Mortality of the Prickly Pear Cactus Weevil Metamasisus spinolae1 with the Application of Heterorhabditis bacteriophora2 JAAN-19

Ismael Fernando Chávez-Díaz; Adriana Sáenz-Aponte; Ricardo Vicente-Pérez; Alfonso Luna-Cruz; Petra Andrade-Hoyos; Carmela Hernández-Domínguez; Marco Aurelio Aragón-Magadan; Pedro Fabián Grifaldo-Alcántara

doi:10.3958/059.050.0214

Mortality of the Prickly Pear Cactus Weevil Metamasisus spinolae1 with the Application of Heterorhabditis bacteriophora2 JAAN-19

Ismael Fernando Chávez-Díaz
, Adriana Sáenz-Aponte
, Ricardo Vicente-Pérez
, Alfonso Luna-Cruz
, Petra Andrade-Hoyos
, Carmela Hernández-Domínguez
, Marco Aurelio Aragón-Magadan
, Pedro Fabián Grifaldo-Alcántara

UNIT OF ECOLOGY AND SYSTEMATICS (UNESIS)

Laboratorio de ADN y Genómica en el Centro Nacional de Recursos Genéticos Microbianos-INIFAP
Universidad de Guadalajara
Universidad Michoacana de San Nicolas de Hidalgo
Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias
Benemerita Universidad Autonoma de Puebla

Research output: Contribution to journal › Article › peer-review

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Abstract

We tested the nematode Heterorhabditis bacteriophora (Poinar) JAAN-19 as a biocontrol agent of Metamasius spinolae. A concentration of 1000 Jl/ weevil in vitro achieved 90% mortality in the females and males after 6 days. These results demonstrated the strong searching and infective capacity of the nematode, which resulted 97% mortality within 6-8 days. The lethal dose (LD₅₀) was calculated at 138 Jl/weevil, and the LD₉₀ was 463 Jl/weevil 6-8 days later. These findings strongly suggest that H. bacteriophora JAAN-19 can be used to control M. spinolae.

Translated title of the contribution	Mortalidad del picudo del nopal Metamasisus spinolae con la aplicación de Heterorhabditis bacteriophora JAAN-19
Original language	English
Pages (from-to)	1-15
Number of pages	15
Journal	Southwestern Entomologist
Volume	50
Issue number	2
DOIs	https://doi.org/10.3958/059.050.0214
State	Published - 2025

Keywords

Curculionidae
Opuntia
entomopathogenic nematode
mortality
virulence

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Chávez-Díaz, I. F., Sáenz-Aponte, A., Vicente-Pérez, R., Luna-Cruz, A., Andrade-Hoyos, P., Hernández-Domínguez, C., Aragón-Magadan, M. A., & Grifaldo-Alcántara, P. F. (2025). Mortality of the Prickly Pear Cactus Weevil Metamasisus spinolae1 with the Application of Heterorhabditis bacteriophora2 JAAN-19. Southwestern Entomologist, 50(2), 1-15. https://doi.org/10.3958/059.050.0214

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title = "Mortality of the Prickly Pear Cactus Weevil Metamasisus spinolae1 with the Application of Heterorhabditis bacteriophora2 JAAN-19",

abstract = "We tested the nematode Heterorhabditis bacteriophora (Poinar) JAAN-19 as a biocontrol agent of Metamasius spinolae. A concentration of 1000 Jl/ weevil in vitro achieved 90\% mortality in the females and males after 6 days. These results demonstrated the strong searching and infective capacity of the nematode, which resulted 97\% mortality within 6-8 days. The lethal dose (LD50) was calculated at 138 Jl/weevil, and the LD90 was 463 Jl/weevil 6-8 days later. These findings strongly suggest that H. bacteriophora JAAN-19 can be used to control M. spinolae.",

keywords = "Curculionidae, Opuntia, entomopathogenic nematode, mortality, virulence",

author = "Ch{\'a}vez-D{\'i}az, \{Ismael Fernando\} and Adriana S{\'a}enz-Aponte and Ricardo Vicente-P{\'e}rez and Alfonso Luna-Cruz and Petra Andrade-Hoyos and Carmela Hern{\'a}ndez-Dom{\'i}nguez and Arag{\'o}n-Magadan, \{Marco Aurelio\} and Grifaldo-Alc{\'a}ntara, \{Pedro Fabi{\'a}n\}",

year = "2025",

doi = "10.3958/059.050.0214",

language = "English",

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Chávez-Díaz, IF, Sáenz-Aponte, A, Vicente-Pérez, R, Luna-Cruz, A, Andrade-Hoyos, P, Hernández-Domínguez, C, Aragón-Magadan, MA & Grifaldo-Alcántara, PF 2025, 'Mortality of the Prickly Pear Cactus Weevil Metamasisus spinolae1 with the Application of Heterorhabditis bacteriophora2 JAAN-19', Southwestern Entomologist, vol. 50, no. 2, pp. 1-15. https://doi.org/10.3958/059.050.0214

TY - JOUR

T1 - Mortality of the Prickly Pear Cactus Weevil Metamasisus spinolae1 with the Application of Heterorhabditis bacteriophora2 JAAN-19

AU - Chávez-Díaz, Ismael Fernando

AU - Sáenz-Aponte, Adriana

AU - Vicente-Pérez, Ricardo

AU - Luna-Cruz, Alfonso

AU - Andrade-Hoyos, Petra

AU - Hernández-Domínguez, Carmela

AU - Aragón-Magadan, Marco Aurelio

AU - Grifaldo-Alcántara, Pedro Fabián

PY - 2025

Y1 - 2025

N2 - We tested the nematode Heterorhabditis bacteriophora (Poinar) JAAN-19 as a biocontrol agent of Metamasius spinolae. A concentration of 1000 Jl/ weevil in vitro achieved 90% mortality in the females and males after 6 days. These results demonstrated the strong searching and infective capacity of the nematode, which resulted 97% mortality within 6-8 days. The lethal dose (LD50) was calculated at 138 Jl/weevil, and the LD90 was 463 Jl/weevil 6-8 days later. These findings strongly suggest that H. bacteriophora JAAN-19 can be used to control M. spinolae.

AB - We tested the nematode Heterorhabditis bacteriophora (Poinar) JAAN-19 as a biocontrol agent of Metamasius spinolae. A concentration of 1000 Jl/ weevil in vitro achieved 90% mortality in the females and males after 6 days. These results demonstrated the strong searching and infective capacity of the nematode, which resulted 97% mortality within 6-8 days. The lethal dose (LD50) was calculated at 138 Jl/weevil, and the LD90 was 463 Jl/weevil 6-8 days later. These findings strongly suggest that H. bacteriophora JAAN-19 can be used to control M. spinolae.

KW - Curculionidae

KW - Opuntia

KW - entomopathogenic nematode

KW - mortality

KW - virulence

UR - https://www.scopus.com/pages/publications/105029538398

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DO - 10.3958/059.050.0214

M3 - Article

AN - SCOPUS:105029538398

SN - 0147-1724

VL - 50

SP - 1

EP - 15

JO - Southwestern Entomologist

JF - Southwestern Entomologist

IS - 2

ER -

Mortality of the Prickly Pear Cactus Weevil Metamasisus spinolae1 with the Application of Heterorhabditis bacteriophora2 JAAN-19

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