Sequence polymorphism in the Trypanosoma rangeli HSP70 coding genes allows typing of the parasite KP1(+) and KP1(-) groups

Claudia Cuervo; M. Carmen Thomas; Manuel C. López; Concepción J. Puerta

doi:10.1016/j.exppara.2013.01.001

Sequence polymorphism in the Trypanosoma rangeli HSP70 coding genes allows typing of the parasite KP1(+) and KP1(-) groups

Claudia Cuervo, M. Carmen Thomas, Manuel C. López, Concepción J. Puerta

Department of Microbiology

Consejo Sup. de Investigacions Cie.

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The genes encoding the Trypanosoma rangeli heat shock protein 70. kDa were sequenced and their genomic organization determined. This human parasite has medical relevance as it shares antigens, hosts and geographical regions with the etiological agent of Chagas' disease, Trypanosoma cruzi. The T. rangeli HSP70 genes are highly conserved regarding their tandem organization, and deduced amino acid sequences among T. rangeli KP1(+) and KP1(-) groups and other trypanosomatids. Nevertheless, a variable number of the immunogenic GMPG motif was observed among HSP70 copies within the same T. rangeli isolate and among different isolates. Interestingly, a polymorphism at nucleotide level affecting the SphI restriction site allowed the differentiation of KP1(-) and KP1(+) groups.

Original language	English
Pages (from-to)	447-453
Number of pages	7
Journal	Experimental Parasitology
Volume	133
Issue number	4
DOIs	https://doi.org/10.1016/j.exppara.2013.01.001
State	Published - Apr 2013

Keywords

HSP70 genes
SphI polymorphism
Trypanosoma rangeli

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.exppara.2013.01.001

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@article{274291a72f11452499c99ea203fc869c,

title = "Sequence polymorphism in the Trypanosoma rangeli HSP70 coding genes allows typing of the parasite KP1(+) and KP1(-) groups",

abstract = "The genes encoding the Trypanosoma rangeli heat shock protein 70. kDa were sequenced and their genomic organization determined. This human parasite has medical relevance as it shares antigens, hosts and geographical regions with the etiological agent of Chagas' disease, Trypanosoma cruzi. The T. rangeli HSP70 genes are highly conserved regarding their tandem organization, and deduced amino acid sequences among T. rangeli KP1(+) and KP1(-) groups and other trypanosomatids. Nevertheless, a variable number of the immunogenic GMPG motif was observed among HSP70 copies within the same T. rangeli isolate and among different isolates. Interestingly, a polymorphism at nucleotide level affecting the SphI restriction site allowed the differentiation of KP1(-) and KP1(+) groups.",

keywords = "HSP70 genes, SphI polymorphism, Trypanosoma rangeli",

author = "Claudia Cuervo and Thomas, {M. Carmen} and L{\'o}pez, {Manuel C.} and Puerta, {Concepci{\'o}n J.}",

note = "Funding Information: This work was supported by Vicerrectoria Academica, Pontificia Universidad Javeriana, project nr. 1060. Claudia Cuervo was supported by Colciencias (Colombian Institute for Development of Science and Technology) Scholarship programme for National Doctorate studies, call purpose in 2003. MC Thomas and MC L{\'o}pez were supported by Grants P08-CVI-04037PAI (Junta de Andaluc{\'i}a), BFU2010-1670 from Plan National I+D+i (MICINN), RD06/0021/0014 - ISCIII-RETIC (MICINN, Spain) and FEDER.",

year = "2013",

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language = "English",

volume = "133",

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journal = "Experimental Parasitology",

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AU - Cuervo, Claudia

AU - Thomas, M. Carmen

AU - López, Manuel C.

AU - Puerta, Concepción J.

N1 - Funding Information: This work was supported by Vicerrectoria Academica, Pontificia Universidad Javeriana, project nr. 1060. Claudia Cuervo was supported by Colciencias (Colombian Institute for Development of Science and Technology) Scholarship programme for National Doctorate studies, call purpose in 2003. MC Thomas and MC López were supported by Grants P08-CVI-04037PAI (Junta de Andalucía), BFU2010-1670 from Plan National I+D+i (MICINN), RD06/0021/0014 - ISCIII-RETIC (MICINN, Spain) and FEDER.

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AB - The genes encoding the Trypanosoma rangeli heat shock protein 70. kDa were sequenced and their genomic organization determined. This human parasite has medical relevance as it shares antigens, hosts and geographical regions with the etiological agent of Chagas' disease, Trypanosoma cruzi. The T. rangeli HSP70 genes are highly conserved regarding their tandem organization, and deduced amino acid sequences among T. rangeli KP1(+) and KP1(-) groups and other trypanosomatids. Nevertheless, a variable number of the immunogenic GMPG motif was observed among HSP70 copies within the same T. rangeli isolate and among different isolates. Interestingly, a polymorphism at nucleotide level affecting the SphI restriction site allowed the differentiation of KP1(-) and KP1(+) groups.

KW - HSP70 genes

KW - SphI polymorphism

KW - Trypanosoma rangeli

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JO - Experimental Parasitology

JF - Experimental Parasitology

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ER -

Sequence polymorphism in the Trypanosoma rangeli HSP70 coding genes allows typing of the parasite KP1(+) and KP1(-) groups

Abstract

Keywords

UN SDGs

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