TY - JOUR
T1 - Cellular target of isoquercetin from Passiflora ligularis Juss for glucose uptake in rat soleus muscle
AU - Rey, Diana
AU - Fernandes, Thaís Alves
AU - Sulis, Paola Miranda
AU - Gonçalves, Renata
AU - Sepúlveda R, Michelle
AU - Silva Frederico, Marisa Jádna
AU - Aragon, Marcela
AU - Ospina, Luís Fernando
AU - Costa, Geison M.
AU - Silva, Fátima Regina Mena Barreto
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Quercetin 3-O-beta-D-glucopyranoside (isoquercetin) is one of the most frequent metabolites of the Passiflora ligularis Juss. The purpose of this study was to investigate the effect of the aqueous extract and ethanol fraction from P. ligularis Juss leaves on glycaemia and the mechanism of action of isoquercetin on glucose uptake. In the glucose tolerance test, the aqueous extract and ethanol fraction from P. ligularis Juss (125 mg/kg to 500 mg/kg o. g.) reduced glycaemia and increased the hepatic and muscular glycogen content. Phytochemical analysis evidenced the dominant presence of isoquercetin in the extract and fraction from leaves of P. ligularis Juss. Isoquercetin mediates the stimulatory effect on glucose uptake independent of insulin receptor activation but, involve PI3K, MAPK, MEK/ERK pathways and de novo protein synthesis to GLUT-4 translocation. Overall findings revealed that isoquercetin and aqueous extract and ethanol fraction of P. ligularis Juss leaves might be a promising functional food or medicine for the treatment or prevention of diabetes.
AB - Quercetin 3-O-beta-D-glucopyranoside (isoquercetin) is one of the most frequent metabolites of the Passiflora ligularis Juss. The purpose of this study was to investigate the effect of the aqueous extract and ethanol fraction from P. ligularis Juss leaves on glycaemia and the mechanism of action of isoquercetin on glucose uptake. In the glucose tolerance test, the aqueous extract and ethanol fraction from P. ligularis Juss (125 mg/kg to 500 mg/kg o. g.) reduced glycaemia and increased the hepatic and muscular glycogen content. Phytochemical analysis evidenced the dominant presence of isoquercetin in the extract and fraction from leaves of P. ligularis Juss. Isoquercetin mediates the stimulatory effect on glucose uptake independent of insulin receptor activation but, involve PI3K, MAPK, MEK/ERK pathways and de novo protein synthesis to GLUT-4 translocation. Overall findings revealed that isoquercetin and aqueous extract and ethanol fraction of P. ligularis Juss leaves might be a promising functional food or medicine for the treatment or prevention of diabetes.
KW - Diabetes
KW - Flavonoid
KW - GLUT-4
KW - Isoquercetin
KW - Passiflora ligularis
UR - http://www.scopus.com/inward/record.url?scp=85089410543&partnerID=8YFLogxK
U2 - 10.1016/j.cbi.2020.109198
DO - 10.1016/j.cbi.2020.109198
M3 - Article
C2 - 32692981
AN - SCOPUS:85089410543
SN - 0009-2797
VL - 330
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
M1 - 109198
ER -
