TY - JOUR
T1 - Association between the proliferative rate of neoplastic B cells, their maturation stage, and underlying cytogenetic abnormalities in B-cell chronic lymphoproliferative disorders
T2 - Analysis of a series of 432 patients
AU - Quijano, Sandra
AU - López, Antonio
AU - Rasillo, Ana
AU - Barrena, Susana
AU - Sánchez, Maria Luz
AU - Flores, Juan
AU - Fernández, Carlos
AU - Sayagués, José Maria
AU - Osuna, Carlos Salvador
AU - Fernández, Nuria
AU - González, Marcos
AU - Giraldo, Pilar
AU - Giralt, Manuel
AU - Pérez, Maria Carmen
AU - Martin-Antoran, José Manuel
AU - Gutiérrez, Oliver
AU - Perdiguer, Luis
AU - Mediavilla, Joaquín Díaz
AU - Silva, Manuel González
AU - Rio, Agustín Asensio Del
AU - Cerveró, Carlos
AU - Guerra, José Luis
AU - Butrón, Rosario
AU - García, Maria Del Carmen
AU - Almeida, Julia
AU - Orfao, Alberto
PY - 2008/5/15
Y1 - 2008/5/15
N2 - Limited knowledge exists about the impact of specific genetic abnormalities on the proliferation of neoplastic B cells from chronic lymphoproliferative disorders (B- CLPDs). Here we analyze the impact of cytogenetic abnormalities on the proliferation of neoplastic B cells in 432 B-CLPD patients, grouped according to diagnosis and site of sampling, versus their normal counterparts. Overall, proliferation of neoplastic B cells highly varied among the different B-CLPD subtypes, the greatest numbers of proliferating cells being identified in diffuse large B-cell lymphoma (DLBCL) and Burkitt lymphoma (BL). Compared with normal B cells, neoplastic B-CLPD cells showed significantly increased S + G2/M-phase values in mantle cell lymphoma (MCL), B-chronic lymphocytic leukemia (B-CLL), BL, and some DLBCL cases. Conversely, decreased proliferation was observed in follicular lymphoma, lymphoplasmacytic lymphoma/ Waldenstrom macroglobulinemia (LPL/ WM), and some DLBCL patients; hairy cell leukemia, splenic marginal zone, and MALT-lymphoma patients showed S + G 2/ M phase values similar to normal mature B lymphocytes from LN. Interestingly, in B-CLL and MCL significantly higher percentages of S + G 2/M cells were detected in BM versus PB and in LN versus BM and PB samples, respectively. In turn, presence of 14q32.3 gene rearrangements and DNA aneuploidy, was associated with a higher percentage of S + G2/M-phase cells among LPL/WM and B-CLL cases, respectively.
AB - Limited knowledge exists about the impact of specific genetic abnormalities on the proliferation of neoplastic B cells from chronic lymphoproliferative disorders (B- CLPDs). Here we analyze the impact of cytogenetic abnormalities on the proliferation of neoplastic B cells in 432 B-CLPD patients, grouped according to diagnosis and site of sampling, versus their normal counterparts. Overall, proliferation of neoplastic B cells highly varied among the different B-CLPD subtypes, the greatest numbers of proliferating cells being identified in diffuse large B-cell lymphoma (DLBCL) and Burkitt lymphoma (BL). Compared with normal B cells, neoplastic B-CLPD cells showed significantly increased S + G2/M-phase values in mantle cell lymphoma (MCL), B-chronic lymphocytic leukemia (B-CLL), BL, and some DLBCL cases. Conversely, decreased proliferation was observed in follicular lymphoma, lymphoplasmacytic lymphoma/ Waldenstrom macroglobulinemia (LPL/ WM), and some DLBCL patients; hairy cell leukemia, splenic marginal zone, and MALT-lymphoma patients showed S + G 2/ M phase values similar to normal mature B lymphocytes from LN. Interestingly, in B-CLL and MCL significantly higher percentages of S + G 2/M cells were detected in BM versus PB and in LN versus BM and PB samples, respectively. In turn, presence of 14q32.3 gene rearrangements and DNA aneuploidy, was associated with a higher percentage of S + G2/M-phase cells among LPL/WM and B-CLL cases, respectively.
UR - http://www.scopus.com/inward/record.url?scp=46749101051&partnerID=8YFLogxK
U2 - 10.1182/blood-2007-10-119289
DO - 10.1182/blood-2007-10-119289
M3 - Article
C2 - 18337555
AN - SCOPUS:46749101051
SN - 0006-4971
VL - 111
SP - 5130
EP - 5141
JO - Blood
JF - Blood
IS - 10
ER -