Sporozoite and Liver Stage Antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

Alvaro Puentes; Javier García; Ricardo Vera; Ramsés López; Jorge Suarez; Luis Rodríguez; Hernando Curtidor; Marisol Ocampo; Diana Tovar; Martha Forero; Adriana Bermudez; Jimena Cortes; Mauricio Urquiza; Manuel E. Patarroyo

doi:10.1016/j.vaccine.2003.09.029

Sporozoite and Liver Stage Antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

Alvaro Puentes, Javier García, Ricardo Vera, Ramsés López, Jorge Suarez, Luis Rodríguez, Hernando Curtidor, Marisol Ocampo, Diana Tovar, Martha Forero, Adriana Bermudez, Jimena Cortes, Mauricio Urquiza, Manuel E. Patarroyo

Universidad Nacional de Colombia

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 (²¹IWASEKKDEKEASEQGEESHY⁴⁰) and 20611 (⁶⁴KKDDGTDKVQEKVLEKSPKY⁸³) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes.

Original language	English
Pages (from-to)	1150-1156
Number of pages	7
Journal	Vaccine
Volume	22
Issue number	9-10
DOIs	https://doi.org/10.1016/j.vaccine.2003.09.029
State	Published - 12 Mar 2004
Externally published	Yes

Keywords

Binding peptides
CSP
Circumsporozoite protein
Critical residues
HABPs
High activity binding peptides
PBS
Phosphate buffered saline
Plasmodium falciparum
SALSA
SALSA protein
SSP-2
Sporozoite and Liver Stage Antigen
Sporozoite surface protein 2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2003.09.029

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Puentes, A., García, J., Vera, R., López, R., Suarez, J., Rodríguez, L., Curtidor, H., Ocampo, M., Tovar, D., Forero, M., Bermudez, A., Cortes, J., Urquiza, M., & Patarroyo, M. E. (2004). Sporozoite and Liver Stage Antigen Plasmodium falciparum peptides bind specifically to human hepatocytes. Vaccine, 22(9-10), 1150-1156. https://doi.org/10.1016/j.vaccine.2003.09.029

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title = "Sporozoite and Liver Stage Antigen Plasmodium falciparum peptides bind specifically to human hepatocytes",

abstract = "Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 (21IWASEKKDEKEASEQGEESHY40) and 20611 (64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes.",

keywords = "Binding peptides, CSP, Circumsporozoite protein, Critical residues, HABPs, High activity binding peptides, PBS, Phosphate buffered saline, Plasmodium falciparum, SALSA, SALSA protein, SSP-2, Sporozoite and Liver Stage Antigen, Sporozoite surface protein 2",

author = "Alvaro Puentes and Javier Garc{\'i}a and Ricardo Vera and Rams{\'e}s L{\'o}pez and Jorge Suarez and Luis Rodr{\'i}guez and Hernando Curtidor and Marisol Ocampo and Diana Tovar and Martha Forero and Adriana Bermudez and Jimena Cortes and Mauricio Urquiza and Patarroyo, {Manuel E.}",

note = "Funding Information: This study was supported by the President of Colombia{\textquoteright}s office and the Ministry of Public Health. Jason Garry{\textquoteright}s help in writing this manuscript is greatly appreciated. ",

year = "2004",

month = mar,

day = "12",

doi = "10.1016/j.vaccine.2003.09.029",

language = "English",

volume = "22",

pages = "1150--1156",

journal = "Vaccine",

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T1 - Sporozoite and Liver Stage Antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

AU - Puentes, Alvaro

AU - García, Javier

AU - Vera, Ricardo

AU - López, Ramsés

AU - Suarez, Jorge

AU - Rodríguez, Luis

AU - Curtidor, Hernando

AU - Ocampo, Marisol

AU - Tovar, Diana

AU - Forero, Martha

AU - Bermudez, Adriana

AU - Cortes, Jimena

AU - Urquiza, Mauricio

AU - Patarroyo, Manuel E.

N1 - Funding Information: This study was supported by the President of Colombia’s office and the Ministry of Public Health. Jason Garry’s help in writing this manuscript is greatly appreciated.

PY - 2004/3/12

Y1 - 2004/3/12

N2 - Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 (21IWASEKKDEKEASEQGEESHY40) and 20611 (64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes.

AB - Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 (21IWASEKKDEKEASEQGEESHY40) and 20611 (64KKDDGTDKVQEKVLEKSPKY83) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWSSMKMDEKMAAMQGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides' (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes.

KW - Binding peptides

KW - CSP

KW - Circumsporozoite protein

KW - Critical residues

KW - HABPs

KW - High activity binding peptides

KW - PBS

KW - Phosphate buffered saline

KW - Plasmodium falciparum

KW - SALSA

KW - SALSA protein

KW - SSP-2

KW - Sporozoite and Liver Stage Antigen

KW - Sporozoite surface protein 2

UR - http://www.scopus.com/inward/record.url?scp=10744230437&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2003.09.029

DO - 10.1016/j.vaccine.2003.09.029

M3 - Article

C2 - 15003642

AN - SCOPUS:10744230437

SN - 0264-410X

VL - 22

SP - 1150

EP - 1156

JO - Vaccine

JF - Vaccine

IS - 9-10

ER -

Sporozoite and Liver Stage Antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

Abstract

Keywords

UN SDGs

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