TY - JOUR
T1 - In vitro antiviral activity against rotavirus and astrovirus infection exerted by substances obtained from Achyrocline bogotensis (Kunth) DC. (Compositae)
AU - Téllez Corral, Mayra Alexandra
AU - Ulloa Rubiano, Juan Carlos
AU - Tellez Alfonso, Alba Nohemí
AU - Vélez, F.
N1 - Publisher Copyright:
© 2015 Téllez et al.
PY - 2015/12/3
Y1 - 2015/12/3
N2 - MethodsSeveral extracts and fractions phytochemically obtained from A. bogotensis were evaluated initially for their cell toxicity on MA104 and Caco2 cells and then for their anti-rotavirus (RRV) and anti-astrovirus (Yuc8) activity following three strategies: pre-treatment of cells (blocking effect), direct viral activity (virucidal effect) and post-treatment of infected cells (reduction of viral yield post-infection). In addition qualitative chemical studies were developed for the active compounds.
ResultsNon-toxic concentrations of a fraction obtained exhibited antiviral activity against both viruses characterized by a virucidal effect and by the reduction of the infectious particles produced post-infection. Steroids, sterols, terpenes, phenols, flavonoids and sesquiterpenlactones were identified qualitatively in the active fraction.
ConclusionsA. bogotensis contains substances with in vitro antiviral activity against rotavirus and astrovirus. This study confirms their anti-microbial properties and describes by the first time its antiviral activity in vitro.
AB - MethodsSeveral extracts and fractions phytochemically obtained from A. bogotensis were evaluated initially for their cell toxicity on MA104 and Caco2 cells and then for their anti-rotavirus (RRV) and anti-astrovirus (Yuc8) activity following three strategies: pre-treatment of cells (blocking effect), direct viral activity (virucidal effect) and post-treatment of infected cells (reduction of viral yield post-infection). In addition qualitative chemical studies were developed for the active compounds.
ResultsNon-toxic concentrations of a fraction obtained exhibited antiviral activity against both viruses characterized by a virucidal effect and by the reduction of the infectious particles produced post-infection. Steroids, sterols, terpenes, phenols, flavonoids and sesquiterpenlactones were identified qualitatively in the active fraction.
ConclusionsA. bogotensis contains substances with in vitro antiviral activity against rotavirus and astrovirus. This study confirms their anti-microbial properties and describes by the first time its antiviral activity in vitro.
KW - Antiviral
KW - Rotavirus
KW - Astrovirus
KW - Achyrocline bogotensis
UR - https://www.scopus.com/pages/publications/84949235344
U2 - 10.1186/s12906-015-0949-0
DO - 10.1186/s12906-015-0949-0
M3 - Article
C2 - 26630872
AN - SCOPUS:84949235344
SN - 1472-6882
VL - 15
SP - 1
EP - 10
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
IS - 428
M1 - 428
ER -