TY - JOUR
T1 - Increased calcium influx triggers and accelerates cortical spreading depression in vivo in male adult rats
AU - Torrente, Daniel
AU - Mendes-da-Silva, Rosângela Figueiredo
AU - Lopes, Andréia Albuquerque Cunha
AU - González, Janneth
AU - Barreto, George E.
AU - Guedes, Rubem Carlos Araújo
N1 - Funding Information:
This work was supported in part by PUJ grants IDs 5024 and 4367 , and PROLAB IBRO/LARC CNPq – Convenio Brazil/Colombia to GEB, and by CNPq (INCT de Neurociência Translacional ; No. 573604/2008-8 ); Edital MCT/CNPq 14/2010 (No. 477456/2010-3 ) and IBN-Net/Finep (No. 4191 ) to RCAG.
PY - 2014/1/13
Y1 - 2014/1/13
N2 - Cortical spreading depression (CSD) is a depolarization wave associated with neurological disorders such as migraine, cerebral ischemia and traumatic brain injury. The mechanism of action of this phenomenon still remains unclear. Although it is suggested that extracellular K+ accumulation contributes to CSD, other ions may play a relevant role in the mechanism of propagation of the wave. In this context, we hypothesize that Ca2+ may play an important function in the wave propagation. Our results demonstrate that enhancing Ca2+ influx into the cells by topical cortical application of the ionophore A23187 (10μM, 50μM and 100μM solutions) increases the velocity of CSD propagation in a dose-dependent manner, and a much higher dose of this compound (2mM) triggers CSD. In conclusion, increased Ca2+ influx can be a key element in the induction mechanism of the CSD, and should be assessed in further experimental strategies targeting brain disorders related to CSD.
AB - Cortical spreading depression (CSD) is a depolarization wave associated with neurological disorders such as migraine, cerebral ischemia and traumatic brain injury. The mechanism of action of this phenomenon still remains unclear. Although it is suggested that extracellular K+ accumulation contributes to CSD, other ions may play a relevant role in the mechanism of propagation of the wave. In this context, we hypothesize that Ca2+ may play an important function in the wave propagation. Our results demonstrate that enhancing Ca2+ influx into the cells by topical cortical application of the ionophore A23187 (10μM, 50μM and 100μM solutions) increases the velocity of CSD propagation in a dose-dependent manner, and a much higher dose of this compound (2mM) triggers CSD. In conclusion, increased Ca2+ influx can be a key element in the induction mechanism of the CSD, and should be assessed in further experimental strategies targeting brain disorders related to CSD.
KW - Ca influx
KW - Cortical spreading depression
KW - Ionophore
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=84889578708&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2013.11.004
DO - 10.1016/j.neulet.2013.11.004
M3 - Article
C2 - 24246901
AN - SCOPUS:84889578708
SN - 0304-3940
VL - 558
SP - 87
EP - 90
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -