Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR

Dabeiba A. García; Markus Schmitt; Ángel Cid-Arregui; Marcos Castillo; Ignacio Briceño; Fabio A. Aristizábal

doi:10.18597/rcog.253

Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR

Translated title of the contribution: Genotyping the human papilloma virus (HPV) in cytobrush samples taken from patients attending different hospitals in bogotá (Colombia) and evaluating agreement between two PCR-based methods

Dabeiba A. García
, Markus Schmitt
, Ángel Cid-Arregui
, Marcos Castillo
, Ignacio Briceño
, Fabio A. Aristizábal

Department of Oral System

German Cancer Research Center
Universidad de la Sabana
Dinamica IPS
Universidad Nacional de Colombia

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective: genotyping HPV in samples taken from patients having different histopathological degrees of cervical injury. Materials and methods: this was a cross-sectional study of cervical scrape samples taken from females having different degrees of histopathological cervical injury in Bogotá during 2009. Luminex® xMAP® was used, which is a highly sensitive, reproducible and automated technique, as well as reverse line blot (RLB). Results: infections caused by HPV types 16 and 18 were the most frequently found types of pathology of the cervix. Mixed infections predominated (78.04%); amongst cases having negative pathology, 85.7% was found for cervical intraepithelial neoplasia (CIN) I, 81.25% for CIN II, 78.78% for CIN III and 71.42% for cervical cancer. Agreement between both methods was 67%. Conclusion: infection by VPH types 16 and 18 predominated in the population being studied.

Translated title of the contribution	Genotyping the human papilloma virus (HPV) in cytobrush samples taken from patients attending different hospitals in bogotá (Colombia) and evaluating agreement between two PCR-based methods
Original language	Spanish
Pages (from-to)	310-318
Number of pages	9
Journal	Revista Colombiana de Obstetricia y Ginecologia
Volume	61
Issue number	4
DOIs	https://doi.org/10.18597/rcog.253
State	Published - 2010

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SDG 3 Good Health and Well-being

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10.18597/rcog.253

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García, D. A., Schmitt, M., Cid-Arregui, Á., Castillo, M., Briceño, I., & Aristizábal, F. A. (2010). Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR. Revista Colombiana de Obstetricia y Ginecologia, 61(4), 310-318. https://doi.org/10.18597/rcog.253

@article{6beccae833384a949e5a270d1b9ae682,

title = "Genotipificaci{\'o}n del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogot{\'a} y evalua ci{\'o}n de la concordan cia de dos m{\'e}todos basados en PCR",

abstract = "Objective: genotyping HPV in samples taken from patients having different histopathological degrees of cervical injury. Materials and methods: this was a cross-sectional study of cervical scrape samples taken from females having different degrees of histopathological cervical injury in Bogot{\'a} during 2009. Luminex{\textregistered} xMAP{\textregistered} was used, which is a highly sensitive, reproducible and automated technique, as well as reverse line blot (RLB). Results: infections caused by HPV types 16 and 18 were the most frequently found types of pathology of the cervix. Mixed infections predominated (78.04\%); amongst cases having negative pathology, 85.7\% was found for cervical intraepithelial neoplasia (CIN) I, 81.25\% for CIN II, 78.78\% for CIN III and 71.42\% for cervical cancer. Agreement between both methods was 67\%. Conclusion: infection by VPH types 16 and 18 predominated in the population being studied.",

keywords = "Cervical dysplasia, Molecular biology, Papilloma virus, Pathology",

author = "Garc{\'i}a, \{Dabeiba A.\} and Markus Schmitt and {\'A}ngel Cid-Arregui and Marcos Castillo and Ignacio Brice{\~n}o and Aristiz{\'a}bal, \{Fabio A.\}",

year = "2010",

doi = "10.18597/rcog.253",

language = "Espa{\~n}ol ",

volume = "61",

pages = "310--318",

journal = "Revista Colombiana de Obstetricia y Ginecologia",

issn = "0034-7434",

publisher = "Federacion Colombiana de Asociaciones de Obstetricia y Ginecologia (FECOLSOG)",

number = "4",

}

García, DA, Schmitt, M, Cid-Arregui, Á, Castillo, M, Briceño, I & Aristizábal, FA 2010, 'Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR', Revista Colombiana de Obstetricia y Ginecologia, vol. 61, no. 4, pp. 310-318. https://doi.org/10.18597/rcog.253

Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR. / García, Dabeiba A.; Schmitt, Markus; Cid-Arregui, Ángel et al.
In: Revista Colombiana de Obstetricia y Ginecologia, Vol. 61, No. 4, 2010, p. 310-318.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR

AU - García, Dabeiba A.

AU - Schmitt, Markus

AU - Cid-Arregui, Ángel

AU - Castillo, Marcos

AU - Briceño, Ignacio

AU - Aristizábal, Fabio A.

PY - 2010

Y1 - 2010

N2 - Objective: genotyping HPV in samples taken from patients having different histopathological degrees of cervical injury. Materials and methods: this was a cross-sectional study of cervical scrape samples taken from females having different degrees of histopathological cervical injury in Bogotá during 2009. Luminex® xMAP® was used, which is a highly sensitive, reproducible and automated technique, as well as reverse line blot (RLB). Results: infections caused by HPV types 16 and 18 were the most frequently found types of pathology of the cervix. Mixed infections predominated (78.04%); amongst cases having negative pathology, 85.7% was found for cervical intraepithelial neoplasia (CIN) I, 81.25% for CIN II, 78.78% for CIN III and 71.42% for cervical cancer. Agreement between both methods was 67%. Conclusion: infection by VPH types 16 and 18 predominated in the population being studied.

AB - Objective: genotyping HPV in samples taken from patients having different histopathological degrees of cervical injury. Materials and methods: this was a cross-sectional study of cervical scrape samples taken from females having different degrees of histopathological cervical injury in Bogotá during 2009. Luminex® xMAP® was used, which is a highly sensitive, reproducible and automated technique, as well as reverse line blot (RLB). Results: infections caused by HPV types 16 and 18 were the most frequently found types of pathology of the cervix. Mixed infections predominated (78.04%); amongst cases having negative pathology, 85.7% was found for cervical intraepithelial neoplasia (CIN) I, 81.25% for CIN II, 78.78% for CIN III and 71.42% for cervical cancer. Agreement between both methods was 67%. Conclusion: infection by VPH types 16 and 18 predominated in the population being studied.

KW - Cervical dysplasia

KW - Molecular biology

KW - Papilloma virus

KW - Pathology

UR - https://www.scopus.com/pages/publications/79953216055

U2 - 10.18597/rcog.253

DO - 10.18597/rcog.253

M3 - Artículo

AN - SCOPUS:79953216055

SN - 0034-7434

VL - 61

SP - 310

EP - 318

JO - Revista Colombiana de Obstetricia y Ginecologia

JF - Revista Colombiana de Obstetricia y Ginecologia

IS - 4

ER -

García DA, Schmitt M, Cid-Arregui Á, Castillo M, Briceño I, Aristizábal FA. Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR. Revista Colombiana de Obstetricia y Ginecologia. 2010;61(4):310-318. doi: 10.18597/rcog.253

Genotipificación del virus del papiloma Humano (VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de bogotá y evalua ción de la concordan cia de dos métodos basados en PCR

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