TY - JOUR
T1 - Plasmodium vivax Duffy binding protein peptides specifically bind to reticulocytes
AU - Ocampo, Marisol
AU - Vera, Ricardo
AU - Eduardo Rodriguez, Luis
AU - Curtidor, Hernando
AU - Urquiza, Mauricio
AU - Suarez, Jorge
AU - Garcia, Javier
AU - Puentes, Alvaro
AU - Lopez, Ramsés
AU - Trujillo, Mary
AU - Torres, Elizabeth
AU - Elkin Patarroyo, Manuel
N1 - Funding Information:
We are extremely grateful to Dr Chetan Chitnis, from the International Centre for Genetic Engineering and Biotechnology, New Delhi, for his kind and invaluable gift of recombinant PvRII and his suggestion for using it in mild conditions, which led to our successful results. This research project was supported by the Presidency of the Republic of Colombia, and the Ministry of Public Health. We thank Jason Garry for painstakingly reviewing the manuscript. The collaboration of Aurora Cortes B and our chemistry section is greatly appreciated.
PY - 2002
Y1 - 2002
N2 - Plasmodium vivax Duffy Binding Protein (Pv-DBP) is essential during merozoite invasion of reticulocytes. Reticulocyte binding region identification is important for understanding Pv-DBP reticulocyte recognition. Fifty 20 mer non-overlapping peptides, spanning Pv-DBP sequences, were tested in erythrocyte and reticulocyte binding assays. Ten HARBPs, mainly located in region II (Kd 50-130 nM), were High Activity Reticulocyte Binding Peptides (HARBPs); one bound to erythrocytes. Reticulocyte trypsin-, chymotrypsin- or neuraminidase- treatment affects HARBP binding differently, suggesting that these peptides have different reticulocyte-binding-sites. Some peptides bound to a Coomasie non-stainable 40 Kda band. Some HARBPs were able to block recombinant PvRII binding (Pv-DBP region II) to Duffy positive reticulocytes.
AB - Plasmodium vivax Duffy Binding Protein (Pv-DBP) is essential during merozoite invasion of reticulocytes. Reticulocyte binding region identification is important for understanding Pv-DBP reticulocyte recognition. Fifty 20 mer non-overlapping peptides, spanning Pv-DBP sequences, were tested in erythrocyte and reticulocyte binding assays. Ten HARBPs, mainly located in region II (Kd 50-130 nM), were High Activity Reticulocyte Binding Peptides (HARBPs); one bound to erythrocytes. Reticulocyte trypsin-, chymotrypsin- or neuraminidase- treatment affects HARBP binding differently, suggesting that these peptides have different reticulocyte-binding-sites. Some peptides bound to a Coomasie non-stainable 40 Kda band. Some HARBPs were able to block recombinant PvRII binding (Pv-DBP region II) to Duffy positive reticulocytes.
KW - Duffy binding protein
KW - HAEBP (High Activity Erythrocyte Binding Peptide)
KW - HARBP (High Activity Reticulocyte Binding Peptide)
KW - Plasmodium vivax
UR - http://www.scopus.com/inward/record.url?scp=0036155001&partnerID=8YFLogxK
U2 - 10.1016/S0196-9781(01)00574-5
DO - 10.1016/S0196-9781(01)00574-5
M3 - Article
C2 - 11814613
AN - SCOPUS:0036155001
SN - 0196-9781
VL - 23
SP - 13
EP - 22
JO - Peptides
JF - Peptides
IS - 1
ER -