Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Javier E. García; Alvaro Puentes; Ramsés López; Ricardo Vera; Jorge Suárez; Luis Rodríguez; Hernando Curtidor; Marisol Ocampo; Diana Tovar; Martha Forero; Adriana Bermudez; Jimena Cortés; Mauricio Urquiza; Manuel E. Patarroyo

doi:10.1016/S0196-9781(03)00135-9

Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Javier E. García
, Alvaro Puentes
, Ramsés López
, Ricardo Vera
, Jorge Suárez
, Luis Rodríguez
, Hernando Curtidor
, Marisol Ocampo
, Diana Tovar
, Martha Forero
, Adriana Bermudez
, Jimena Cortés
, Mauricio Urquiza
, Manuel E. Patarroyo

Universidad Nacional de Colombia

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (²¹INGKIIKNSEKDEIIKSNLRY⁴⁰), 20637 (¹⁵⁷KEKLQGQQSDSEQERRAY¹⁷³), 20638 (¹⁷⁴KEKLQEQQSDLEQERLAY¹⁹⁰) and 20639 (¹⁹¹KEKLQEQQSDLEQERRAY²⁰⁷) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (²¹INGKIIKNSEKDEIIKSNLRY⁴⁰) and 20633 (⁸¹DKELTMSNVKNVSQTNFKSLY¹⁰⁰) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

Original language	English
Pages (from-to)	647-657
Number of pages	11
Journal	Peptides
Volume	24
Issue number	5
DOIs	https://doi.org/10.1016/S0196-9781(03)00135-9
State	Published - 01 May 2003
Externally published	Yes

Keywords

Critical residues
High activity binding peptides (HABPs)
Liver stage antigen (LSA)
P. falciparum
Phosphate buffered saline (PBS)
Red blood cells (RBC)

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10.1016/S0196-9781(03)00135-9

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@article{1d0aae9c9c814ee9ac762482ff93e4b1,

title = "Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes",

abstract = "Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40), 20637 (157KEKLQGQQSDSEQERRAY173), 20638 (174KEKLQEQQSDLEQERLAY190) and 20639 (191KEKLQEQQSDLEQERRAY207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40) and 20633 (81DKELTMSNVKNVSQTNFKSLY100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 {"}High Activity Binding Peptides{"} could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.",

keywords = "Critical residues, High activity binding peptides (HABPs), Liver stage antigen (LSA), P. falciparum, Phosphate buffered saline (PBS), Red blood cells (RBC)",

author = "Garc{\'i}a, \{Javier E.\} and Alvaro Puentes and Rams{\'e}s L{\'o}pez and Ricardo Vera and Jorge Su{\'a}rez and Luis Rodr{\'i}guez and Hernando Curtidor and Marisol Ocampo and Diana Tovar and Martha Forero and Adriana Bermudez and Jimena Cort{\'e}s and Mauricio Urquiza and Patarroyo, \{Manuel E.\}",

year = "2003",

month = may,

day = "1",

doi = "10.1016/S0196-9781(03)00135-9",

language = "English",

volume = "24",

pages = "647--657",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "5",

}

TY - JOUR

T1 - Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

AU - García, Javier E.

AU - Puentes, Alvaro

AU - López, Ramsés

AU - Vera, Ricardo

AU - Suárez, Jorge

AU - Rodríguez, Luis

AU - Curtidor, Hernando

AU - Ocampo, Marisol

AU - Tovar, Diana

AU - Forero, Martha

AU - Bermudez, Adriana

AU - Cortés, Jimena

AU - Urquiza, Mauricio

AU - Patarroyo, Manuel E.

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40), 20637 (157KEKLQGQQSDSEQERRAY173), 20638 (174KEKLQEQQSDLEQERLAY190) and 20639 (191KEKLQEQQSDLEQERRAY207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40) and 20633 (81DKELTMSNVKNVSQTNFKSLY100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

AB - Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40), 20637 (157KEKLQGQQSDSEQERRAY173), 20638 (174KEKLQEQQSDLEQERLAY190) and 20639 (191KEKLQEQQSDLEQERRAY207) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 (21INGKIIKNSEKDEIIKSNLRY40) and 20633 (81DKELTMSNVKNVSQTNFKSLY100) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

KW - Critical residues

KW - High activity binding peptides (HABPs)

KW - Liver stage antigen (LSA)

KW - P. falciparum

KW - Phosphate buffered saline (PBS)

KW - Red blood cells (RBC)

UR - https://www.scopus.com/pages/publications/12444344702

U2 - 10.1016/S0196-9781(03)00135-9

DO - 10.1016/S0196-9781(03)00135-9

M3 - Article

C2 - 12895649

AN - SCOPUS:12444344702

SN - 0196-9781

VL - 24

SP - 647

EP - 657

JO - Peptides

JF - Peptides

IS - 5

ER -

Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Abstract

Keywords

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