hTERT Protein Expression in Cytoplasm and Nucleus and its Association with HPV Infection in Patients with Cervical Cancer

Pablo Moreno-Acosta; Mónica Molano; Nicolas Morales; Jinneth Acosta; Cristian González-Prieto; Diana Mayorga; Lina Buitrago; Oscar Gamboa; Juan Carlos Mejía; July Castro; Alfredo Romero-Rojas; Sophie Espenel; Gerald L. Murray; Suzanne M. Garland; Alexis Vallard; Nicolas Magné

doi:10.21873/CGP.20218

hTERT Protein Expression in Cytoplasm and Nucleus and its Association with HPV Infection in Patients with Cervical Cancer

Pablo Moreno-Acosta
, Mónica Molano
, Nicolas Morales
, Jinneth Acosta
, Cristian González-Prieto
, Diana Mayorga
, Lina Buitrago
, Oscar Gamboa
, Juan Carlos Mejía
, July Castro
, Alfredo Romero-Rojas
, Sophie Espenel
, Gerald L. Murray
, Suzanne M. Garland
, Alexis Vallard
, Nicolas Magné

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Background: Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. Patients and Methods: One hundred seventy-three patients with cervical cancer were analyzed. hTERT protein expression was detected by immunohistochemistry. hTERT DNA methylation analysis was performed using a PCR-RLB-hTERT assay, targeting two regions of the hTERT promoter. Type specific HPV infection was detected by using GP5+/GP6+PCR-RLB. Results: hTERT protein expression was found in both cytoplasm and nucleus (78.0% of the samples showed a cytoplasmic localization and 79.8%

Original language	English
Pages (from-to)	615-625
Number of pages	11
Journal	Cancer Genomics and Proteomics
Volume	17
Issue number	5
DOIs	https://doi.org/10.21873/CGP.20218
State	Published - 2020
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cervical cancer
Cytoplasmic hTERT
HPV
HTERT
HTERT methylation
HTERT protein expression
Localization
Nuclear hTERT

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10.21873/CGP.20218

Cite this

Moreno-Acosta, P., Molano, M., Morales, N., Acosta, J., González-Prieto, C., Mayorga, D., Buitrago, L., Gamboa, O., Carlos Mejía, J., Castro, J., Romero-Rojas, A., Espenel, S., Murray, G. L., Garland, S. M., Vallard, A., & Magné, N. (2020). hTERT Protein Expression in Cytoplasm and Nucleus and its Association with HPV Infection in Patients with Cervical Cancer. Cancer Genomics and Proteomics, 17(5), 615-625. https://doi.org/10.21873/CGP.20218

@article{8b89c94a20cb4c279370c8ef7405802c,

title = "hTERT Protein Expression in Cytoplasm and Nucleus and its Association with HPV Infection in Patients with Cervical Cancer",

abstract = "Background: Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. Patients and Methods: One hundred seventy-three patients with cervical cancer were analyzed. hTERT protein expression was detected by immunohistochemistry. hTERT DNA methylation analysis was performed using a PCR-RLB-hTERT assay, targeting two regions of the hTERT promoter. Type specific HPV infection was detected by using GP5+/GP6+PCR-RLB. Results: hTERT protein expression was found in both cytoplasm and nucleus (78.0\% of the samples showed a cytoplasmic localization and 79.8\%",

keywords = "Cervical cancer, Cytoplasmic hTERT, HPV, HTERT, HTERT methylation, HTERT protein expression, Localization, Nuclear hTERT",

author = "Pablo Moreno-Acosta and M{\'o}nica Molano and Nicolas Morales and Jinneth Acosta and Cristian Gonz{\'a}lez-Prieto and Diana Mayorga and Lina Buitrago and Oscar Gamboa and \{Carlos Mej{\'i}a\}, Juan and July Castro and Alfredo Romero-Rojas and Sophie Espenel and Murray, \{Gerald L.\} and Garland, \{Suzanne M.\} and Alexis Vallard and Nicolas Magn{\'e}",

year = "2020",

doi = "10.21873/CGP.20218",

language = "English",

volume = "17",

pages = "615--625",

journal = "Cancer Genomics and Proteomics",

issn = "1109-6535",

publisher = "International Institute of Anticancer Research",

number = "5",

}

Moreno-Acosta, P, Molano, M, Morales, N, Acosta, J, González-Prieto, C, Mayorga, D, Buitrago, L, Gamboa, O, Carlos Mejía, J, Castro, J, Romero-Rojas, A, Espenel, S, Murray, GL, Garland, SM, Vallard, A & Magné, N 2020, 'hTERT Protein Expression in Cytoplasm and Nucleus and its Association with HPV Infection in Patients with Cervical Cancer', Cancer Genomics and Proteomics, vol. 17, no. 5, pp. 615-625. https://doi.org/10.21873/CGP.20218

TY - JOUR

T1 - hTERT Protein Expression in Cytoplasm and Nucleus and its Association with HPV Infection in Patients with Cervical Cancer

AU - Moreno-Acosta, Pablo

AU - Molano, Mónica

AU - Morales, Nicolas

AU - Acosta, Jinneth

AU - González-Prieto, Cristian

AU - Mayorga, Diana

AU - Buitrago, Lina

AU - Gamboa, Oscar

AU - Carlos Mejía, Juan

AU - Castro, July

AU - Romero-Rojas, Alfredo

AU - Espenel, Sophie

AU - Murray, Gerald L.

AU - Garland, Suzanne M.

AU - Vallard, Alexis

AU - Magné, Nicolas

PY - 2020

Y1 - 2020

N2 - Background: Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. Patients and Methods: One hundred seventy-three patients with cervical cancer were analyzed. hTERT protein expression was detected by immunohistochemistry. hTERT DNA methylation analysis was performed using a PCR-RLB-hTERT assay, targeting two regions of the hTERT promoter. Type specific HPV infection was detected by using GP5+/GP6+PCR-RLB. Results: hTERT protein expression was found in both cytoplasm and nucleus (78.0% of the samples showed a cytoplasmic localization and 79.8%

AB - Background: Few studies have analyzed the association between human telomerase reverse transcriptase (hTERT) protein expression (nuclear and cytoplasmic localization), hTERT methylation status, and human papillomavirus (HPV) genotype infection in cervical cancer. Patients and Methods: One hundred seventy-three patients with cervical cancer were analyzed. hTERT protein expression was detected by immunohistochemistry. hTERT DNA methylation analysis was performed using a PCR-RLB-hTERT assay, targeting two regions of the hTERT promoter. Type specific HPV infection was detected by using GP5+/GP6+PCR-RLB. Results: hTERT protein expression was found in both cytoplasm and nucleus (78.0% of the samples showed a cytoplasmic localization and 79.8%

KW - Cervical cancer

KW - Cytoplasmic hTERT

KW - HPV

KW - HTERT

KW - HTERT methylation

KW - HTERT protein expression

KW - Localization

KW - Nuclear hTERT

UR - https://www.scopus.com/pages/publications/85090175001

U2 - 10.21873/CGP.20218

DO - 10.21873/CGP.20218

M3 - Article

C2 - 32859640

AN - SCOPUS:85090175001

SN - 1109-6535

VL - 17

SP - 615

EP - 625

JO - Cancer Genomics and Proteomics

JF - Cancer Genomics and Proteomics

IS - 5

ER -

hTERT Protein Expression in Cytoplasm and Nucleus and its Association with HPV Infection in Patients with Cervical Cancer

Abstract

UN SDGs

Keywords

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