Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

F. Gamboa; J. M. Manterola; B. Vinado; L. Matas; M. Gimenez; J. Lonca; J. R. Manzano; C. Rodrigo; P. J. Cardona; E. Padilla; J. Dominguez; V. Ausina

doi:10.1128/jcm.35.1.307-310.1997

Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

F. Gamboa, J. M. Manterola, B. Vinado, L. Matas, M. Gimenez, J. Lonca, J. R. Manzano, C. Rodrigo, P. J. Cardona, E. Padilla, J. Dominguez, V. Ausina

Generalitat de Catalunya

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37 Citas (Scopus)

Resumen

The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 μl of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.

Idioma original	Inglés
Páginas (desde-hasta)	307-310
Número de páginas	4
Publicación	Journal of Clinical Microbiology
Volumen	35
N.º	1
DOI	https://doi.org/10.1128/jcm.35.1.307-310.1997
Estado	Publicada - 1997
Publicado de forma externa	Sí

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Gamboa, F., Manterola, J. M., Vinado, B., Matas, L., Gimenez, M., Lonca, J., Manzano, J. R., Rodrigo, C., Cardona, P. J., Padilla, E., Dominguez, J., & Ausina, V. (1997). Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test. Journal of Clinical Microbiology, 35(1), 307-310. https://doi.org/10.1128/jcm.35.1.307-310.1997

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title = "Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test",

abstract = "The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 μl of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.",

author = "F. Gamboa and Manterola, {J. M.} and B. Vinado and L. Matas and M. Gimenez and J. Lonca and Manzano, {J. R.} and C. Rodrigo and Cardona, {P. J.} and E. Padilla and J. Dominguez and V. Ausina",

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doi = "10.1128/jcm.35.1.307-310.1997",

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Gamboa, F, Manterola, JM, Vinado, B, Matas, L, Gimenez, M, Lonca, J, Manzano, JR, Rodrigo, C, Cardona, PJ, Padilla, E, Dominguez, J & Ausina, V 1997, 'Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test', Journal of Clinical Microbiology, vol. 35, n.º 1, pp. 307-310. https://doi.org/10.1128/jcm.35.1.307-310.1997

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T1 - Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

AU - Gamboa, F.

AU - Manterola, J. M.

AU - Vinado, B.

AU - Matas, L.

AU - Gimenez, M.

AU - Lonca, J.

AU - Manzano, J. R.

AU - Rodrigo, C.

AU - Cardona, P. J.

AU - Padilla, E.

AU - Dominguez, J.

AU - Ausina, V.

PY - 1997

Y1 - 1997

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AB - The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 μl of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.

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SN - 0095-1137

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

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Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe amplified Mycobacterium tuberculosis direct test

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