Resumen
Objective: To evaluate probiotic properties of two native strains of S. cerevisiae. Materials and methods: Strains were used in assays for tolerance to biliary salts, pH, temperature and adhesion to Salmonella spp., E. coli and Shigella spp. and antagonism. A33 × 3 factorial design was used, with three levels for each factor (strains, pH and initial concentration of substrate (S0)) by triplicate, to establish the culture conditions for each strain. A commercial strain (B) was used as control. The selected strain was cultured in a 2L bioreactor; the biomass was dryed by temperature and the resulting product was assayed to determine N2 concentration and cellular viability. Results. Strain A (from sugar cane), tolerated pH 3 ± 0.2, 0.3% (w/v) of biliary salts and 42°C. The factorial ANOVA found significant differences between the 27 assays (p<0.05) and surface analysis found that the interaction between factors strain and S 0 was significant, suggesting for the optimization process the use of strain A and increasing concentrations of S0. Results: were reproduced in bioreactor with mx 0.31h-1, dt 2.18h and Y(x/s) 0.126g/g. the dry biomass obtained was viable and yielded between 6.3 and 6.9% N2/g. Conclusions: Native strains were identified with probiotic properties such as pH, biliary salt and temperature tolerance, and adherence to Salmonella spp., E.coli and Shigella spp.
Título traducido de la contribución | In vitro preliminary identification of probiotic properties of S. cerevisiae strains |
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Idioma original | Español |
Páginas (desde-hasta) | 1157-1169 |
Número de páginas | 13 |
Publicación | Revista MVZ Cordoba |
Volumen | 13 |
N.º | 1 |
Estado | Publicada - 2008 |
Publicado de forma externa | Sí |
Palabras clave
- Answer surface
- Probiotic
- Saccharomyces cerevisiae