TY - JOUR
T1 - Identification of malassezia species as part of normal skin and ear canal microbiota in horses
AU - Castañeda-Salazar, Rubiela
AU - Rodríguez-Sandoval, Diana M.
AU - Pulido-Villamarín, Adriana P.
AU - Linares-Linares, Melva Y.
AU - Oliver-Espinosa, Olimpo J.
N1 - Publisher Copyright:
© 2020, Universidad de Antioquia. All rights reserved.
PY - 2020/1
Y1 - 2020/1
N2 - Background: The yeasts of the genus Malassezia are considered part of the normal skin microbiota in humans and animals. In horses, several species of the genus Malassezia have been reported in different areas of the skin and ear canal. Objective: Isolate, characterize and identify the different species belonging to the genus Malassezia isolated from the ear canal and skin of equine patients with no dermatological lesions that were referred to the large animal clinic of veterinary teaching hospital at the National University of Colombia. Methods: 22 horses were evaluated and sampled. Eighty-two samples were obtained by swabbing either the ear canals (left and right), skin areas of prepuce, mammary gland and inguinal region. The samples were examined by cytological evaluation and were cultured on modified Dixon’s agar and phenotypic and molecular identification were performed for yeast colonies. Results: Fourteen yeast isolates were obtained from the 82 samples. Biochemical identification determined that 50% (n=7) were Malassezia spp., 35.7% (n=5) were identified as Candida spp. and 14.3% (n=2) as Cryptococcus spp.. Using molecular tests, the Malassezia species were M. slooffiae (28.6%) and M. nana (57.1%); only one isolate was classified as Trichosporo asahii. Conclusion: M. nana and M. slooffiae were identified as part of the normal ear canal and skin microbiota in the evaluated horses. The observed prevalence of Malassezia spp. was 18.2% (n=4/22) in this study sample.
AB - Background: The yeasts of the genus Malassezia are considered part of the normal skin microbiota in humans and animals. In horses, several species of the genus Malassezia have been reported in different areas of the skin and ear canal. Objective: Isolate, characterize and identify the different species belonging to the genus Malassezia isolated from the ear canal and skin of equine patients with no dermatological lesions that were referred to the large animal clinic of veterinary teaching hospital at the National University of Colombia. Methods: 22 horses were evaluated and sampled. Eighty-two samples were obtained by swabbing either the ear canals (left and right), skin areas of prepuce, mammary gland and inguinal region. The samples were examined by cytological evaluation and were cultured on modified Dixon’s agar and phenotypic and molecular identification were performed for yeast colonies. Results: Fourteen yeast isolates were obtained from the 82 samples. Biochemical identification determined that 50% (n=7) were Malassezia spp., 35.7% (n=5) were identified as Candida spp. and 14.3% (n=2) as Cryptococcus spp.. Using molecular tests, the Malassezia species were M. slooffiae (28.6%) and M. nana (57.1%); only one isolate was classified as Trichosporo asahii. Conclusion: M. nana and M. slooffiae were identified as part of the normal ear canal and skin microbiota in the evaluated horses. The observed prevalence of Malassezia spp. was 18.2% (n=4/22) in this study sample.
KW - Culture
KW - Ear canal
KW - Horses
KW - Identification
KW - Malassezia
KW - Normal microbiota
KW - PCR
KW - Skin
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=85079139617&partnerID=8YFLogxK
U2 - 10.17533/udea.rccp.v33n1a01
DO - 10.17533/udea.rccp.v33n1a01
M3 - Article
AN - SCOPUS:85079139617
SN - 0120-0690
VL - 33
SP - 5
EP - 15
JO - Revista Colombiana de Ciencias Pecuarias
JF - Revista Colombiana de Ciencias Pecuarias
IS - 1
ER -