Influence of Hydroxyapatite and Gelatin Content on Crosslinking Dynamics and HDFn Cell Viability in Alginate Bioinks for 3D Bioprinting

Lina Maria Anaya-Sampayo, Nelly S. Roa, Constanza Martínez-Cardozo, Dabeiba Adriana García-Robayo, Luis M. Rodríguez-Lorenzo

Research output: Contribution to journalArticlepeer-review

Abstract

This study investigates how varying concentrations of hydroxyapatite (OHAp) and the addition of gelatin influence the ionic crosslinking time of alginate-based bioinks, as well as the shear stress experienced by neonatal human dermal fibroblasts (HDFn) during extrusion. These factors are crucial for validating bioinks and developing viable 3D bioprinted models. Four bioink formulations were created with a 50/50 ratio of alginate to gelatin, incorporating different calcium phosphate concentrations (0%, 1%, 5%, and 10%). The bioink compositions were confirmed via Fourier Transform Infrared (FT-IR) spectroscopy, and rheological analyses evaluated their pseudoplastic behavior, printability limits, and crosslinking times. The results indicated a notable increase in the consistency index (k) from 0.32 for the 0% OHAp formulation to 0.48 for the 10% OHAp formulation, suggesting improved viscoelastic properties. The elastic modulus recovery after crosslinking rose significantly from 245 Pa to 455 Pa. HDFn experienced a shear stress of up to 1.5436 Pa at the tip during extrusion with the HDFn-ALG5-GEL5-OHAp10 bioinks, calculated at a shear rate as low as 2 s−1. Viability assays confirmed over 70% cell viability 24 h post-extrusion and 92% viability after 7 days for the 10% OHAp formulation, highlighting the potential of hydroxyapatite-enhanced bioinks in tissue engineering applications.

Translated title of the contributionInfluencia del contenido de hidroxiapatita y gelatina en la dinámica de reticulación y la viabilidad celular de HDFn en biotintas de alginato para bioimpresión 3D
Original languageEnglish
Article number3224
Number of pages18
JournalPolymers
Volume16
Issue number22
DOIs
StatePublished - 20 Nov 2024

Keywords

  • bioinks
  • 3D bioprinting
  • alginate
  • gelatin
  • hydroxyapatite
  • cell viability
  • shear stress

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