TY - JOUR
T1 - Increased Resolvin E1 Production by Peripheral Blood Mononuclear Cells in Periodontitis Patients
T2 - Pilot Study
AU - Suárez, Lina J.
AU - González-Duarte, Wilmer
AU - Torrez-Velasco, Rodrigo
AU - Salinas, Viviana
AU - Roa-Molina, Nelly S.
AU - Muñoz, Sindy M.
AU - Rodríguez, Luz-Stella
AU - Arce, Roger M.
AU - Shibli, Jamil A.
AU - Rodríguez-Ciodaro, Adriana
N1 - Publisher Copyright:
© 2024, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.
PY - 2024/10/22
Y1 - 2024/10/22
N2 - This study quantified the production of the pro-resolving agent Resolvin E1 by peripheral blood mononuclear cells (PBMC) from 20 systemically healthy volunteers with and without periodontitis after stimulation with lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg). Ten periodontitis patients and 10 healthy volunteers (30-50 years old), matched by age and sex, were recruited. Peripheral blood mononuclear cells were isolated and stimulated in culture plates for 24 hours with Pg LPS. Resolvin E1 levels were measured in the supernatants by enzyme-linked immunosorbent assay. Significantly higher production of Resolvin E1 was observed in both groups when stimulated with LPS compared to baseline levels (p<0.001). A significant increase in Resolvin E1 was observed in the presence of Lipopolysaccharide in the patients with periodontitis compared to the healthy group (p=0.0019). Resolvin E1 levels may reflect a measure of resolution of inflammation that warrants further clinical investigation.
AB - This study quantified the production of the pro-resolving agent Resolvin E1 by peripheral blood mononuclear cells (PBMC) from 20 systemically healthy volunteers with and without periodontitis after stimulation with lipopolysaccharide (LPS) from Porphyromonas gingivalis (Pg). Ten periodontitis patients and 10 healthy volunteers (30-50 years old), matched by age and sex, were recruited. Peripheral blood mononuclear cells were isolated and stimulated in culture plates for 24 hours with Pg LPS. Resolvin E1 levels were measured in the supernatants by enzyme-linked immunosorbent assay. Significantly higher production of Resolvin E1 was observed in both groups when stimulated with LPS compared to baseline levels (p<0.001). A significant increase in Resolvin E1 was observed in the presence of Lipopolysaccharide in the patients with periodontitis compared to the healthy group (p=0.0019). Resolvin E1 levels may reflect a measure of resolution of inflammation that warrants further clinical investigation.
KW - Leukocytes Mononuclear cells
KW - Periodontitis
KW - Pro-resolving lipid mediators
KW - Trained innate immunity
KW - Porphyromonas gingivalis
KW - Periodontitis/microbiology
KW - Eicosapentaenoic Acid/analogs & derivatives
KW - Enzyme-Linked Immunosorbent Assay
KW - Humans
KW - Middle Aged
KW - Male
KW - Case-Control Studies
KW - Lipopolysaccharides
KW - Pilot Projects
KW - Adult
KW - Female
KW - Leukocytes, Mononuclear/metabolism
UR - https://doi.org/10.1590/0103-6440202405861
UR - https://www.mendeley.com/catalogue/2fea20c4-90b7-3e21-806e-ba3d6e8d7773/
UR - http://www.scopus.com/inward/record.url?scp=85208164155&partnerID=8YFLogxK
U2 - 10.1590/0103-6440202405861
DO - 10.1590/0103-6440202405861
M3 - Article
C2 - 39476108
SN - 0103-6440
VL - 35
SP - 1
EP - 8
JO - Brazilian Dental Journal
JF - Brazilian Dental Journal
M1 - e24-5861
ER -