Microbiological and Molecular Characteristics of Microorganisms of Importance in Dental Caries and Periodontal Disease: Research Contributions in Colombia

Dabeiba Adriana Garcia Robayo; Fredy Omar Gamboa Jaimes; Leandro Augusto Plazas Cristancho

doi:10.11144/javeriana.uo39.cmmm

Microbiological and Molecular Characteristics of Microorganisms of Importance in Dental Caries and Periodontal Disease: Research Contributions in Colombia

Título traducido de la contribución: Características microbiológicas y moleculares de microorganismos de importancia en caries dental y enfermedad periodontal: aportes de investigación en Colombia

Dabeiba Adriana Garcia Robayo, Fredy Omar Gamboa Jaimes, Leandro Augusto Plazas Cristancho

Universidad Javeriana

Producción: Contribución a una revista › Artículo › revisión exhaustiva

Resumen

Antecedentes: La caries dental es un proceso patológico infeccioso y multifactorial que destruye los tejidos duros dentales, y Streptococcus mutans es el principal microorganismo asociado a esta enfermedad. La enfermedad periodontal es una enfermedad infecciosa multifactorial y en su etiología Porphyromonas gingivalis es muy importante por sus factores de virulencia y papel en la patología periodontal. Objetivos y metodología: este artículo de revisión integradora construido con base en experiencias de investigación ya publicadas de los autores de este documento y contrastadas con otros estudios a nivel mundial, tuvo como objetivo describir las características microbiológicas y moleculares de S. mutans y P. gingivalis, microorganismos, respectivamente, de gran importancia en caries dental y enfermedad periodontal. Se encontraron importantes estudios con el uso de las siguientes palabras clave o Medical Subject Headings (MeSH) en PubMed, Scopus y Google Scholar: “S. mutans” y “P. gingivalis” y “identificación fenotípica” y “caracterización genotípica”. Resultados: De S. mutans, se identificaron diferentes biotipos de acuerdo a la metodología empleada, amplia susceptibilidad antimicrobiana y variabilidad genotípica por AP-PCR que depende del anidamiento de los primers sobre el ADN. De P. gingivalis se encontró alta susceptibilidad a los antimicrobianos y amplia discriminación genotípica por la técnica AFLP. Conclusión: el profundo conocimiento fenotípico y genotípico de S. mutans y P. gingivalis, bacterias de gran importancia en caries dental y enfermedad periodontal, unido al estudio epidemiológico clásico podría ser muy importante en la aplicación de mejores estrategias de prevención y control que impacten la salud pública oral en estas dos enfermedades.

Título traducido de la contribución	Características microbiológicas y moleculares de microorganismos de importancia en caries dental y enfermedad periodontal: aportes de investigación en Colombia
Idioma original	Inglés
Número de páginas	45
Publicación	Universitas Odontologica
Volumen	39
DOI	https://doi.org/10.11144/javeriana.uo39.cmmm
Estado	Publicada - 29 oct. 2020

Palabras clave

caracterización molecular
estudio fenotípico
microbiología oral
odontología
P. gingivalis
Porphyromonas gingivalis
Streptococcus mutans
S.mutans

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10.11144/javeriana.uo39.cmmm

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http://dx.doi.org/10.11144/javeriana.uo39.cmmm

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@article{eb003026c4ab4cf88d99c76517a6d48a,

title = "Microbiological and Molecular Characteristics of Microorganisms of Importance in Dental Caries and Periodontal Disease: Research Contributions in Colombia",

abstract = "Background: Dental caries is an infectious and multifactorial pathological process that destroys hard dental tissues, and Streptococcus mutans is found as the main microorganism associated with this disease.a multifactorial infectious disease, and, in its etiology, diverse microorganisms are involved, among which Porphyromonas gingivalis is of vital importance due to its virulence factors and role in the development of periodontal pathology. Purpose and Method: This integrative review article built on the basis of previously published research experiences of the authors of this document and contrasted with other studies worldwide, aimed to describe the microbiological and molecular characteristics of S. mutans and P. gingivalis, microorganisms, respectively, of great importance in dental caries and periodontal disease. Important studies were found with the use of the following keywords or medical subject headings (MeSH) in Pubmed, Scopus and Google Scholar: “S. mutans” and “P. gingivalis” and {"}phenotypic identification{"} and {"}genotypic characterization”. Results: From S. mutans, different biotypes were identified according to the methodology used, wide antimicrobial susceptibility and genotypic variability by AP-PCR that depends on the nesting of the primers on the DNA. Of P. gingivalis, high susceptibility to antimicrobials and broad genotypic discrimination were found by the AFLP technique. Conclusion: the deep phenotypic and genotypic knowledge of S. mutans and P. gingivalis, bacteria of great importance in dental caries and periodontal disease, together with the classical epidemiological study could be very important in the application of better prevention and control strategies that impact the oral public health in these two diseases.",

keywords = "caracterizaci{\'o}n molecular, estudio fenot{\'i}pico, microbiolog{\'i}a oral, odontolog{\'i}a, P. gingivalis, Porphyromonas gingivalis, Streptococcus mutans, S.mutans, dentistry, molecular characterization, oral microbiology, P. gingivalis, Porphyromonas gingivalis, Streptococcus mutans, S.mutans",

author = "{Garcia Robayo}, {Dabeiba Adriana} and {Gamboa Jaimes}, {Fredy Omar} and {Plazas Cristancho}, {Leandro Augusto}",

year = "2020",

month = oct,

day = "29",

doi = "10.11144/javeriana.uo39.cmmm",

language = "English",

volume = "39",

journal = "Universitas Odontologica",

issn = "2027-3444",

}

Microbiological and Molecular Characteristics of Microorganisms of Importance in Dental Caries and Periodontal Disease: Research Contributions in Colombia. / Garcia Robayo, Dabeiba Adriana ; Gamboa Jaimes, Fredy Omar; Plazas Cristancho, Leandro Augusto.
En: Universitas Odontologica, Vol. 39, 29.10.2020.

Producción: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Microbiological and Molecular Characteristics of Microorganisms of Importance in Dental Caries and Periodontal Disease

T2 - Research Contributions in Colombia

AU - Garcia Robayo, Dabeiba Adriana

AU - Gamboa Jaimes, Fredy Omar

AU - Plazas Cristancho, Leandro Augusto

PY - 2020/10/29

Y1 - 2020/10/29

N2 - Background: Dental caries is an infectious and multifactorial pathological process that destroys hard dental tissues, and Streptococcus mutans is found as the main microorganism associated with this disease.a multifactorial infectious disease, and, in its etiology, diverse microorganisms are involved, among which Porphyromonas gingivalis is of vital importance due to its virulence factors and role in the development of periodontal pathology. Purpose and Method: This integrative review article built on the basis of previously published research experiences of the authors of this document and contrasted with other studies worldwide, aimed to describe the microbiological and molecular characteristics of S. mutans and P. gingivalis, microorganisms, respectively, of great importance in dental caries and periodontal disease. Important studies were found with the use of the following keywords or medical subject headings (MeSH) in Pubmed, Scopus and Google Scholar: “S. mutans” and “P. gingivalis” and "phenotypic identification" and "genotypic characterization”. Results: From S. mutans, different biotypes were identified according to the methodology used, wide antimicrobial susceptibility and genotypic variability by AP-PCR that depends on the nesting of the primers on the DNA. Of P. gingivalis, high susceptibility to antimicrobials and broad genotypic discrimination were found by the AFLP technique. Conclusion: the deep phenotypic and genotypic knowledge of S. mutans and P. gingivalis, bacteria of great importance in dental caries and periodontal disease, together with the classical epidemiological study could be very important in the application of better prevention and control strategies that impact the oral public health in these two diseases.

AB - Background: Dental caries is an infectious and multifactorial pathological process that destroys hard dental tissues, and Streptococcus mutans is found as the main microorganism associated with this disease.a multifactorial infectious disease, and, in its etiology, diverse microorganisms are involved, among which Porphyromonas gingivalis is of vital importance due to its virulence factors and role in the development of periodontal pathology. Purpose and Method: This integrative review article built on the basis of previously published research experiences of the authors of this document and contrasted with other studies worldwide, aimed to describe the microbiological and molecular characteristics of S. mutans and P. gingivalis, microorganisms, respectively, of great importance in dental caries and periodontal disease. Important studies were found with the use of the following keywords or medical subject headings (MeSH) in Pubmed, Scopus and Google Scholar: “S. mutans” and “P. gingivalis” and "phenotypic identification" and "genotypic characterization”. Results: From S. mutans, different biotypes were identified according to the methodology used, wide antimicrobial susceptibility and genotypic variability by AP-PCR that depends on the nesting of the primers on the DNA. Of P. gingivalis, high susceptibility to antimicrobials and broad genotypic discrimination were found by the AFLP technique. Conclusion: the deep phenotypic and genotypic knowledge of S. mutans and P. gingivalis, bacteria of great importance in dental caries and periodontal disease, together with the classical epidemiological study could be very important in the application of better prevention and control strategies that impact the oral public health in these two diseases.

KW - caracterización molecular

KW - estudio fenotípico

KW - microbiología oral

KW - odontología

KW - P. gingivalis

KW - Porphyromonas gingivalis

KW - Streptococcus mutans

KW - S.mutans

KW - dentistry

KW - molecular characterization

KW - oral microbiology

KW - P. gingivalis

KW - Porphyromonas gingivalis

KW - Streptococcus mutans

KW - S.mutans

UR - http://dx.doi.org/10.11144/javeriana.uo39.cmmm

U2 - 10.11144/javeriana.uo39.cmmm

DO - 10.11144/javeriana.uo39.cmmm

M3 - Article

SN - 2027-3444

VL - 39

JO - Universitas Odontologica

JF - Universitas Odontologica

ER -

Microbiological and Molecular Characteristics of Microorganisms of Importance in Dental Caries and Periodontal Disease: Research Contributions in Colombia

Resumen

Palabras clave

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