TY - JOUR
T1 - Assessment of DNA damage in car spray painters exposed to organic solvents by the high-throughput comet assay
AU - Londoño-Velasco, Elizabeth
AU - Martínez-Perafán, Fabián
AU - Carvajal-Varona, Silvio
AU - García-Vallejo, Felipe
AU - Hoyos-Giraldo, Luz Stella
N1 - Publisher Copyright:
© 2016 Taylor & Francis.
PY - 2016/3/16
Y1 - 2016/3/16
N2 - Occupational exposure as a painter is associated with DNA damage and development of cancer. Comet assay has been widely adopted as a sensitive and quantitative tool for DNA damage assessment at the individual cell level in populations exposed to genotoxics. The aim of this study was to assess the application of the high-throughput comet assay, to determine the DNA damage in car spray painters. The study population included 52 car spray painters and 52 unexposed subjects. A significant increase in the %TDNA median (p5 0.001) was observed in the exposed group in comparison to the unexposed group. Neither age (%TDNA: p¼ 0.913) nor time of exposure (%TDNA: p¼0.398) were significantly correlated with DNA damage. The car spray painters who consumed alcohol did not show a significant increase in DNA damage compared to nonalcohol consumers (p 40.05). The results showed an increase in DNA breaks in car spray painters exposed to organic solvents and paints; furthermore, they demonstrated the application of high-throughput comet assay in an occupational exposure study to genotoxic agents.
AB - Occupational exposure as a painter is associated with DNA damage and development of cancer. Comet assay has been widely adopted as a sensitive and quantitative tool for DNA damage assessment at the individual cell level in populations exposed to genotoxics. The aim of this study was to assess the application of the high-throughput comet assay, to determine the DNA damage in car spray painters. The study population included 52 car spray painters and 52 unexposed subjects. A significant increase in the %TDNA median (p5 0.001) was observed in the exposed group in comparison to the unexposed group. Neither age (%TDNA: p¼ 0.913) nor time of exposure (%TDNA: p¼0.398) were significantly correlated with DNA damage. The car spray painters who consumed alcohol did not show a significant increase in DNA damage compared to nonalcohol consumers (p 40.05). The results showed an increase in DNA breaks in car spray painters exposed to organic solvents and paints; furthermore, they demonstrated the application of high-throughput comet assay in an occupational exposure study to genotoxic agents.
KW - Biomonitoring
KW - Car spray painters
KW - DNA damage
KW - High-throughput comet assay
KW - Occupational exposure
KW - Organic solvents
UR - http://www.scopus.com/inward/record.url?scp=84961390059&partnerID=8YFLogxK
U2 - 10.3109/15376516.2016.1158892
DO - 10.3109/15376516.2016.1158892
M3 - Article
C2 - 26998723
AN - SCOPUS:84961390059
SN - 1537-6516
VL - 26
SP - 238
EP - 242
JO - Toxicology Mechanisms and Methods
JF - Toxicology Mechanisms and Methods
IS - 4
ER -
