TY - JOUR
T1 - Monoaminergic neurotransmission is mediating the antidepressant-like effects of Passiflora edulis Sims fo. edulis
AU - Ayres, Adriana S.F.S.J.
AU - Santos, Wilton B.
AU - Junqueira-Ayres, Décio D.
AU - Costa, Geison M.
AU - Ramos, Freddy A.
AU - Castellanos, Leonardo
AU - Alves, Jovelina S.F.
AU - Asth, Laila
AU - Medeiros, Iris U.de
AU - Zucolotto, Silvana M.
AU - Gavioli, Elaine C.
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - The genus Passiflora is popularly used to treat anxiety. Recent studies showed antidepressant-like effects of two varieties of P. edulis (edulis and flavicarpa) in mice. However, the mechanisms of antidepressant actions are still unknown. Here, the effects of P. edulis fo. edulis aqueous extract (AE, 100–300 mg/kg, po), and ethyl acetate (AcOEt, 25–50 mg/kg, po), butanol (BuOH, 25–50 mg/kg, po) and residual aqueous (25–100 mg/kg, po) fractions were investigated in the mouse forced swimming test. In addition, the involvement of monoamines in the P. edulis fractions-induced antidepressant actions was approached. HPLC analyses showed that AcOEt and BuOH, but not residual, fractions shared with AE the main peaks between 25 and 70 min (UV 340 nm), which are suggestive of flavonoids. Nortriptyline and fluoxetine reduced the immobility time and similar results were observed for AE, AcOEt and BuOH but not residual fractions. PCPA (inhibitor of 5-HT synthesis), AMPT (inhibitor of catecholamine synthesis) and sulpiride (selective D2 receptor antagonist), but not DSP-4 (noradrenergic neurotoxin), blocked the antidepressant actions of AcOEt and BuOH. In conclusion, AcOEt and BuOH fractions shared with AE similar phytochemical composition and antidepressant actions. Preserved 5-HT and dopamine transmissions were required for the antidepressant effects of P. edulis fractions.
AB - The genus Passiflora is popularly used to treat anxiety. Recent studies showed antidepressant-like effects of two varieties of P. edulis (edulis and flavicarpa) in mice. However, the mechanisms of antidepressant actions are still unknown. Here, the effects of P. edulis fo. edulis aqueous extract (AE, 100–300 mg/kg, po), and ethyl acetate (AcOEt, 25–50 mg/kg, po), butanol (BuOH, 25–50 mg/kg, po) and residual aqueous (25–100 mg/kg, po) fractions were investigated in the mouse forced swimming test. In addition, the involvement of monoamines in the P. edulis fractions-induced antidepressant actions was approached. HPLC analyses showed that AcOEt and BuOH, but not residual, fractions shared with AE the main peaks between 25 and 70 min (UV 340 nm), which are suggestive of flavonoids. Nortriptyline and fluoxetine reduced the immobility time and similar results were observed for AE, AcOEt and BuOH but not residual fractions. PCPA (inhibitor of 5-HT synthesis), AMPT (inhibitor of catecholamine synthesis) and sulpiride (selective D2 receptor antagonist), but not DSP-4 (noradrenergic neurotoxin), blocked the antidepressant actions of AcOEt and BuOH. In conclusion, AcOEt and BuOH fractions shared with AE similar phytochemical composition and antidepressant actions. Preserved 5-HT and dopamine transmissions were required for the antidepressant effects of P. edulis fractions.
KW - Antidepressants
KW - Dopamine
KW - Forced swimming test
KW - Mouse
KW - Passiflora edulis
KW - Serotonin
UR - http://www.scopus.com/inward/record.url?scp=85029544081&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2017.09.010
DO - 10.1016/j.neulet.2017.09.010
M3 - Article
C2 - 28893593
AN - SCOPUS:85029544081
SN - 0304-3940
VL - 660
SP - 79
EP - 85
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -