Usefulness of IL-21, IL-7, and IL-15 conditioned media for expansion of antigen-specific CD8+ T cells from healthy donor-PBMCs suitable for immunotherapy

Julián A. Chamucero-Millares, David A. Bernal-Estévez, Carlos A. Parra-López

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Clonal anergy and depletion of antigen-specific CD8+ T cells are characteristics of immunosuppressed patients such as cancer and post-transplant patients. This has promoted translational research on the adoptive transfer of T cells to restore the antigen-specific cellular immunity in these patients. In the present work, we compared the capability of PBMCs and two types of mature monocyte-derived DCs (moDCs) to prime and to expand ex-vivo antigen-specific CD8+ T cells using culture conditioned media supplemented with IL-7, IL-15, and IL-21. The data obtained suggest that protocols involving moDCs are as efficient as PBMCs-based cultures in expanding antigen-specific CD8+ T cell to ELA and CMV model epitopes. These three gamma common chain cytokines promote the expansion of naïve-like and central memory CD8+ T cells in PBMCs-based cultures and the expansion of effector memory T cells when moDCs were used. Our results provide new insights into the use of media supplemented with IL-7, IL-15, and IL-21 for the in-vitro expansion of early-differentiated antigen-specific CD8+ T cells for immunotherapy purposes.

Original languageEnglish
Article number104257
JournalCellular Immunology
Volume360
DOIs
StatePublished - Feb 2021
Externally publishedYes

Keywords

  • Adoptive cell transfer
  • Antigen-specific T cells
  • Cancer immunotherapy
  • CD8 T cell
  • Dendritic cell-based immunotherapy
  • Immunological memory
  • Monocyte-derived dendritic cells
  • Viral immunity

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