Immunological and structural characterization of an epitope from the Trypanosoma cruzi KMP-11 protein

H. Díez; F. Guzmán; M. P. Alba; A. Cuéllar; M. C. Thomas; M. C. López; F. Rosas; V. Velasco; J. M. González; M. E. Patarroyo; C. J. Puerta

doi:10.1016/j.peptides.2007.06.017

Immunological and structural characterization of an epitope from the Trypanosoma cruzi KMP-11 protein

H. Díez
, F. Guzmán
, M. P. Alba
, A. Cuéllar
, M. C. Thomas
, M. C. López
, F. Rosas
, V. Velasco
, J. M. González
, M. E. Patarroyo
, C. J. Puerta

Departamento de Microbiología

Fundación Instituto de Inmunología de Colombia
Consejo Sup. de Investigacions Cie.
Diag
Universidad de los Andes Colombia

Producción: Contribución a una revista › Artículo › revisión exhaustiva

14 Citas (Scopus)

Resumen

The K1 peptide is an HLA-A*0201-restricted cytotoxic epitope derived from the Trypanosoma cruzi KMP-11 protein, this being the etiological agent of Chagas' disease. This work describes the K1 peptide's secondary structure and its recognition by sera from chagasic patients. Circular dichroism and NMR spectroscopy analysis revealed that the K1 peptide adopts an α-helical conformation. Fifty-six percent of individuals had anti-K1 and 86% anti-KMP-11 antibodies by ELISA in the chronic Chagas' group and 28 and 68% in the indeterminate Chagas' group, respectively. By contrast, no reactivity was observed in sera from healthy individuals and tuberculosis patients. Antibody response subclass specificity to the K1 peptide was IgG1 and IgG3. Taken together these results support the idea that the K1 peptide acts as a B-cell-inducer epitope during Chagas' disease.

Idioma original	Inglés
Páginas (desde-hasta)	1520-1526
Número de páginas	7
Publicación	Peptides
Volumen	28
N.º	8
DOI	https://doi.org/10.1016/j.peptides.2007.06.017
Estado	Publicada - ago 2007

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@article{e08e7bf6f8944fb7a89a30841e6fba31,

title = "Immunological and structural characterization of an epitope from the Trypanosoma cruzi KMP-11 protein",

abstract = "The K1 peptide is an HLA-A*0201-restricted cytotoxic epitope derived from the Trypanosoma cruzi KMP-11 protein, this being the etiological agent of Chagas' disease. This work describes the K1 peptide's secondary structure and its recognition by sera from chagasic patients. Circular dichroism and NMR spectroscopy analysis revealed that the K1 peptide adopts an α-helical conformation. Fifty-six percent of individuals had anti-K1 and 86\% anti-KMP-11 antibodies by ELISA in the chronic Chagas' group and 28 and 68\% in the indeterminate Chagas' group, respectively. By contrast, no reactivity was observed in sera from healthy individuals and tuberculosis patients. Antibody response subclass specificity to the K1 peptide was IgG1 and IgG3. Taken together these results support the idea that the K1 peptide acts as a B-cell-inducer epitope during Chagas' disease.",

keywords = "Chagas' disease, Humoral immune response, K1 peptide, KMP-11 protein, Trypanosoma cruzi, α-Helix",

author = "H. D{\'i}ez and F. Guzm{\'a}n and Alba, \{M. P.\} and A. Cu{\'e}llar and Thomas, \{M. C.\} and L{\'o}pez, \{M. C.\} and F. Rosas and V. Velasco and Gonz{\'a}lez, \{J. M.\} and Patarroyo, \{M. E.\} and Puerta, \{C. J.\}",

note = "Funding Information: The authors wish to thank Marcela Mercado from Universidad Javeriana de Bogot{\'a}, Colombia for the statistical analysis and to Adriana Rodriguez from Universidad Militar Nueva Granada for supporting ELISA techniques. M.C. Thomas and M.C. Lopez were supported by P06-CTS-02242 and RD06/0021/0014 grants from PAI and FIS, Spain. FIDIC was supported by COLCIENCIAS Contract No. RC-2007.",

year = "2007",

month = aug,

doi = "10.1016/j.peptides.2007.06.017",

language = "English",

volume = "28",

pages = "1520--1526",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "8",

}

TY - JOUR

T1 - Immunological and structural characterization of an epitope from the Trypanosoma cruzi KMP-11 protein

AU - Díez, H.

AU - Guzmán, F.

AU - Alba, M. P.

AU - Cuéllar, A.

AU - Thomas, M. C.

AU - López, M. C.

AU - Rosas, F.

AU - Velasco, V.

AU - González, J. M.

AU - Patarroyo, M. E.

AU - Puerta, C. J.

N1 - Funding Information: The authors wish to thank Marcela Mercado from Universidad Javeriana de Bogotá, Colombia for the statistical analysis and to Adriana Rodriguez from Universidad Militar Nueva Granada for supporting ELISA techniques. M.C. Thomas and M.C. Lopez were supported by P06-CTS-02242 and RD06/0021/0014 grants from PAI and FIS, Spain. FIDIC was supported by COLCIENCIAS Contract No. RC-2007.

PY - 2007/8

Y1 - 2007/8

N2 - The K1 peptide is an HLA-A*0201-restricted cytotoxic epitope derived from the Trypanosoma cruzi KMP-11 protein, this being the etiological agent of Chagas' disease. This work describes the K1 peptide's secondary structure and its recognition by sera from chagasic patients. Circular dichroism and NMR spectroscopy analysis revealed that the K1 peptide adopts an α-helical conformation. Fifty-six percent of individuals had anti-K1 and 86% anti-KMP-11 antibodies by ELISA in the chronic Chagas' group and 28 and 68% in the indeterminate Chagas' group, respectively. By contrast, no reactivity was observed in sera from healthy individuals and tuberculosis patients. Antibody response subclass specificity to the K1 peptide was IgG1 and IgG3. Taken together these results support the idea that the K1 peptide acts as a B-cell-inducer epitope during Chagas' disease.

AB - The K1 peptide is an HLA-A*0201-restricted cytotoxic epitope derived from the Trypanosoma cruzi KMP-11 protein, this being the etiological agent of Chagas' disease. This work describes the K1 peptide's secondary structure and its recognition by sera from chagasic patients. Circular dichroism and NMR spectroscopy analysis revealed that the K1 peptide adopts an α-helical conformation. Fifty-six percent of individuals had anti-K1 and 86% anti-KMP-11 antibodies by ELISA in the chronic Chagas' group and 28 and 68% in the indeterminate Chagas' group, respectively. By contrast, no reactivity was observed in sera from healthy individuals and tuberculosis patients. Antibody response subclass specificity to the K1 peptide was IgG1 and IgG3. Taken together these results support the idea that the K1 peptide acts as a B-cell-inducer epitope during Chagas' disease.

KW - Chagas' disease

KW - Humoral immune response

KW - K1 peptide

KW - KMP-11 protein

KW - Trypanosoma cruzi

KW - α-Helix

UR - https://www.scopus.com/pages/publications/34547857754

U2 - 10.1016/j.peptides.2007.06.017

DO - 10.1016/j.peptides.2007.06.017

M3 - Article

C2 - 17683828

AN - SCOPUS:34547857754

SN - 0196-9781

VL - 28

SP - 1520

EP - 1526

JO - Peptides

JF - Peptides

IS - 8

ER -

Immunological and structural characterization of an epitope from the Trypanosoma cruzi KMP-11 protein

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