TY - JOUR
T1 - Biomarkers for the severity of periodontal disease in patients with obstructive sleep apnea:IL-1 β, IL-6, IL-17A, and IL-33
AU - Corral, Mayra A. Téllez
AU - EDDY, HERRERA
AU - Jimenez, Natalia Arango
AU - Vera, Darena Z. Morales
AU - Velosa-Porras, Juliana
AU - Uriza, Catalina Latorre
AU - Arregoces, Francina M. Escobar
AU - Martinez, Patricia Hidalgo
AU - Cortés, María E.
AU - Otero, Liliana
AU - Giraldo, Claudia M. Parra
AU - Nelly, S Roa M
N1 - Publisher Copyright:
© 2023 The Author(s)
© 2023 The Author(s).
PY - 2023/3
Y1 - 2023/3
N2 - Objective: This study aims to compare the salivary and gingival crevicular fluid (GCF) concentrations of five cytokines: IL-1β, IL-6, IL-17A, IL-33, and Tumor Necrosis Factor-alpha (TNF-α) in patients with OSA and their association with periodontitis. Methods: Samples of saliva and GCF were obtained from 84 patients classified into four groups according to periodontal and OSA diagnosis: G1(H) healthy patients, G2(P) periodontitis and non-OSA patients, G3(OSA) OSA and non-periodontitis patients, and G4(P-OSA) periodontitis and OSA patients. The cytokines in the samples were quantified using multiplexed bead immunoassays. Data were analyzed with the Kruskal-Wallis test, Dunn's multiple comparisons test, and the Spearman correlation test. Results: Stage III periodontitis was the highest in patients with severe OSA (69%; p=0.0142). Similar levels of IL-1β and IL-6 in saliva were noted in G2(P) and G4(P-OSA). The IL-6, IL-17A and IL-33 levels were higher in the GCF of G4(P-OSA). There was a significant positive correlation between IL-33 in saliva and stage IV periodontitis in G4(P-OSA) (rs = 0.531). The cytokine profile of the patients in G4(P-OSA) with Candida spp. had an increase of the cytokine's levels compared to patients who did not have the yeast. Conclusions: OSA may increase the risk of developing periodontitis due to increase of IL-1β and IL-6 in saliva and IL-6, IL-17A and IL-33 in GCF that share the activation of the osteoclastogenesis. Those cytokines may be considered as biomarkers of OSA and periodontitis.
AB - Objective: This study aims to compare the salivary and gingival crevicular fluid (GCF) concentrations of five cytokines: IL-1β, IL-6, IL-17A, IL-33, and Tumor Necrosis Factor-alpha (TNF-α) in patients with OSA and their association with periodontitis. Methods: Samples of saliva and GCF were obtained from 84 patients classified into four groups according to periodontal and OSA diagnosis: G1(H) healthy patients, G2(P) periodontitis and non-OSA patients, G3(OSA) OSA and non-periodontitis patients, and G4(P-OSA) periodontitis and OSA patients. The cytokines in the samples were quantified using multiplexed bead immunoassays. Data were analyzed with the Kruskal-Wallis test, Dunn's multiple comparisons test, and the Spearman correlation test. Results: Stage III periodontitis was the highest in patients with severe OSA (69%; p=0.0142). Similar levels of IL-1β and IL-6 in saliva were noted in G2(P) and G4(P-OSA). The IL-6, IL-17A and IL-33 levels were higher in the GCF of G4(P-OSA). There was a significant positive correlation between IL-33 in saliva and stage IV periodontitis in G4(P-OSA) (rs = 0.531). The cytokine profile of the patients in G4(P-OSA) with Candida spp. had an increase of the cytokine's levels compared to patients who did not have the yeast. Conclusions: OSA may increase the risk of developing periodontitis due to increase of IL-1β and IL-6 in saliva and IL-6, IL-17A and IL-33 in GCF that share the activation of the osteoclastogenesis. Those cytokines may be considered as biomarkers of OSA and periodontitis.
KW - Cytokines
KW - Gingival crevicular fluid
KW - Obstructive sleep apnea
KW - Periodontitis
KW - Saliva
KW - Cytokines
KW - Gingival crevicular fluid
KW - Obstructive sleep apnea
KW - Periodontitis
KW - Saliva
UR - http://www.scopus.com/inward/record.url?scp=85150058169&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/27448cc0-ca19-31fa-bbe2-19861558b411/
U2 - 10.1016/j.heliyon.2023.e14340
DO - 10.1016/j.heliyon.2023.e14340
M3 - Article
C2 - 36967976
AN - SCOPUS:85150058169
SN - 2405-8440
VL - 9
JO - Heliyon
JF - Heliyon
IS - 3
M1 - e14340
ER -