TY - JOUR
T1 - In vitro mineral apposition analysis of two Colombian plant extracts on Amelogenesis imperfecta teeth
AU - Gutiérrez-Prieto, Sandra J.
AU - Sequeda-Castañeda, Luis G.
AU - Penedo-Jaramillo, Gabriela M.
AU - Chacín-Nieto, Andrea V.
AU - Contreras-Cáceres, Daniel R.
AU - Moreno-Abello, Gloria C.
AU - Galvis-Rincón, María P.
AU - Gamboa-Jaimes, Fredy O.
AU - Luengas-Caicedo, Pilar E.
N1 - Publisher Copyright:
© 2021 The Authors. Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.
PY - 2022/2
Y1 - 2022/2
N2 - Objective: To determine if native Colombian Piper marginatum Jacq. and Ilex guayusa Loes plant extracts have a remineralizing effect on teeth with Amelogenesis imperfecta in comparison with the commercial products Clinpro-3M and Recaldent™. Material and Methods: An in vitro study was carried out with 128 human teeth slices (64 healthy and 64 with Amelogenesis imperfecta) on which an initial Raman spectroscopy was performed followed by Raman spectroscopies at 0, 24, 48, and 72 h to determine possible remineralization by observing mineral increase or decrease as a result of P. marginatum Jacq. and I. guayusa Loes extract application in comparison to control substance (Clinpro and Recaldent™) application. Obtained data were analyzed using a bivariate method with a t unidirectional test. Significant differences among groups were determined by an ANOVA with Dunnett post hoc tests. Results: Native I. guayusa Loes and P. marginatum Jacq. Colombian plants extracts exhibited phosphate and orthophosphate mineral apposition, where P. marginatum Jacq. presented better results. Conclusions: Native Colombian I. guayusa Loes and P. marginatum Jacq plant extract might in the future be useful for dental tissue remineralization, as they induced phosphate and orthophosphate mineral apposition, main components of tooth enamel. These types of natural compounds can become an alternative to fluorine, whose ingestion is harmful to the human body.
AB - Objective: To determine if native Colombian Piper marginatum Jacq. and Ilex guayusa Loes plant extracts have a remineralizing effect on teeth with Amelogenesis imperfecta in comparison with the commercial products Clinpro-3M and Recaldent™. Material and Methods: An in vitro study was carried out with 128 human teeth slices (64 healthy and 64 with Amelogenesis imperfecta) on which an initial Raman spectroscopy was performed followed by Raman spectroscopies at 0, 24, 48, and 72 h to determine possible remineralization by observing mineral increase or decrease as a result of P. marginatum Jacq. and I. guayusa Loes extract application in comparison to control substance (Clinpro and Recaldent™) application. Obtained data were analyzed using a bivariate method with a t unidirectional test. Significant differences among groups were determined by an ANOVA with Dunnett post hoc tests. Results: Native I. guayusa Loes and P. marginatum Jacq. Colombian plants extracts exhibited phosphate and orthophosphate mineral apposition, where P. marginatum Jacq. presented better results. Conclusions: Native Colombian I. guayusa Loes and P. marginatum Jacq plant extract might in the future be useful for dental tissue remineralization, as they induced phosphate and orthophosphate mineral apposition, main components of tooth enamel. These types of natural compounds can become an alternative to fluorine, whose ingestion is harmful to the human body.
KW - Amelogenesis imperfecta
KW - Colombia
KW - medicinal plant
KW - mineral apposition
UR - http://www.scopus.com/inward/record.url?scp=85114486932&partnerID=8YFLogxK
U2 - 10.1002/cre2.485
DO - 10.1002/cre2.485
M3 - Article
C2 - 34498426
AN - SCOPUS:85114486932
SN - 2057-4347
VL - 8
SP - 336
EP - 349
JO - Clinical and Experimental Dental Research
JF - Clinical and Experimental Dental Research
IS - 1
ER -