Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

J. M. Manterola; F. Gamboa; J. Lonca; L. Matas; J. R. Manzano; C. Rodrigo; V. Ausina

doi:10.1128/jcm.33.12.3338-3340.1995

Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

Título traducido de la contribución: Efecto inhibidor del dodecil sulfato de sodio en la detección de Mycobacterium tuberculosis mediante amplificación de ARNr

J. M. Manterola, F. Gamboa, J. Lonca, L. Matas, J. R. Manzano, C. Rodrigo, V. Ausina

Universidad Autónoma de Barcelona

Producción: Contribución a una revista › Artículo › revisión exhaustiva

10 Citas (Scopus)

Resumen

Las concentraciones de dodecil (lauril) sulfato de sodio (SDS) encontradas en los sedimentos de las muestras respiratorias pretratadas con SDS-NaOH variaron entre 3,36 y 12,42 mg/ml. Estas concentraciones de SDS superaron el nivel considerado crítico (≥0,16 mg/ml) para obtener resultados negativos con la prueba directa de Mycobacterium Tuberculosis con amplificación GenProbe. Un lavado exhaustivo, tras la neutralización del sedimento, es suficiente para eliminar cualquier resto de detergente que interfiera con las enzimas utilizadas en la prueba directa de Mycobacterium Tuberculosis.

Título traducido de la contribución	Efecto inhibidor del dodecil sulfato de sodio en la detección de Mycobacterium tuberculosis mediante amplificación de ARNr
Idioma original	Inglés
Páginas (desde-hasta)	3338-3340
Número de páginas	3
Publicación	Journal of Clinical Microbiology
Volumen	33
N.º	12
DOI	https://doi.org/10.1128/jcm.33.12.3338-3340.1995
Estado	Publicada - 01 dic. 1995
Publicado de forma externa	Sí

Palabras clave

Bacterias (microorganismos)
Mycobacterium
Mycobacterium tuberculosis

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title = "Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA",

abstract = "The concentrations of sodium dodecyl (lauryl) sulfate (SDS) found in the sediments of the respiratory samples pretreated with SDS-NaOH varied between 3.36 and 12.42 mg/ml. These concentrations of SDS were higher than the level considered critical (> or = 0.16 mg/ml) to obtain negative results with the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Extensive washing, after neutralization of sediment, is sufficient to remove all traces of detergent which interfere with the enzymes used by the Mycobacterium Tuberculosis Direct Test assay.",

keywords = "Bacteria (microorganisms), Mycobacterium, Mycobacterium tuberculosis, Bacterias (microorganismos), Mycobacterium, Mycobacterium tuberculosis",

author = "Manterola, \{J. M.\} and F. Gamboa and J. Lonca and L. Matas and Manzano, \{J. R.\} and C. Rodrigo and V. Ausina",

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T1 - Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

AU - Manterola, J. M.

AU - Gamboa, F.

AU - Lonca, J.

AU - Matas, L.

AU - Manzano, J. R.

AU - Rodrigo, C.

AU - Ausina, V.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - The concentrations of sodium dodecyl (lauryl) sulfate (SDS) found in the sediments of the respiratory samples pretreated with SDS-NaOH varied between 3.36 and 12.42 mg/ml. These concentrations of SDS were higher than the level considered critical (> or = 0.16 mg/ml) to obtain negative results with the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Extensive washing, after neutralization of sediment, is sufficient to remove all traces of detergent which interfere with the enzymes used by the Mycobacterium Tuberculosis Direct Test assay.

AB - The concentrations of sodium dodecyl (lauryl) sulfate (SDS) found in the sediments of the respiratory samples pretreated with SDS-NaOH varied between 3.36 and 12.42 mg/ml. These concentrations of SDS were higher than the level considered critical (> or = 0.16 mg/ml) to obtain negative results with the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Extensive washing, after neutralization of sediment, is sufficient to remove all traces of detergent which interfere with the enzymes used by the Mycobacterium Tuberculosis Direct Test assay.

KW - Bacteria (microorganisms)

KW - Mycobacterium

KW - Mycobacterium tuberculosis

KW - Bacterias (microorganismos)

KW - Mycobacterium

KW - Mycobacterium tuberculosis

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DO - 10.1128/jcm.33.12.3338-3340.1995

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Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

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