = "49",

pages = "229--245",

journal = "Nematropica",

issn = "0099-5444",

publisher = "Organization of Nematologists of Tropical America",

number = "2",

}

TY - JOUR

T1 - Morphological, biochemical, and molecular diagnostics of Meloidogyne spp. associated with Musa spp. In Colombia

AU - Riascos, D. H.

AU - Mosquera-Espinosa, A. T.

AU - Varón de Agudelo, F.

AU - Rosa, J. M.O.

AU - Oliveira, C. M.G.

AU - Muñoz, J. E.

N1 - Funding Information: We express our thanks to Colciencias (Departamento Administrativo de Ciencias, Tecnología e Innovación) for the financial support of the first author for doctoral studies, his internship, and the research (announcement 727-2015). We thank the project “Tecnologías innovadoras para el Manejo Integrado de plagas y enfermedades limitantes de plátano y banana en el Valle del Cauca” from the Sistema General de Regalías-SGR of Colombia for financial support of the collection of roots and soil samples in different farms and the acquisition of reagents. We thank the Molecular Biology Laboratory of the Universidad Nacional de Colombia in Palmira, Valle del Cauca-Colombia and Nematology Laboratory of Instituto Biológico in Campinas, São Paulo-Brazil for scientific support for the collecting molecular, morphological, morphometric and biochemical data. We thank the Universidad del Pacifíco in Buenaventura, Colombia. We thank Peter Ramley for his contributions toward editing the manuscript. We thank Drs. Angel Vale and Danny Rojas for their support with the phylogenetic analyses and manuscript revision. We thank CIPRES for support with bioinformatics. Publisher Copyright: © 2019 Organization of Nematologists of Tropical America. All rights reserved.

PY - 2019

Y1 - 2019

N2 - Root-knot nematodes (Meloidogyne spp.) are one of the most destructive plant-parasitic nematodes of Musa spp. around the world. The objective of this study was to identify species of Meloidogyne associated with plantain (Musa AAB Simmonds) and banana (Musa acuminata AAA) crops from Colombia in different zones of Valle del Cauca, Quindío, Risaralda, and Caldas using morphological, biochemical, and molecular diagnostics. Each population of Meloidogyne was cultured on tomato (Solanum lycopersicum cv. Santa Clara). Nematodes were then extracted from cultures and species identification was conducted using perineal patterns and esterase phenotypes. Molecular identification was performed by amplifying and sequencing the D2-D3 expansion segments of the 28S nuclear ribosomal RNA gene as well as partial region of cytochrome oxidase subunit I (COI), and NADH dehydrogenase subunit 5 (Nad5) genes of mitochondrial DNA. Phylogenetic analysis were performed using maximum likelihood estimation and Bayesian inference for the three target regions. Results confirmed M. incognita, M. acrita, M. arenaria, and M. hispanica are associated with Musa spp. This is the first report of M. acrita and M. hispanica attacking Musa spp. of Colombia.

AB - Root-knot nematodes (Meloidogyne spp.) are one of the most destructive plant-parasitic nematodes of Musa spp. around the world. The objective of this study was to identify species of Meloidogyne associated with plantain (Musa AAB Simmonds) and banana (Musa acuminata AAA) crops from Colombia in different zones of Valle del Cauca, Quindío, Risaralda, and Caldas using morphological, biochemical, and molecular diagnostics. Each population of Meloidogyne was cultured on tomato (Solanum lycopersicum cv. Santa Clara). Nematodes were then extracted from cultures and species identification was conducted using perineal patterns and esterase phenotypes. Molecular identification was performed by amplifying and sequencing the D2-D3 expansion segments of the 28S nuclear ribosomal RNA gene as well as partial region of cytochrome oxidase subunit I (COI), and NADH dehydrogenase subunit 5 (Nad5) genes of mitochondrial DNA. Phylogenetic analysis were performed using maximum likelihood estimation and Bayesian inference for the three target regions. Results confirmed M. incognita, M. acrita, M. arenaria, and M. hispanica are associated with Musa spp. This is the first report of M. acrita and M. hispanica attacking Musa spp. of Colombia.

KW - Banana

KW - Meloidogyne acrita

KW - Meloidogyne arenaria

KW - Meloidogyne hispanica

KW - Meloidogyne incognita

KW - Plantain

KW - banano

KW - Meloidogyne acrita

KW - Meloidogyne arenaria

KW - Meloidogyne hispanica

KW - Meloidogyne incognita

KW - plátano

UR - https://journals.flvc.org/nematropica/article/view/120391

M3 - Article

AN - SCOPUS:85091672175

SN - 0099-5444

VL - 49

SP - 229

EP - 245

JO - Nematropica

JF - Nematropica

IS - 2

ER -

Morphological, biochemical, and molecular diagnostics of Meloidogyne spp. associated with Musa spp. In Colombia

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