Immunochemical Recognition of Bothrops rhombeatus Venom by Two Polyvalent Antivenoms

Título traducido de la contribución: Reconocimiento inmunoquímico del veneno de Bothrops rhombeatus por dos antivenenos polivalentes

Karen Sarmiento, Jorge Zambrano, Carlos Galvis, Álvaro Molina-Olivares, Marisol Margarita Villadiego-Molinares, Johanna Alejandra Ramírez-Martínez, Ana Lucía Castiblanco, Fabio A. Aristizabal

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Resumen

The protein profile of Bothrops rhombeatus venom was compared to Bothrops asper and
Bothrops atrox, and the effectiveness of antivenoms from the National Institute of Health of Colombia (INS) and Antivipmyn-Tri (AVP-T) of Mexico were analyzed. Protein profiles were studied
with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and reverse-phase
high-performance liquid chromatography (RP-HPLC). The neutralizing potency and the level of
immunochemical recognition of the antivenoms to the venoms were determined using Western
blot, affinity chromatography, and enzyme-linked immunosorbent assay (ELISA). Bands of phospholipase A2 (PLA2), metalloproteinases (svMPs) I, II, and III as well as serine proteinases (SPs) in
the venom of B. rhombeatus were recognized by SDS-PAGE. With Western blot, both antivenoms
showed immunochemical recognition towards PLA2 and svMP. INS showed 94% binding to B.
rhombeatus venom and 92% to B. asper while AVP-T showed 90.4% binding to B. rhombeatus venom
and 96.6% to B. asper. Both antivenoms showed binding to PLA2 and svMP, with greater specificity
of AVP-T towards B. rhombeatus. Antivenom neutralizing capacity was calculated by species and
mL of antivenom, finding the following for INS: B. asper 6.6 mgV/mL, B. atrox 5.5 mgV/mL, and B.
rhombeatus 1.3 mgV/mL. Meanwhile, for AVP-T, the following neutralizing capacities were found:
B. asper 2.7 mgV/mL, B. atrox 2.1 mgV/mL, and B. rhombeatus 1.4 mgV/mL. These results show
that both antivenoms presented similarity between calculated neutralizing capacities in our trial,
reported in a product summary for the public for the B. asper species; however, this does not apply
to the other species tested in this trial.
Título traducido de la contribuciónReconocimiento inmunoquímico del veneno de Bothrops rhombeatus por dos antivenenos polivalentes
Idioma originalInglés
Páginas1-17
Número de páginas17
Volumen16
N.º3
Publicación especializadaToxins
EditorMultidisciplinary Digital Publishing Institute (MDPI)
DOI
EstadoPublicada - 14 mar. 2024

Palabras clave

  • Antivenins
  • Bothrops
  • Venom
  • Snake bites

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