TY - JOUR
T1 - ANTAGONISTIC POTENTIAL OF S. SALIVARIUS, S. ORALIS AND S. DENTISANI ON DIFFERENT BIOTYPES OF S. MUTANS
AU - Gamboa Jaimes, Fredy Omar
AU - Plazas Cristancho, Leandro Augusto
AU - Lamby Tovar, Claudia Patricia
AU - Gamboa Vergara, Yalena Alicia
AU - Gómez, Olga Lucia
AU - Sarralde, Ana Lucia
AU - Sequeda Castañeda, Luis Gonzalo
N1 - Publisher Copyright:
© 2021, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.
PY - 2021/12/30
Y1 - 2021/12/30
N2 - Dental caries is an infectious process that results in tooth decay, where Streptococcus mutans is considered the main causing agent. An alternative which might prevent or control this disease is to identify microorganisms with S. mutans antagonistic potential. This study, encompassed within an Oral Microbial Ecology approach had as an objective to identify Streptococcus salivarius, Streptococcus oralis and Streptococcus dentisani strains with S. mutans antagonistic activity. Saliva samples were collected from 36 children without tooth decay and cultured on Lamb's Blood Agar and Mitis Salivarius Agar. After incubation at 37 ºC under aerobic atmosphere for 48 hours, S. salivarius, S. oralis and S. dentisani confirmed colonies were subjected to biochemical tests. To determine the antagonistic effect the double layer in agar technique was employed. Sixteen, 17 and 14 strains of S. salivarius, S. oralis and S. dentisani were identified representing a frequency of 44.4% (16/36), 47.2% (17/36) and 38.9% (14 / 36), respectively. S. salivarius, S. oralis and S. dentisani presented different modes of antagonistic action on S. mutans with inhibition halos from 6 to 15 mm. Only two strains, one from S. oralis and the other from S. dentisani, demonstrated complete antagonistic activity (100%) on the 12 S. mutans indicator strains. In conclusion, in the present study, 16, 17 and 14 strains of S. salivarius, S. oralis and S. dentisani, respectively with great antagonistic potential on different biotypes of S. mutans strains were identified, which after further characterization could be employed in strategies for the prevention or control of dental caries and other infections.
AB - Dental caries is an infectious process that results in tooth decay, where Streptococcus mutans is considered the main causing agent. An alternative which might prevent or control this disease is to identify microorganisms with S. mutans antagonistic potential. This study, encompassed within an Oral Microbial Ecology approach had as an objective to identify Streptococcus salivarius, Streptococcus oralis and Streptococcus dentisani strains with S. mutans antagonistic activity. Saliva samples were collected from 36 children without tooth decay and cultured on Lamb's Blood Agar and Mitis Salivarius Agar. After incubation at 37 ºC under aerobic atmosphere for 48 hours, S. salivarius, S. oralis and S. dentisani confirmed colonies were subjected to biochemical tests. To determine the antagonistic effect the double layer in agar technique was employed. Sixteen, 17 and 14 strains of S. salivarius, S. oralis and S. dentisani were identified representing a frequency of 44.4% (16/36), 47.2% (17/36) and 38.9% (14 / 36), respectively. S. salivarius, S. oralis and S. dentisani presented different modes of antagonistic action on S. mutans with inhibition halos from 6 to 15 mm. Only two strains, one from S. oralis and the other from S. dentisani, demonstrated complete antagonistic activity (100%) on the 12 S. mutans indicator strains. In conclusion, in the present study, 16, 17 and 14 strains of S. salivarius, S. oralis and S. dentisani, respectively with great antagonistic potential on different biotypes of S. mutans strains were identified, which after further characterization could be employed in strategies for the prevention or control of dental caries and other infections.
KW - Antagonism, S
KW - Dentisani, S
KW - Mutans
KW - Oralis, S
KW - Salivarius, S
UR - http://www.scopus.com/inward/record.url?scp=85130765172&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85130765172
SN - 1827-8620
VL - 3
SP - 1074
EP - 1084
JO - Pharmacologyonline
JF - Pharmacologyonline
ER -