Follow‑up study of HPV58 variants in women with incident HPV58 infection from a Colombian cohort

Mónica Molano; Oscar Buitrago; Pablo Moreno-Acosta; Suzanne M. Garland; Nicolás Morales; Antonio Huertas; Teresa Martinez; Oscar Gamboa; Sepehr N. Tabrizi; Alyssa Cornall; Nubia Muñoz

doi:10.3892/ol.2018.8928

Follow‑up study of HPV58 variants in women with incident HPV58 infection from a Colombian cohort

Título traducido de la contribución: Estudio de seguimiento de variantes del virus de papiloma humano (HPV58) en mujeres con infección incidente por HPV58 de una cohorte colombiana.

Mónica Molano, Oscar Buitrago, Pablo Moreno-Acosta, Suzanne M. Garland, Nicolás Morales, Antonio Huertas, Teresa Martinez, Oscar Gamboa, Sepehr N. Tabrizi, Alyssa Cornall, Nubia Muñoz

Producción: Contribución a una revista › Artículo › revisión exhaustiva

5 Citas (Scopus)

Resumen

Ciertas variantes del virus del papiloma humano (VPH) tipo 58 se asocian con un mayor riesgo de lesiones intraepiteliales escamosas de alto grado y cáncer de cuello uterino. Sin embargo, se sabe poco sobre la persistencia de las variantes E6/E7 del VPH58 en mujeres con infecciones incidentes por VPH58. El objetivo del presente estudio fue evaluar la presencia y persistencia de las variantes E6/E7 del VPH58 en 71 mujeres con infección incidente por VPH58 a lo largo de su seguimiento. Estas mujeres pertenecían a una cohorte examinada en un estudio longitudinal de 1.610 mujeres colombianas, que eran VPH-negativas y tenían citología basal normal. Las regiones de ADN E6/E7 de muestras HPV58-positivas se amplificaron y secuenciaron mediante secuenciación directa automatizada. Se analizaron un total de 639 muestras de las 71 mujeres, y 117 muestras (18,3%) fueron HPV58-positivas. Se detectaron las variantes E6/E7 del VPH58 en el 85,5% de las muestras. La variante T307/A694/G744/A761 se identificó en el 88% de las muestras, la variante T307/G744 se identificó en el 9% de las muestras y la variante T187/T307/A367/G744/G793/T798/A801/T840/C852 se identificó en el 3% de las muestras. En general, el 50% de las infecciones por VPH58 estaban presentes después de 1 año de seguimiento y todas las infecciones se eliminaron después de 7 años. Las mujeres que tuvieron su primera relación sexual a los >15 años de edad tuvieron una tasa de eliminación menor que las que tuvieron su primera relación sexual a los ≤15 años de edad [cociente de riesgos instantáneos (HR) = 0,29; intervalo de confianza (IC) del 95% = 0,09-0,92]. Asimismo, las mujeres que habían tenido hijos presentaron una mayor tasa de eliminación que las nulíparas (HR = 3,43; IC del 95 % = 1,23-9,60). No se observaron diferencias en las tasas de eliminación entre las variantes E6/E7 del VPH58. En conclusión, las variantes del VPH58 no se asociaron con la persistencia de la infección en este grupo de mujeres.

Título traducido de la contribución	Estudio de seguimiento de variantes del virus de papiloma humano (HPV58) en mujeres con infección incidente por HPV58 de una cohorte colombiana.
Idioma original	Inglés
Páginas (desde-hasta)	2511-2516
Número de páginas	6
Publicación	Oncology Letters
Volumen	16
N.º	2
DOI	https://doi.org/10.3892/ol.2018.8928
Estado	Publicada - ago. 2018
Publicado de forma externa	Sí

Palabras clave

Estudios de seguimiento
Variación genómica
Virus del papiloma humano
Infección persistente

ODS de las Naciones Unidas

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10.3892/ol.2018.8928

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@article{88f9a6b1441b4d5fb8bf8f18547edcf3,

title = "Follow‑up study of HPV58 variants in women with incident HPV58 infection from a Colombian cohort",

abstract = "Certain variants of human papillomavirus (HPV) type 58 are associated with an increased risk of high grade squamous intraepithelial lesions and cervical cancer. However, little is known about the persistence of HPV58 E6/E7 variants in women with incident HPV58 infections. The aim of the present study was to evaluate the presence and persistence of HPV58 E6/E7 variants in 71 women with incident HPV58 infection throughout their follow-up. These women belonged to a cohort examined in a longitudinal study of 1,610 Colombian women, who were HPV-negative and had normal baseline cytology. E6/E7 DNA regions of HPV58-positive samples were amplified and sequenced using automated direct sequencing. A total of 639 samples were analyzed from the 71 women, and 117 samples (18.3\%) were HPV58-positive. HPV58 E6/E7 variants were detected in 85.5\% of the samples. The T307/A694/G744/A761 variant was identified in 88\% of the samples, the T307/G744 variant was identified in 9\% of samples and the T187/T307/A367/G744/G793/T798/A801/T840/C852 was identified in 3\% of the samples. Overall, 50\% of the HPV58 infections were present after 1 year of follow-up and all infections were cleared after 7 years. Women who had first sexual intercourse at >15 years of age had a lower clearance rate than those who had sexual intercourse for the first time at ≤15 years of age [hazard ratio (HR)=0.29; 95\% confidence interval (CI)=0.09-0.92]. Likewise, parous women had a higher clearance rate than nulliparous women (HR=3.43, 95\% CI=1.23-9.60). There was no difference in clearance rates between HPV58 E6/E7 variants. In conclusion, HPV58 variants were not associated with persistence of the infection in this group of women.",

keywords = "Follow-up studies, Genomic variation, Human papilloma virus, Persistent infection, Estudios de seguimiento, Variaci{\'o}n gen{\'o}mica, Virus del papiloma humano, Infecci{\'o}n persistente",

author = "M{\'o}nica Molano and Oscar Buitrago and Pablo Moreno-Acosta and Garland, \{Suzanne M.\} and Nicol{\'a}s Morales and Antonio Huertas and Teresa Martinez and Oscar Gamboa and Tabrizi, \{Sepehr N.\} and Alyssa Cornall and Nubia Mu{\~n}oz",

year = "2018",

month = aug,

doi = "10.3892/ol.2018.8928",

language = "English",

volume = "16",

pages = "2511--2516",

journal = "Oncology Letters",

issn = "1792-1074",

publisher = "Spandidos Publications",

number = "2",

}

TY - JOUR

T1 - Follow‑up study of HPV58 variants in women with incident HPV58 infection from a Colombian cohort

AU - Molano, Mónica

AU - Buitrago, Oscar

AU - Moreno-Acosta, Pablo

AU - Garland, Suzanne M.

AU - Morales, Nicolás

AU - Huertas, Antonio

AU - Martinez, Teresa

AU - Gamboa, Oscar

AU - Tabrizi, Sepehr N.

AU - Cornall, Alyssa

AU - Muñoz, Nubia

PY - 2018/8

Y1 - 2018/8

N2 - Certain variants of human papillomavirus (HPV) type 58 are associated with an increased risk of high grade squamous intraepithelial lesions and cervical cancer. However, little is known about the persistence of HPV58 E6/E7 variants in women with incident HPV58 infections. The aim of the present study was to evaluate the presence and persistence of HPV58 E6/E7 variants in 71 women with incident HPV58 infection throughout their follow-up. These women belonged to a cohort examined in a longitudinal study of 1,610 Colombian women, who were HPV-negative and had normal baseline cytology. E6/E7 DNA regions of HPV58-positive samples were amplified and sequenced using automated direct sequencing. A total of 639 samples were analyzed from the 71 women, and 117 samples (18.3%) were HPV58-positive. HPV58 E6/E7 variants were detected in 85.5% of the samples. The T307/A694/G744/A761 variant was identified in 88% of the samples, the T307/G744 variant was identified in 9% of samples and the T187/T307/A367/G744/G793/T798/A801/T840/C852 was identified in 3% of the samples. Overall, 50% of the HPV58 infections were present after 1 year of follow-up and all infections were cleared after 7 years. Women who had first sexual intercourse at >15 years of age had a lower clearance rate than those who had sexual intercourse for the first time at ≤15 years of age [hazard ratio (HR)=0.29; 95% confidence interval (CI)=0.09-0.92]. Likewise, parous women had a higher clearance rate than nulliparous women (HR=3.43, 95% CI=1.23-9.60). There was no difference in clearance rates between HPV58 E6/E7 variants. In conclusion, HPV58 variants were not associated with persistence of the infection in this group of women.

AB - Certain variants of human papillomavirus (HPV) type 58 are associated with an increased risk of high grade squamous intraepithelial lesions and cervical cancer. However, little is known about the persistence of HPV58 E6/E7 variants in women with incident HPV58 infections. The aim of the present study was to evaluate the presence and persistence of HPV58 E6/E7 variants in 71 women with incident HPV58 infection throughout their follow-up. These women belonged to a cohort examined in a longitudinal study of 1,610 Colombian women, who were HPV-negative and had normal baseline cytology. E6/E7 DNA regions of HPV58-positive samples were amplified and sequenced using automated direct sequencing. A total of 639 samples were analyzed from the 71 women, and 117 samples (18.3%) were HPV58-positive. HPV58 E6/E7 variants were detected in 85.5% of the samples. The T307/A694/G744/A761 variant was identified in 88% of the samples, the T307/G744 variant was identified in 9% of samples and the T187/T307/A367/G744/G793/T798/A801/T840/C852 was identified in 3% of the samples. Overall, 50% of the HPV58 infections were present after 1 year of follow-up and all infections were cleared after 7 years. Women who had first sexual intercourse at >15 years of age had a lower clearance rate than those who had sexual intercourse for the first time at ≤15 years of age [hazard ratio (HR)=0.29; 95% confidence interval (CI)=0.09-0.92]. Likewise, parous women had a higher clearance rate than nulliparous women (HR=3.43, 95% CI=1.23-9.60). There was no difference in clearance rates between HPV58 E6/E7 variants. In conclusion, HPV58 variants were not associated with persistence of the infection in this group of women.

KW - Follow-up studies

KW - Genomic variation

KW - Human papilloma virus

KW - Persistent infection

KW - Estudios de seguimiento

KW - Variación genómica

KW - Virus del papiloma humano

KW - Infección persistente

UR - https://www.scopus.com/pages/publications/85049451395

U2 - 10.3892/ol.2018.8928

DO - 10.3892/ol.2018.8928

M3 - Article

AN - SCOPUS:85049451395

SN - 1792-1074

VL - 16

SP - 2511

EP - 2516

JO - Oncology Letters

JF - Oncology Letters

IS - 2

ER -

Follow‑up study of HPV58 variants in women with incident HPV58 infection from a Colombian cohort

Resumen

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ODS de las Naciones Unidas

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