Differential Expression of IgM and IgD Discriminates Two Subpopulations of Human Circulating IgM⁺IgD⁺CD27⁺ B Cells That Differ Phenotypically, Functionally, and Genetically

The origin and function of blood IgM⁺IgD⁺CD27⁺ B cells is controversial, and they are considered a heterogeneous population. Previous staining of circulating B cells of healthy donors with rotavirus fluorescent virus-like particles allowed us to differentiate two subsets of IgM⁺IgD⁺CD27⁺: IgM^hi and IgM^lo B cells. Here, we confirmed this finding and compared the phenotype, transcriptome, in vitro function, and Ig gene repertoire of these two subsets. Eleven markers phenotypically discriminated both subsets (CD1c, CD69, IL21R, CD27, MTG, CD45RB, CD5, CD184, CD23, BAFFR, and CD38) with the IgM^hi phenotypically resembling previously reported marginal zone B cells and the IgM^lo resembling both naïve and memory B cells. Transcriptomic analysis showed that both subpopulations clustered close to germinal center-experienced IgM only B cells with a Principal Component Analysis, but differed in expression of 78 genes. Moreover, IgM^hi B cells expressed genes characteristic of previously reported marginal zone B cells. After stimulation with CpG and cytokines, significantly (p < 0.05) higher frequencies (62.5%) of IgM^hi B cells proliferated, compared with IgM^lo B cells (35.37%), and differentiated to antibody secreting cells (14.22% for IgM^hi and 7.19% for IgM^lo). IgM^hi B cells had significantly (p < 0.0007) higher frequencies of mutations in IGHV and IGKV regions, IgM^lo B cells had higher usage of IGHJ6 genes (p < 0.0001), and both subsets differed in their HCDR3 properties. IgM^hi B cells shared most of their shared IGH clonotypes with IgM only memory B cells, and IgM^lo B cells with IgM^hi B cells. These results support the notion that differential expression of IgM and IgD discriminates two subpopulations of human circulating IgM⁺IgD⁺CD27⁺ B cells, with the IgM^hi B cells having similarities with previously described marginal zone B cells that passed through germinal centers, and the IgM^lo B cells being the least differentiated amongst the IgM⁺CD27⁺ subsets.