Plasmodium falciparum merozoite surface protein 6 (MSP-6) derived peptides bind erythrocytes and partially inhibit parasite invasion

Ramsés López; John Valbuena; Luis E. Rodríguez; Marisol Ocampo; Ricardo Vera; Hernando Curtidor; Alvaro Puentes; Javier García; Luis E. Ramirez; Manuel E. Patarroyo

doi:10.1016/j.peptides.2006.02.001

Plasmodium falciparum merozoite surface protein 6 (MSP-6) derived peptides bind erythrocytes and partially inhibit parasite invasion

Ramsés López
, John Valbuena
, Luis E. Rodríguez
, Marisol Ocampo
, Ricardo Vera
, Hernando Curtidor
, Alvaro Puentes
, Javier García
, Luis E. Ramirez
, Manuel E. Patarroyo

Fundación Instituto de Inmunología de Colombia

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

This work shows that Plasmodium falciparum merozoite surface protein-6 (MSP-6) peptides specifically bind to membrane surface receptor on human erythrocytes. Three high activity binding peptides (HABPs) were found: peptides 31175 (⁴¹MYNNDKILSKNEVDTNIESN⁶⁰) and 31178 (¹⁰¹YDIQATYQFPSTSGGNNVIP¹²⁰) in the amino terminal region and 31191 (³⁶¹EIDSTINNLVQEMIHLFSNNY³⁸⁰) at the carboxy terminal. Their binding to erythrocytes was saturable. HABPs 31191 and 31178 recognized 56 and 26 kDa receptors on erythrocyte membrane and inhibited in vitro Plasmodium falciparum merozoite invasion of erythrocytes by between 27% and 46% at 200 μg ml^-1 concentration, suggesting that these MSP-6 protein peptides play a possible role in the invasion process.

Original language	English
Pages (from-to)	1685-1692
Number of pages	8
Journal	Peptides
Volume	27
Issue number	7
DOIs	https://doi.org/10.1016/j.peptides.2006.02.001
State	Published - Jul 2006
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Binding
Invasion
Ligand
MSP-6
Plasmodium falciparum
Receptor
Vaccine

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10.1016/j.peptides.2006.02.001

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@article{0ac947544dc748368b002dc92ca47731,

title = "Plasmodium falciparum merozoite surface protein 6 (MSP-6) derived peptides bind erythrocytes and partially inhibit parasite invasion",

abstract = "This work shows that Plasmodium falciparum merozoite surface protein-6 (MSP-6) peptides specifically bind to membrane surface receptor on human erythrocytes. Three high activity binding peptides (HABPs) were found: peptides 31175 (41MYNNDKILSKNEVDTNIESN60) and 31178 (101YDIQATYQFPSTSGGNNVIP120) in the amino terminal region and 31191 (361EIDSTINNLVQEMIHLFSNNY380) at the carboxy terminal. Their binding to erythrocytes was saturable. HABPs 31191 and 31178 recognized 56 and 26 kDa receptors on erythrocyte membrane and inhibited in vitro Plasmodium falciparum merozoite invasion of erythrocytes by between 27\% and 46\% at 200 μg ml-1 concentration, suggesting that these MSP-6 protein peptides play a possible role in the invasion process.",

keywords = "Binding, Invasion, Ligand, MSP-6, Plasmodium falciparum, Receptor, Vaccine",

author = "Rams{\'e}s L{\'o}pez and John Valbuena and Rodr{\'i}guez, \{Luis E.\} and Marisol Ocampo and Ricardo Vera and Hernando Curtidor and Alvaro Puentes and Javier Garc{\'i}a and Ramirez, \{Luis E.\} and Patarroyo, \{Manuel E.\}",

note = "Funding Information: This research project was supported by the President of Colombia's Office and the Colombian Ministry of Public Health.",

year = "2006",

month = jul,

doi = "10.1016/j.peptides.2006.02.001",

language = "English",

volume = "27",

pages = "1685--1692",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "7",

}

TY - JOUR

T1 - Plasmodium falciparum merozoite surface protein 6 (MSP-6) derived peptides bind erythrocytes and partially inhibit parasite invasion

AU - López, Ramsés

AU - Valbuena, John

AU - Rodríguez, Luis E.

AU - Ocampo, Marisol

AU - Vera, Ricardo

AU - Curtidor, Hernando

AU - Puentes, Alvaro

AU - García, Javier

AU - Ramirez, Luis E.

AU - Patarroyo, Manuel E.

N1 - Funding Information: This research project was supported by the President of Colombia's Office and the Colombian Ministry of Public Health.

PY - 2006/7

Y1 - 2006/7

N2 - This work shows that Plasmodium falciparum merozoite surface protein-6 (MSP-6) peptides specifically bind to membrane surface receptor on human erythrocytes. Three high activity binding peptides (HABPs) were found: peptides 31175 (41MYNNDKILSKNEVDTNIESN60) and 31178 (101YDIQATYQFPSTSGGNNVIP120) in the amino terminal region and 31191 (361EIDSTINNLVQEMIHLFSNNY380) at the carboxy terminal. Their binding to erythrocytes was saturable. HABPs 31191 and 31178 recognized 56 and 26 kDa receptors on erythrocyte membrane and inhibited in vitro Plasmodium falciparum merozoite invasion of erythrocytes by between 27% and 46% at 200 μg ml-1 concentration, suggesting that these MSP-6 protein peptides play a possible role in the invasion process.

AB - This work shows that Plasmodium falciparum merozoite surface protein-6 (MSP-6) peptides specifically bind to membrane surface receptor on human erythrocytes. Three high activity binding peptides (HABPs) were found: peptides 31175 (41MYNNDKILSKNEVDTNIESN60) and 31178 (101YDIQATYQFPSTSGGNNVIP120) in the amino terminal region and 31191 (361EIDSTINNLVQEMIHLFSNNY380) at the carboxy terminal. Their binding to erythrocytes was saturable. HABPs 31191 and 31178 recognized 56 and 26 kDa receptors on erythrocyte membrane and inhibited in vitro Plasmodium falciparum merozoite invasion of erythrocytes by between 27% and 46% at 200 μg ml-1 concentration, suggesting that these MSP-6 protein peptides play a possible role in the invasion process.

KW - Binding

KW - Invasion

KW - Ligand

KW - MSP-6

KW - Plasmodium falciparum

KW - Receptor

KW - Vaccine

UR - https://www.scopus.com/pages/publications/33744987635

U2 - 10.1016/j.peptides.2006.02.001

DO - 10.1016/j.peptides.2006.02.001

M3 - Article

C2 - 16713025

AN - SCOPUS:33744987635

SN - 0196-9781

VL - 27

SP - 1685

EP - 1692

JO - Peptides

JF - Peptides

IS - 7

ER -

Plasmodium falciparum merozoite surface protein 6 (MSP-6) derived peptides bind erythrocytes and partially inhibit parasite invasion

Abstract

UN SDGs

Keywords

Access to Document

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