TY - JOUR
T1 - Molecular Detection and Genotyping of Cryptosporidium spp. Isolates from Bats in Colombia
AU - Silva-Ramos, Carlos Ramiro
AU - Noriega, Juliana
AU - Fajardo, Rafael F.
AU - Chala-Quintero, Sandra M.
AU - Del Pilar Pulido-Villamarín, Adriana
AU - Pérez-Torres, Jairo
AU - Castañeda-Salazar, Rubiela
AU - Cuervo, Claudia
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/8/2
Y1 - 2023/8/2
N2 - Purpose: Cryptosporidiosis is a zoonotic infectious disease caused by the protozoan parasite Cryptosporidium spp., frequently found in several animal species, including bats. Several Cryptosporidium genotypes have been described in bats worldwide, suggesting that bats are infected by host-specific Cryptosporidium spp. To date, there are no published reports about Cryptosporidium spp. in bats from Colombia. Therefore, this study aimed to determine the presence and molecular diversity of Cryptosporidium spp. in Colombian bats. Methods: A total of 63 gut samples from three bat species served for molecular detection of Cryptosporidium spp. 18S rDNA gene by qPCR. The sequenced amplicons were used in subsequent phylogenetic analyses to identify them as species or genotypes. Results: Cryptosporidium spp. qPCR detection occurred in 9.5% (6/63) of bat intestines, and four sequences represented two new genotypes, called Cryptosporidium bat genotypes XIX and XX, were identified. Conclusions: This study describes the detection of two novel Cryptosporidium bat genotypes, in two species of bats from a region of Colombia, requiring further studies to determine the relationhip between Cryptosporidium and bats in Colombia.
AB - Purpose: Cryptosporidiosis is a zoonotic infectious disease caused by the protozoan parasite Cryptosporidium spp., frequently found in several animal species, including bats. Several Cryptosporidium genotypes have been described in bats worldwide, suggesting that bats are infected by host-specific Cryptosporidium spp. To date, there are no published reports about Cryptosporidium spp. in bats from Colombia. Therefore, this study aimed to determine the presence and molecular diversity of Cryptosporidium spp. in Colombian bats. Methods: A total of 63 gut samples from three bat species served for molecular detection of Cryptosporidium spp. 18S rDNA gene by qPCR. The sequenced amplicons were used in subsequent phylogenetic analyses to identify them as species or genotypes. Results: Cryptosporidium spp. qPCR detection occurred in 9.5% (6/63) of bat intestines, and four sequences represented two new genotypes, called Cryptosporidium bat genotypes XIX and XX, were identified. Conclusions: This study describes the detection of two novel Cryptosporidium bat genotypes, in two species of bats from a region of Colombia, requiring further studies to determine the relationhip between Cryptosporidium and bats in Colombia.
KW - Bats
KW - Colombia
KW - Cryptosporidium
KW - Molecular detection
KW - Animals
KW - Cryptosporidiosis/parasitology
KW - Feces/parasitology
KW - Genotype
KW - Phylogeny
KW - Colombia/epidemiology
KW - Chiroptera/parasitology
KW - Cryptosporidium/genetics
UR - http://www.scopus.com/inward/record.url?scp=85166765447&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/357b1634-2183-3dea-91eb-18cf8a8b60e0/
U2 - 10.1007/s11686-023-00697-8
DO - 10.1007/s11686-023-00697-8
M3 - Article
C2 - 37531008
AN - SCOPUS:85166765447
SN - 1230-2821
VL - 68
SP - 676
EP - 682
JO - Acta Parasitologica
JF - Acta Parasitologica
IS - 3
ER -
