TY - JOUR
T1 - Passiflora manicata (Juss.) aqueous leaf extract protects against reactive oxygen species and protein glycation in vitro and ex vivo models
AU - Da Silva Morrone, Maurilio
AU - De Assis, Adriano Martimbianco
AU - Da Rocha, Ricardo Fagundes
AU - Gasparotto, Juciano
AU - Gazola, Andressa Córneo
AU - Costa, Geison Modesti
AU - Zucolotto, Silvana Maria
AU - Castellanos, Leonardo H.
AU - Ramos, Freddy A.
AU - Schenkel, Eloir Paulo
AU - Reginatto, Flávio Henrique
AU - Gelain, Daniel Pens
AU - Moreira, José C.F.
N1 - Funding Information:
The Brazilian research funding agencies FAPERGS (PqG 1008860, PqG 1008857, ARD 11/1893-7, PRONEX 1000274) CAPES (PROCAD 066/2007), CNPq and PROPESQ UFRGS supported this study.
PY - 2013/10
Y1 - 2013/10
N2 - The leaf extracts of many species of genus Passiflora have been extensively investigated for their biological activities on several rat tissues, but mainly in the central nervous system and liver. They posses anxiolytic-like, sedative effects and antioxidant properties. Evidences suggest a key role of C-glycosylflavonoids in the biological activities of Passiflora extracts. Some species (such as P. manicata) of the genus are still poorly investigated for their chemical and biological activity. In this work, we aim to investigate both antioxidant and antiglycation properties of aqueous extract of P. manicata leaves (PMLE) in vitro and ex vivo models. Crude extract showed the C-glycosylflavonoid isovitexin as the major compound. Isoorientin and vitexin were also identified. In TRAP/TAR assay, PMLE showed a significant antioxidant activity. PMLE at concentrations of 10 and 100μgmL-1 significantly decreasing LDH leakage in rat liver slices. Antioxidant effect also was observed by decreased in oxidative damage markers in slices hence hydrogen peroxide was added as oxidative stress inductor. PMLE inhibited protein glycation at all concentrations tested. In summary, P. manicata aqueous leaf extract possess protective properties against reactive oxygen species and also protein glycation, and could be considered a new source of natural antioxidants.
AB - The leaf extracts of many species of genus Passiflora have been extensively investigated for their biological activities on several rat tissues, but mainly in the central nervous system and liver. They posses anxiolytic-like, sedative effects and antioxidant properties. Evidences suggest a key role of C-glycosylflavonoids in the biological activities of Passiflora extracts. Some species (such as P. manicata) of the genus are still poorly investigated for their chemical and biological activity. In this work, we aim to investigate both antioxidant and antiglycation properties of aqueous extract of P. manicata leaves (PMLE) in vitro and ex vivo models. Crude extract showed the C-glycosylflavonoid isovitexin as the major compound. Isoorientin and vitexin were also identified. In TRAP/TAR assay, PMLE showed a significant antioxidant activity. PMLE at concentrations of 10 and 100μgmL-1 significantly decreasing LDH leakage in rat liver slices. Antioxidant effect also was observed by decreased in oxidative damage markers in slices hence hydrogen peroxide was added as oxidative stress inductor. PMLE inhibited protein glycation at all concentrations tested. In summary, P. manicata aqueous leaf extract possess protective properties against reactive oxygen species and also protein glycation, and could be considered a new source of natural antioxidants.
KW - Antioxidant
KW - C-glycosylflavonoids
KW - Oxidative stress
KW - Passiflora manicata
KW - Protein glycation
UR - http://www.scopus.com/inward/record.url?scp=84881523567&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2013.07.028
DO - 10.1016/j.fct.2013.07.028
M3 - Article
C2 - 23876820
AN - SCOPUS:84881523567
SN - 0278-6915
VL - 60
SP - 45
EP - 51
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
ER -