TY - JOUR
T1 - Carbon source feeding strategies for recombinant protein expression in Pichia pastoris and Pichia methanolica
AU - Poutou-Piñales, Raúl A.
AU - Córdoba-Ruiz, Henry A.
AU - Barrera-Avellaneda, Luis A.
AU - Delgado-Boada, Julio M.
PY - 2010/4/12
Y1 - 2010/4/12
N2 - Pichia pastoris and Pichia methanolica have been used as expression systems for the production of recombinant protein. The main problems of the production are the slow hierarchic consumption of ethanol and acetate which cause toxicity problems due to methanol accumulation when this surpasses 0.5 gl-1.In some cases, the laboratory scale cultures does not show the methanol accumulation problems because the cells are usually washed before changing the depleted carbon source culture media, by a fresh one, supplemented with methanol; a strategy that is clearly inapplicable in the industrial productions. Other authors use to feed the methanol before the depletion of glycerol or D-glucose, but this practice does not guaranty the ethanol and acetate fast consumption; leading to methanol accumulation and toxicity problems. It was concluded that pre-induction stage strategy should be studied in detail and that it is very important to start the induction stage with a concentration of biomass as great as possible. On the other side, it is essential that there should be a monitoring of ethanol and acetate until reaching non-toxic methanol stable-concentration and these conditions should be maintained till the end of the process.
AB - Pichia pastoris and Pichia methanolica have been used as expression systems for the production of recombinant protein. The main problems of the production are the slow hierarchic consumption of ethanol and acetate which cause toxicity problems due to methanol accumulation when this surpasses 0.5 gl-1.In some cases, the laboratory scale cultures does not show the methanol accumulation problems because the cells are usually washed before changing the depleted carbon source culture media, by a fresh one, supplemented with methanol; a strategy that is clearly inapplicable in the industrial productions. Other authors use to feed the methanol before the depletion of glycerol or D-glucose, but this practice does not guaranty the ethanol and acetate fast consumption; leading to methanol accumulation and toxicity problems. It was concluded that pre-induction stage strategy should be studied in detail and that it is very important to start the induction stage with a concentration of biomass as great as possible. On the other side, it is essential that there should be a monitoring of ethanol and acetate until reaching non-toxic methanol stable-concentration and these conditions should be maintained till the end of the process.
KW - Carbon source
KW - Pichia methanolica
KW - Pichia pastoris
KW - Protein expression
UR - http://www.scopus.com/inward/record.url?scp=77951246140&partnerID=8YFLogxK
M3 - Review article
AN - SCOPUS:77951246140
SN - 1684-5315
VL - 9
SP - 2173
EP - 2184
JO - African Journal of Biotechnology
JF - African Journal of Biotechnology
IS - 15
ER -