Rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

Jingjing Liu; Ivona Loncar; J. Margriet Colleé; Manjeet K. Bolla; Joe Dennis; Kyriaki Michailidou; Qin Wang; Irene L. Andrulis; Monica Barile; Matthias W. Beckmann; Sabine Behrens; Javier Benitez; Carl Blomqvist; Bram Boeckx; Natalia V. Bogdanova; Stig E. Bojesen; Hiltrud Brauch; Paul Brennan; Hermann Brenner; Annegien Broeks; Barbara Burwinkel; Jenny Chang-Claude; Shou Tung Chen; Georgia Chenevix-Trench; Ching Y. Cheng; Ji Yeob Choi; Fergus J. Couch; Angela Cox; Simon S. Cross; Katarina Cuk; Kamila Czene; Thilo Dörk; Isabel Dos-Santos-Silva; Peter A. Fasching; Jonine Figueroa; Henrik Flyger; Montserrat Garciá-Closas; Graham G. Giles; Gord Glendon; Mark S. Goldberg; Anna González-Neira; Pascal Guénel; Christopher A. Haiman; Ute Hamann; Steven N. Hart; Mikael Hartman; Sigrid Hatse; John L. Hopper; Hidemi Ito; Anna Jakubowska; Maria Kabisch; Daehee Kang; Veli Matti Kosma; Vessela N. Kristensen; Loic Le Marchand; Eunjung Lee; Jingmei Li; Artitaya Lophatananon; Jan Lubinski; Arto Mannermaa; Keitaro Matsuo; Roger L. Milne; Susan L. Neuhausen; Heli Nevanlinna; Nick Orr; Jose I.A. Perez; Julian Peto; Thomas C. Putti; Katri Pylkäs; Paolo Radice; Suleeporn Sangrajrang; Elinor J. Sawyer; Marjanka K. Schmidt; Andreas Schneeweiss; Chen Yang Shen; Martha J. Shrubsole; Xiao Ou Shu; Jacques Simard; Melissa C. Southey; Anthony Swerdlow; Soo H. Teo; Daniel C. Tessier; Somchai Thanasitthichai; Ian Tomlinson; Diana Torres; Thérèse Truong; Chiu Chen Tseng; Celine Vachon; Robert Winqvist; Anna H. Wu; Drakoulis Yannoukakos; Wei Zheng; Per Hall; Alison M. Dunning; Douglas F. Easton; Maartje J. Hooning; Ans M.W. Van Den Ouweland; John W.M. Martens; Antoinette Hollestelle

doi:10.1038/srep36874

Rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

Jingjing Liu
, Ivona Loncar
, J. Margriet Colleé
, Manjeet K. Bolla
, Joe Dennis
, Kyriaki Michailidou
, Qin Wang
, Irene L. Andrulis
, Monica Barile
, Matthias W. Beckmann
, Sabine Behrens
, Javier Benitez
, Carl Blomqvist
, Bram Boeckx
, Natalia V. Bogdanova
, Stig E. Bojesen
, Hiltrud Brauch
, Paul Brennan
, Hermann Brenner
, Annegien Broeks

Barbara Burwinkel, Jenny Chang-Claude, Shou Tung Chen, Georgia Chenevix-Trench, Ching Y. Cheng, Ji Yeob Choi, Fergus J. Couch, Angela Cox, Simon S. Cross, Katarina Cuk, Kamila Czene, Thilo Dörk, Isabel Dos-Santos-Silva, Peter A. Fasching, Jonine Figueroa, Henrik Flyger, Montserrat Garciá-Closas, Graham G. Giles, Gord Glendon, Mark S. Goldberg, Anna González-Neira, Pascal Guénel, Christopher A. Haiman, Ute Hamann, Steven N. Hart, Mikael Hartman, Sigrid Hatse, John L. Hopper, Hidemi Ito, Anna Jakubowska, Maria Kabisch, Daehee Kang, Veli Matti Kosma, Vessela N. Kristensen, Loic Le Marchand, Eunjung Lee, Jingmei Li, Artitaya Lophatananon, Jan Lubinski, Arto Mannermaa, Keitaro Matsuo, Roger L. Milne, Susan L. Neuhausen, Heli Nevanlinna, Nick Orr, Jose I.A. Perez, Julian Peto, Thomas C. Putti, Katri Pylkäs, Paolo Radice, Suleeporn Sangrajrang, Elinor J. Sawyer, Marjanka K. Schmidt, Andreas Schneeweiss, Chen Yang Shen, Martha J. Shrubsole, Xiao Ou Shu, Jacques Simard, Melissa C. Southey, Anthony Swerdlow, Soo H. Teo, Daniel C. Tessier, Somchai Thanasitthichai, Ian Tomlinson, Diana Torres, Thérèse Truong, Chiu Chen Tseng, Celine Vachon, Robert Winqvist, Anna H. Wu, Drakoulis Yannoukakos, Wei Zheng, Per Hall, Alison M. Dunning, Douglas F. Easton, Maartje J. Hooning, Ans M.W. Van Den Ouweland, John W.M. Martens, Antoinette Hollestelle

Institute of Human Genetics

Erasmus MC Cancer Institute
Erasmus University Rotterdam
University of Cambridge
Cyprus Institute of Neurology and Genetics
University of Toronto
European Institute of Oncology
Friedrich-Alexander University Erlangen-Nürnberg
German Cancer Research Center
University of Hamburg
Centro de Investigación en Red de Enfermedades Raras
Centre for Biomedical Research on Rare Diseases (CIBERER)
Helsinki University Hospital
Flanders Institute for Biotechnology
KU Leuven
Hannover Medical School
The N.N. Alexandrov Research Institute of Oncology and Medical Radiology
Copenhagen University Hospital – Herlev and Gentofte
University of Copenhagen
Dr. Margarete Fischer-Bosch-Institute of Clinical Pharmacology
University of Tübingen
International Agency for Research on Cancer
Antoni van Leeuwenhoek Hospital
Heidelberg University
Changhua Christian Hospital
Queensland Institute of Medical Research
Singapore National Eye Center
National University of Singapore
Duke-NUS Medical School
Seoul National University
Mayo Clinic Rochester, MN
University of Sheffield
Karolinska Institutet
London School of Hygiene and Tropical Medicine
University of California at Los Angeles
University of Edinburgh
National Cancer Institute (NCI)
Cancer Council Victoria
Centre for Epidemiology and Biostatistics
McGill University
McGill University Health Centre, Royal Victoria Hospital
Occupational and Social Determinants of Health
Keck School of Medicine of USC
MOH Holdings Pte Ltd.
University Hospitals Leuven
Aichi Cancer Center Hospital and Research Institute
Nagoya University
Pomeranian Medical University in Szczecin
University of Eastern Finland
University of Oslo
University of Hawaii Cancer Center
University of Warwick
University of Manchester
City of Hope National Med Center
Institute of Cancer Research
Hospital Monte Naranco
University of Oulu
Northern Finland Laboratory Centre Oulu
Istituto di Ricovero e Cura a Carattere Scientifico
The National Cancer Institute
Guy’s Hospital
China Medical University Taichung
Academia Sinica - Institute of Biomedical Sciences
Vanderbilt University Medical Center
Université Laval
University of Melbourne
Cancer Research Malaysia
University of Malaya
University of Oxford
Demokritos National Centre for Scientific Research
Cancer Genomics Netherlands

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3′-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r 2 > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.

Original language	English
Article number	36874
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep36874
State	Published - 15 Nov 2016

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10.1038/srep36874

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Liu, J., Loncar, I., Colleé, J. M., Bolla, M. K., Dennis, J., Michailidou, K., Wang, Q., Andrulis, I. L., Barile, M., Beckmann, M. W., Behrens, S., Benitez, J., Blomqvist, C., Boeckx, B., Bogdanova, N. V., Bojesen, S. E., Brauch, H., Brennan, P., Brenner, H., ... Hollestelle, A. (2016). Rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk. Scientific Reports, 6, Article 36874. https://doi.org/10.1038/srep36874

@article{ebff2294603646108926f8c5736320fe,

title = "Rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk",

abstract = "NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3′-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95\% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r 2 > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95\% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95\% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.",

author = "Jingjing Liu and Ivona Loncar and Colle{\'e}, \{J. Margriet\} and Bolla, \{Manjeet K.\} and Joe Dennis and Kyriaki Michailidou and Qin Wang and Andrulis, \{Irene L.\} and Monica Barile and Beckmann, \{Matthias W.\} and Sabine Behrens and Javier Benitez and Carl Blomqvist and Bram Boeckx and Bogdanova, \{Natalia V.\} and Bojesen, \{Stig E.\} and Hiltrud Brauch and Paul Brennan and Hermann Brenner and Annegien Broeks and Barbara Burwinkel and Jenny Chang-Claude and Chen, \{Shou Tung\} and Georgia Chenevix-Trench and Cheng, \{Ching Y.\} and Choi, \{Ji Yeob\} and Couch, \{Fergus J.\} and Angela Cox and Cross, \{Simon S.\} and Katarina Cuk and Kamila Czene and Thilo D{\"o}rk and Isabel Dos-Santos-Silva and Fasching, \{Peter A.\} and Jonine Figueroa and Henrik Flyger and Montserrat Garci{\'a}-Closas and Giles, \{Graham G.\} and Gord Glendon and Goldberg, \{Mark S.\} and Anna Gonz{\'a}lez-Neira and Pascal Gu{\'e}nel and Haiman, \{Christopher A.\} and Ute Hamann and Hart, \{Steven N.\} and Mikael Hartman and Sigrid Hatse and Hopper, \{John L.\} and Hidemi Ito and Anna Jakubowska and Maria Kabisch and Daehee Kang and Kosma, \{Veli Matti\} and Kristensen, \{Vessela N.\} and \{Le Marchand\}, Loic and Eunjung Lee and Jingmei Li and Artitaya Lophatananon and Jan Lubinski and Arto Mannermaa and Keitaro Matsuo and Milne, \{Roger L.\} and Neuhausen, \{Susan L.\} and Heli Nevanlinna and Nick Orr and Perez, \{Jose I.A.\} and Julian Peto and Putti, \{Thomas C.\} and Katri Pylk{\"a}s and Paolo Radice and Suleeporn Sangrajrang and Sawyer, \{Elinor J.\} and Schmidt, \{Marjanka K.\} and Andreas Schneeweiss and Shen, \{Chen Yang\} and Shrubsole, \{Martha J.\} and Shu, \{Xiao Ou\} and Jacques Simard and Southey, \{Melissa C.\} and Anthony Swerdlow and Teo, \{Soo H.\} and Tessier, \{Daniel C.\} and Somchai Thanasitthichai and Ian Tomlinson and Diana Torres and Th{\'e}r{\`e}se Truong and Tseng, \{Chiu Chen\} and Celine Vachon and Robert Winqvist and Wu, \{Anna H.\} and Drakoulis Yannoukakos and Wei Zheng and Per Hall and Dunning, \{Alison M.\} and Easton, \{Douglas F.\} and Hooning, \{Maartje J.\} and \{Van Den Ouweland\}, \{Ans M.W.\} and Martens, \{John W.M.\} and Antoinette Hollestelle",

note = "Publisher Copyright: {\textcopyright} 2016 The Author(s).",

year = "2016",

month = nov,

day = "15",

doi = "10.1038/srep36874",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

}

Liu, J, Loncar, I, Colleé, JM, Bolla, MK, Dennis, J, Michailidou, K, Wang, Q, Andrulis, IL, Barile, M, Beckmann, MW, Behrens, S, Benitez, J, Blomqvist, C, Boeckx, B, Bogdanova, NV, Bojesen, SE, Brauch, H, Brennan, P, Brenner, H, Broeks, A, Burwinkel, B, Chang-Claude, J, Chen, ST, Chenevix-Trench, G, Cheng, CY, Choi, JY, Couch, FJ, Cox, A, Cross, SS, Cuk, K, Czene, K, Dörk, T, Dos-Santos-Silva, I, Fasching, PA, Figueroa, J, Flyger, H, Garciá-Closas, M, Giles, GG, Glendon, G, Goldberg, MS, González-Neira, A, Guénel, P, Haiman, CA, Hamann, U, Hart, SN, Hartman, M, Hatse, S, Hopper, JL, Ito, H, Jakubowska, A, Kabisch, M, Kang, D, Kosma, VM, Kristensen, VN, Le Marchand, L, Lee, E, Li, J, Lophatananon, A, Lubinski, J, Mannermaa, A, Matsuo, K, Milne, RL, Neuhausen, SL, Nevanlinna, H, Orr, N, Perez, JIA, Peto, J, Putti, TC, Pylkäs, K, Radice, P, Sangrajrang, S, Sawyer, EJ, Schmidt, MK, Schneeweiss, A, Shen, CY, Shrubsole, MJ, Shu, XO, Simard, J, Southey, MC, Swerdlow, A, Teo, SH, Tessier, DC, Thanasitthichai, S, Tomlinson, I, Torres, D, Truong, T, Tseng, CC, Vachon, C, Winqvist, R, Wu, AH, Yannoukakos, D, Zheng, W, Hall, P, Dunning, AM, Easton, DF, Hooning, MJ, Van Den Ouweland, AMW, Martens, JWM & Hollestelle, A 2016, 'Rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk', Scientific Reports, vol. 6, 36874. https://doi.org/10.1038/srep36874

TY - JOUR

T1 - Rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

AU - Liu, Jingjing

AU - Loncar, Ivona

AU - Colleé, J. Margriet

AU - Bolla, Manjeet K.

AU - Dennis, Joe

AU - Michailidou, Kyriaki

AU - Wang, Qin

AU - Andrulis, Irene L.

AU - Barile, Monica

AU - Beckmann, Matthias W.

AU - Behrens, Sabine

AU - Benitez, Javier

AU - Blomqvist, Carl

AU - Boeckx, Bram

AU - Bogdanova, Natalia V.

AU - Bojesen, Stig E.

AU - Brauch, Hiltrud

AU - Brennan, Paul

AU - Brenner, Hermann

AU - Broeks, Annegien

AU - Burwinkel, Barbara

AU - Chang-Claude, Jenny

AU - Chen, Shou Tung

AU - Chenevix-Trench, Georgia

AU - Cheng, Ching Y.

AU - Choi, Ji Yeob

AU - Couch, Fergus J.

AU - Cox, Angela

AU - Cross, Simon S.

AU - Cuk, Katarina

AU - Czene, Kamila

AU - Dörk, Thilo

AU - Dos-Santos-Silva, Isabel

AU - Fasching, Peter A.

AU - Figueroa, Jonine

AU - Flyger, Henrik

AU - Garciá-Closas, Montserrat

AU - Giles, Graham G.

AU - Glendon, Gord

AU - Goldberg, Mark S.

AU - González-Neira, Anna

AU - Guénel, Pascal

AU - Haiman, Christopher A.

AU - Hamann, Ute

AU - Hart, Steven N.

AU - Hartman, Mikael

AU - Hatse, Sigrid

AU - Hopper, John L.

AU - Ito, Hidemi

AU - Jakubowska, Anna

AU - Kabisch, Maria

AU - Kang, Daehee

AU - Kosma, Veli Matti

AU - Kristensen, Vessela N.

AU - Le Marchand, Loic

AU - Lee, Eunjung

AU - Li, Jingmei

AU - Lophatananon, Artitaya

AU - Lubinski, Jan

AU - Mannermaa, Arto

AU - Matsuo, Keitaro

AU - Milne, Roger L.

AU - Neuhausen, Susan L.

AU - Nevanlinna, Heli

AU - Orr, Nick

AU - Perez, Jose I.A.

AU - Peto, Julian

AU - Putti, Thomas C.

AU - Pylkäs, Katri

AU - Radice, Paolo

AU - Sangrajrang, Suleeporn

AU - Sawyer, Elinor J.

AU - Schmidt, Marjanka K.

AU - Schneeweiss, Andreas

AU - Shen, Chen Yang

AU - Shrubsole, Martha J.

AU - Shu, Xiao Ou

AU - Simard, Jacques

AU - Southey, Melissa C.

AU - Swerdlow, Anthony

AU - Teo, Soo H.

AU - Tessier, Daniel C.

AU - Thanasitthichai, Somchai

AU - Tomlinson, Ian

AU - Torres, Diana

AU - Truong, Thérèse

AU - Tseng, Chiu Chen

AU - Vachon, Celine

AU - Winqvist, Robert

AU - Wu, Anna H.

AU - Yannoukakos, Drakoulis

AU - Zheng, Wei

AU - Hall, Per

AU - Dunning, Alison M.

AU - Easton, Douglas F.

AU - Hooning, Maartje J.

AU - Van Den Ouweland, Ans M.W.

AU - Martens, John W.M.

AU - Hollestelle, Antoinette

PY - 2016/11/15

Y1 - 2016/11/15

N2 - NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3′-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r 2 > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.

AB - NBS1, also known as NBN, plays an important role in maintaining genomic stability. Interestingly, rs2735383 G > C, located in a microRNA binding site in the 3′-untranslated region (UTR) of NBS1, was shown to be associated with increased susceptibility to lung and colorectal cancer. However, the relation between rs2735383 and susceptibility to breast cancer is not yet clear. Therefore, we genotyped rs2735383 in 1,170 familial non-BRCA1/2 breast cancer cases and 1,077 controls using PCR-based restriction fragment length polymorphism (RFLP-PCR) analysis, but found no association between rs2735383CC and breast cancer risk (OR = 1.214, 95% CI = 0.936-1.574, P = 0.144). Because we could not exclude a small effect size due to a limited sample size, we further analyzed imputed rs2735383 genotypes (r 2 > 0.999) of 47,640 breast cancer cases and 46,656 controls from the Breast Cancer Association Consortium (BCAC). However, rs2735383CC was not associated with overall breast cancer risk in European (OR = 1.014, 95% CI = 0.969-1.060, P = 0.556) nor in Asian women (OR = 0.998, 95% CI = 0.905-1.100, P = 0.961). Subgroup analyses by age, age at menarche, age at menopause, menopausal status, number of pregnancies, breast feeding, family history and receptor status also did not reveal a significant association. This study therefore does not support the involvement of the genotype at NBS1 rs2735383 in breast cancer susceptibility.

UR - https://www.scopus.com/pages/publications/84995390280

U2 - 10.1038/srep36874

DO - 10.1038/srep36874

M3 - Article

C2 - 27845421

AN - SCOPUS:84995390280

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 36874

ER -

Rs2735383, located at a microRNA binding site in the 3'UTR of NBS1, is not associated with breast cancer risk

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