Genotyping of Malassezia pachydermatis disclosed genetic variation in isolates from dogs in Colombia1

Adriana Marcela Celis-Ramírez, Marcela Guevara-Suarez, Juan Camilo Galvis-Marín, María Ximena Rodríguez-Bocanegra, Rubiela Castañeda-Salazar, Melva Yomary Linares-Linares, Sergio Heli Triana-Sierra, Adriana del Pilar Pulido-Villamarín

Research output: Contribution to journalArticlepeer-review

Abstract

Malassezia pachydermatis is a lipophilic and lipid-dependent yeast mostly isolated from animals’ skin; hence, it is regarded as a zoophilic species causing otitis externa in dogs. Aspects associated with its epidemiology and pathogenicity is a matter of interest. This study aimed to conduct a molecular characterization of 43 isolates of M. pachydermatis obtained from dogs with otitis externa. For this purpose, the 5.8S internal transcribed spacer 2 (ITS2) and D1/D2 26S rRNA regions were amplified, sequenced and analyzed using restriction fragment length polymorphism (RFLP) with AluI, CfoI, and BstF5I endonucleases. Phylogenetic analyses revealed that these isolates grouped with the sequence types I, IV and V, previously proposed for M. pachydermatis. Interestingly, we found a new polymorphic RFLP pattern using BstF5I, these isolates were associated with the sequence types IV and V, nevertheless an association between polymorphic RFLP patterns, and fosfolipase activity or canine population data was not observed. These findings underline the genetic diversity of M. pachydermatis and provide new insights about the epidemiology of this species in the analyzed population.

Original languageEnglish
Pages (from-to)915-922
Number of pages8
JournalPesquisa Veterinaria Brasileira
Volume39
Issue number11
DOIs
StatePublished - 2019

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