Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells

D. Ballesteros-Vivas; Gerardo Alvarez-Rivera; Andrés Felipe García Ocampo; Sandra Johanna Morantes; Andrea del Pilar Sánchez Camargo; Alejandro Cifuentes; Fabián Parada-Alfonso; E. Ibánez

doi:10.1016/j.supflu.2019.104563

Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells

D. Ballesteros-Vivas
, Gerardo Alvarez-Rivera
, Andrés Felipe García Ocampo
, Sandra Johanna Morantes
, Andrea del Pilar Sánchez Camargo
, Alejandro Cifuentes
, Fabián Parada-Alfonso
, E. Ibánez

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Mango seed kernel (MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO₂ flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and 0.0625 feed/sc−CO₂ ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.

Original language	English
Article number	104563
Journal	Journal of Supercritical Fluids
Volume	152
DOIs	https://doi.org/10.1016/j.supflu.2019.104563
State	Published - Oct 2019
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antiproliferative activity
Fruit by-products
HT-29 colon cancer cell line
High-resolution mass spectrometry
LC-q-TOF-MS
Mangifera indica L
Pressurized-liquid extraction
Supercritical antisolvent fractionation

Access to Document

10.1016/j.supflu.2019.104563

Cite this

Ballesteros-Vivas, D., Alvarez-Rivera, G., García Ocampo, A. F., Morantes, S. J., Sánchez Camargo, A. D. P., Cifuentes, A., Parada-Alfonso, F., & Ibánez, E. (2019). Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells. Journal of Supercritical Fluids, 152, Article 104563. https://doi.org/10.1016/j.supflu.2019.104563

@article{a689f466bd6e4802b353b445ae017057,

title = "Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells",

abstract = "Mango seed kernel (MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14\%) was obtained in the extract using 50\% of water v/v in the feed and 0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.",

keywords = "Antiproliferative activity, Fruit by-products, HT-29 colon cancer cell line, High-resolution mass spectrometry, LC-q-TOF-MS, Mangifera indica L, Pressurized-liquid extraction, Supercritical antisolvent fractionation",

author = "D. Ballesteros-Vivas and Gerardo Alvarez-Rivera and \{Garc{\'i}a Ocampo\}, \{Andr{\'e}s Felipe\} and Morantes, \{Sandra Johanna\} and \{S{\'a}nchez Camargo\}, \{Andrea del Pilar\} and Alejandro Cifuentes and Fabi{\'a}n Parada-Alfonso and E. Ib{\'a}nez",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

year = "2019",

month = oct,

doi = "10.1016/j.supflu.2019.104563",

language = "English",

volume = "152",

journal = "Journal of Supercritical Fluids",

issn = "0896-8446",

publisher = "Elsevier B.V.",

}

Ballesteros-Vivas, D, Alvarez-Rivera, G, García Ocampo, AF, Morantes, SJ, Sánchez Camargo, ADP, Cifuentes, A, Parada-Alfonso, F & Ibánez, E 2019, 'Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells', Journal of Supercritical Fluids, vol. 152, 104563. https://doi.org/10.1016/j.supflu.2019.104563

TY - JOUR

T1 - Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells

AU - Ballesteros-Vivas, D.

AU - Alvarez-Rivera, Gerardo

AU - García Ocampo, Andrés Felipe

AU - Morantes, Sandra Johanna

AU - Sánchez Camargo, Andrea del Pilar

AU - Cifuentes, Alejandro

AU - Parada-Alfonso, Fabián

AU - Ibánez, E.

PY - 2019/10

Y1 - 2019/10

N2 - Mango seed kernel (MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and 0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.

AB - Mango seed kernel (MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and 0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.

KW - Antiproliferative activity

KW - Fruit by-products

KW - HT-29 colon cancer cell line

KW - High-resolution mass spectrometry

KW - LC-q-TOF-MS

KW - Mangifera indica L

KW - Pressurized-liquid extraction

KW - Supercritical antisolvent fractionation

UR - https://www.scopus.com/pages/publications/85068250476

U2 - 10.1016/j.supflu.2019.104563

DO - 10.1016/j.supflu.2019.104563

M3 - Article

AN - SCOPUS:85068250476

SN - 0896-8446

VL - 152

JO - Journal of Supercritical Fluids

JF - Journal of Supercritical Fluids

M1 - 104563

ER -

Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this