TY - GEN
T1 - MALDI-TOF MS for the identification of bacteria from Acropora cervicornis samples with White Band Disease
AU - Fennix-Agudelo, Mary Andrea
AU - Sánchez-Quitian, Zilpa Adriana
AU - Parra-Giraldo, Claudia Marcela
AU - Zarza-González, Esteban
AU - Merchán-Castellanos, Nuri
AU - Chavarro-Mesa, Edisson
AU - Martinez-Santos, Juan Carlos
N1 - Publisher Copyright:
© 2022 Latin American and Caribbean Consortium of Engineering Institutions. All rights reserved.
PY - 2022
Y1 - 2022
N2 - White Band Disease (WBD) is one of the most harmful coral syndromes in the Caribbean, deteriorating the structure of many corals during the eighties and nineties. Since its etiology is not entirely known, the use of techniques aimed a t identifying the associated agents could provide an approach to the composition of the populations involved in the deterioration of A. cervicornis. Therefore, this research's objective was to isolate and characterize bacteria associated with WBD in A. cervicornis from hanging nurseries of Rosario and San Bernardo Corals National Natural Park, Colombia, using MALDI-TO F MS and PCR. The results showed the presence of Bacillus cereus bacteria in individuals with bleaching signs from nurseries and transplant areas on Isla Tesoro and Isla Ceiner. One of these strains sequenced and phylogenetically analyzed was closely related t o Bacillus thuringiensis, with similarities of up to 99%. The presence of B. cereus could indicate a special relationship between this microorganism and WBD. However, it is imperative to carry out more studies on identifying the bacteria associated with this disease to support these findings.
AB - White Band Disease (WBD) is one of the most harmful coral syndromes in the Caribbean, deteriorating the structure of many corals during the eighties and nineties. Since its etiology is not entirely known, the use of techniques aimed a t identifying the associated agents could provide an approach to the composition of the populations involved in the deterioration of A. cervicornis. Therefore, this research's objective was to isolate and characterize bacteria associated with WBD in A. cervicornis from hanging nurseries of Rosario and San Bernardo Corals National Natural Park, Colombia, using MALDI-TO F MS and PCR. The results showed the presence of Bacillus cereus bacteria in individuals with bleaching signs from nurseries and transplant areas on Isla Tesoro and Isla Ceiner. One of these strains sequenced and phylogenetically analyzed was closely related t o Bacillus thuringiensis, with similarities of up to 99%. The presence of B. cereus could indicate a special relationship between this microorganism and WBD. However, it is imperative to carry out more studies on identifying the bacteria associated with this disease to support these findings.
KW - Coastal ecosystems
KW - coral disease
KW - coral nursery
UR - http://www.scopus.com/inward/record.url?scp=85139995263&partnerID=8YFLogxK
U2 - 10.18687/LACCEI2022.1.1.714
DO - 10.18687/LACCEI2022.1.1.714
M3 - Conference contribution
AN - SCOPUS:85139995263
T3 - Proceedings of the LACCEI international Multi-conference for Engineering, Education and Technology
BT - 20th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology
A2 - Larrondo Petrie, Maria M.
A2 - Texier, Jose
A2 - Pena, Andrea
A2 - Viloria, Jose Angel Sanchez
PB - Latin American and Caribbean Consortium of Engineering Institutions
T2 - 20th LACCEI International Multi-Conference for Engineering, Education Caribbean Conference for Engineering and Technology, LACCEI 2022
Y2 - 18 July 2022 through 22 July 2022
ER -