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Inhibitory effect of sodium dodecyl sulfate in detection of Mycobacterium tuberculosis by amplification of rRNA

  • J. M. Manterola
  • , F. Gamboa
  • , J. Lonca
  • , L. Matas
  • , J. R. Manzano
  • , C. Rodrigo
  • , V. Ausina

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The concentrations of sodium dodecyl (lauryl) sulfate (SDS) found in the sediments of the respiratory samples pretreated with SDS-NaOH varied between 3.36 and 12.42 mg/ml. These concentrations of SDS were higher than the level considered critical (> or = 0.16 mg/ml) to obtain negative results with the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Extensive washing, after neutralization of sediment, is sufficient to remove all traces of detergent which interfere with the enzymes used by the Mycobacterium Tuberculosis Direct Test assay.
Translated title of the contributionEfecto inhibidor del dodecil sulfato de sodio en la detección de Mycobacterium tuberculosis mediante amplificación de ARNr
Original languageEnglish
Pages (from-to)3338-3340
Number of pages3
JournalJournal of Clinical Microbiology
Volume33
Issue number12
DOIs
StatePublished - 01 Dec 1995
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Bacteria (microorganisms)
  • Mycobacterium
  • Mycobacterium tuberculosis

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