TY - JOUR
T1 - Genotyping of Malassezia pachydermatis disclosed genetic variation in isolates from dogs in Colombia1
AU - Celis-Ramírez, Adriana Marcela
AU - Guevara-Suarez, Marcela
AU - Galvis-Marín, Juan Camilo
AU - Rodríguez-Bocanegra, María Ximena
AU - Castañeda-Salazar, Rubiela
AU - Linares-Linares, Melva Yomary
AU - Triana-Sierra, Sergio Heli
AU - del Pilar Pulido-Villamarín, Adriana
N1 - Publisher Copyright:
© 2019 Colegio Brasileiro de Patologia Animal. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Malassezia pachydermatis is a lipophilic and lipid-dependent yeast mostly isolated from animals’ skin; hence, it is regarded as a zoophilic species causing otitis externa in dogs. Aspects associated with its epidemiology and pathogenicity is a matter of interest. This study aimed to conduct a molecular characterization of 43 isolates of M. pachydermatis obtained from dogs with otitis externa. For this purpose, the 5.8S internal transcribed spacer 2 (ITS2) and D1/D2 26S rRNA regions were amplified, sequenced and analyzed using restriction fragment length polymorphism (RFLP) with AluI, CfoI, and BstF5I endonucleases. Phylogenetic analyses revealed that these isolates grouped with the sequence types I, IV and V, previously proposed for M. pachydermatis. Interestingly, we found a new polymorphic RFLP pattern using BstF5I, these isolates were associated with the sequence types IV and V, nevertheless an association between polymorphic RFLP patterns, and fosfolipase activity or canine population data was not observed. These findings underline the genetic diversity of M. pachydermatis and provide new insights about the epidemiology of this species in the analyzed population.
AB - Malassezia pachydermatis is a lipophilic and lipid-dependent yeast mostly isolated from animals’ skin; hence, it is regarded as a zoophilic species causing otitis externa in dogs. Aspects associated with its epidemiology and pathogenicity is a matter of interest. This study aimed to conduct a molecular characterization of 43 isolates of M. pachydermatis obtained from dogs with otitis externa. For this purpose, the 5.8S internal transcribed spacer 2 (ITS2) and D1/D2 26S rRNA regions were amplified, sequenced and analyzed using restriction fragment length polymorphism (RFLP) with AluI, CfoI, and BstF5I endonucleases. Phylogenetic analyses revealed that these isolates grouped with the sequence types I, IV and V, previously proposed for M. pachydermatis. Interestingly, we found a new polymorphic RFLP pattern using BstF5I, these isolates were associated with the sequence types IV and V, nevertheless an association between polymorphic RFLP patterns, and fosfolipase activity or canine population data was not observed. These findings underline the genetic diversity of M. pachydermatis and provide new insights about the epidemiology of this species in the analyzed population.
UR - http://www.scopus.com/inward/record.url?scp=85082295345&partnerID=8YFLogxK
U2 - 10.1590/1678-5150-PVB-6158
DO - 10.1590/1678-5150-PVB-6158
M3 - Article
AN - SCOPUS:85082295345
SN - 0100-736X
VL - 39
SP - 915
EP - 922
JO - Pesquisa Veterinaria Brasileira
JF - Pesquisa Veterinaria Brasileira
IS - 11
ER -