Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris

Henry A. Córdoba-Ruiz; Raúl A. Poutou-Piñales; Olga Y. Echeverri-Peña; Néstor A. Algecira-Enciso; Patricia Landázuri; Homero Sáenz; Luis A. Barrera-Avellaneda

Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris

Henry A. Córdoba-Ruiz
, Raúl A. Poutou-Piñales
, Olga Y. Echeverri-Peña
, Néstor A. Algecira-Enciso
, Patricia Landázuri
, Homero Sáenz
, Luis A. Barrera-Avellaneda

Pontificia Universidad Javeriana
Universidad Nacional de Colombia
Universidad del Quindío
Universidad Centroccidental Lisandro Alvarado

Producción: Contribución a una revista › Artículo › revisión exhaustiva

26 Citas (Scopus)

Resumen

Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDS-Like) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. The biological activity found was between 7.3 and 29.5 nmol h ^-1 mg ^-1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same clone in shake flask culture.

Idioma original	Inglés
Páginas (desde-hasta)	1786-1792
Número de páginas	7
Publicación	African Journal of Biotechnology
Volumen	8
N.º	9
Estado	Publicada - 04 may 2009

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title = "Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris",

abstract = "Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDS-Like) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. The biological activity found was between 7.3 and 29.5 nmol h -1 mg -1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same clone in shake flask culture.",

keywords = "Hunter syndrome, Iduronate 2-sulfate sulfatase, Methanol induction, Monod model, Pichia pastoris",

author = "C{\'o}rdoba-Ruiz, \{Henry A.\} and Poutou-Pi{\~n}ales, \{Ra{\'u}l A.\} and Echeverri-Pe{\~n}a, \{Olga Y.\} and Algecira-Enciso, \{N{\'e}stor A.\} and Patricia Land{\'a}zuri and Homero S{\'a}enz and Barrera-Avellaneda, \{Luis A.\}",

year = "2009",

month = may,

day = "4",

language = "English",

volume = "8",

pages = "1786--1792",

journal = "African Journal of Biotechnology",

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T1 - Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris

AU - Córdoba-Ruiz, Henry A.

AU - Poutou-Piñales, Raúl A.

AU - Echeverri-Peña, Olga Y.

AU - Algecira-Enciso, Néstor A.

AU - Landázuri, Patricia

AU - Sáenz, Homero

AU - Barrera-Avellaneda, Luis A.

PY - 2009/5/4

Y1 - 2009/5/4

N2 - Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDS-Like) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. The biological activity found was between 7.3 and 29.5 nmol h -1 mg -1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same clone in shake flask culture.

AB - Clone IDS28 of the yeast Pichia pastoris expressing the human iduronate 2-sulfate sulfatase-Like (hIDS-Like) was employed for low-scale production of the recombinant enzyme in a saline culture media without phosphate. The biological activity found was between 7.3 and 29.5 nmol h -1 mg -1 of total protein. It is about 1.73 to 7 times higher than the result obtained with the same clone in shake flask culture.

KW - Hunter syndrome

KW - Iduronate 2-sulfate sulfatase

KW - Methanol induction

KW - Monod model

KW - Pichia pastoris

UR - https://www.scopus.com/pages/publications/66449098524

M3 - Article

AN - SCOPUS:66449098524

SN - 1684-5315

VL - 8

SP - 1786

EP - 1792

JO - African Journal of Biotechnology

JF - African Journal of Biotechnology

IS - 9

ER -

Laboratory scale production of the human recombinant iduronate 2-sulfate sulfatase-Like from Pichia pastoris

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