TY - JOUR
T1 - Generation of organotypic multicellular spheres by magnetic levitation
T2 - Model for the study of human hematopoietic stem cells microenvironment
AU - Mejía-Cruz, Claudia Camila
AU - Barreto-Durán, Emilia
AU - Pardo-Pérez, María Alejandra
AU - Jimenez, María Camila
AU - Rincón, Julieth
AU - Vanegas, Karen
AU - Rodríguez, Jorge Luis
AU - Jaramillo-Garcia, Luis Fernando
AU - Ulloa, Juan Carlos
AU - Díaz, Rodolfo Martínez
AU - Leal-García, Efrain
AU - Pérez-Núñez, Rafael
AU - Barreto, Alfonso
AU - Rodríguez-Pardo, Viviana M.
N1 - Publisher Copyright:
© 2019 by the Korean Society for Stem Cell Research.
PY - 2019
Y1 - 2019
N2 - Background and Objective: The characteristics of human hematopoietic stem cells are conditioned by the microenvironment of the bone marrow, where they interact with other cell populations, such as mesenchymal stem cells and endothelial cells; however, the study of this microenvironment is complex. The objective of this work was to develop a 3D culture system by magnetic levitation that imitates the microenvironment of human HSC. Methods and Results: Human bone marrow-mesenchymal stem cells, umbilical cord blood-hematopoietic stem cells and a non-tumoral endothelial cell line (CC2811, Lonza®) were used to develop organotypic multicellular spheres by the magnetic levitation method. We obtained viable structures with an average sphericity index greater than 0.6, an average volume of 0.5 mm3 and a percentage of aggregation greater than 70%. Histological studies of the organotypic multicellular spheres used hematoxylin and eosin stains, and an evaluation of vimentin expression by means of immunohistochemistry demonstrated an organized internal structure without picnotic cells and a high expression of vimentin. The functional capacity of human hematopoietic stem cells after organotypic multicellular spheres culture was evaluated by multipotency tests, and it was demonstrated that 3D structures without exogenous Flt3L are autonomous in the maintenance of multipotency of human hematopoietic stem cells. Conclusions: We developed organotypic multicellular spheres from normal human cells that mimic the microenvironment of the human hematopoietic stem cells. These structures are the prototype for the development of complex organoids that allow the further study of the biology of normal human stem cells and their potential in regenerative medicine.
AB - Background and Objective: The characteristics of human hematopoietic stem cells are conditioned by the microenvironment of the bone marrow, where they interact with other cell populations, such as mesenchymal stem cells and endothelial cells; however, the study of this microenvironment is complex. The objective of this work was to develop a 3D culture system by magnetic levitation that imitates the microenvironment of human HSC. Methods and Results: Human bone marrow-mesenchymal stem cells, umbilical cord blood-hematopoietic stem cells and a non-tumoral endothelial cell line (CC2811, Lonza®) were used to develop organotypic multicellular spheres by the magnetic levitation method. We obtained viable structures with an average sphericity index greater than 0.6, an average volume of 0.5 mm3 and a percentage of aggregation greater than 70%. Histological studies of the organotypic multicellular spheres used hematoxylin and eosin stains, and an evaluation of vimentin expression by means of immunohistochemistry demonstrated an organized internal structure without picnotic cells and a high expression of vimentin. The functional capacity of human hematopoietic stem cells after organotypic multicellular spheres culture was evaluated by multipotency tests, and it was demonstrated that 3D structures without exogenous Flt3L are autonomous in the maintenance of multipotency of human hematopoietic stem cells. Conclusions: We developed organotypic multicellular spheres from normal human cells that mimic the microenvironment of the human hematopoietic stem cells. These structures are the prototype for the development of complex organoids that allow the further study of the biology of normal human stem cells and their potential in regenerative medicine.
KW - Hematopoietic stem cells
KW - Mesenchymal stem cells
KW - Microenvironment
KW - Organoids
KW - Organotypic multicellular spheres
UR - http://www.scopus.com/inward/record.url?scp=85065519168&partnerID=8YFLogxK
U2 - 10.15283/ijsc18061
DO - 10.15283/ijsc18061
M3 - Article
AN - SCOPUS:85065519168
SN - 2005-3606
VL - 12
SP - 51
EP - 62
JO - International Journal of Stem Cells
JF - International Journal of Stem Cells
IS - 1
ER -